Mouse VCAM-1/CD106 PE-conjugated Antibody

Catalog #: FAB6432P Datasheet / COA / SDS

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FAB6432P

Detection of VCAM‑1/CD106 in bEnd.3 Mouse Cell Line by Flow Cytometry.

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All VCAM-1/CD106 Antibodies

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Mouse VCAM-1/CD106 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse (rm) VCAM-1 in Western blots and ELISAs. In sandwich immunoassays, no cross-reactivity or interference was observed with recombinant human VCAM-1, rmICAM-1, rmE-Selectin, rmL-Selectin or rmP-Selectin.

Source

Monoclonal Rat IgG_2A Clone # 112734

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VCAM-1/CD106
Phe25-Glu698 (predicted)
Accession # P29533

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of VCAM‑1/CD106 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VCAM-1/CD106 PE-conjugated Monoclonal Antibody (Catalog # FAB6432P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: VCAM-1/CD106

VCAM-1 (CD106), an 85-95 kDa member of the immunoglobulin superfamily, is a type I transmembrane glycoprotein expressed by activated endothelial cells and multiple cell types, including osteoblasts, B cells plus plasma cells, select fibroblasts, Kupffer cells, sensory and autonomic neurons, visceral smooth muscle, simple squamous type I alveolar epithelium, and CD133⁺CD24⁺renal stem cells. Its expression is typically induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4, LPAM-1 and CD11c. Over amino acids (aa) 25-698 (the ECD), mouse VCAM-1 shares approximately 75% and 86% aa sequence identity with human and rat VCAM-1, respectively. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4 interaction is thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have also shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Within the context, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name

Vascular Cell Adhesion Molecule 1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Alternate Names

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1