Mouse VEGFR2/KDR/Flk-1 APC-conjugated Antibody Summary
Ala20-Glu762
Accession # P35918
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of VEGF R2/KDR/Flk‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R2/KDR/Flk-1 APC-conjugated Monoclonal Antibody (Catalog # FAB4432A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VEGFR2/KDR/Flk-1
VEGF R2, also known as KDR, Flk-1, and CD309, is a 210-220 kDa type I transmembrane glycoprotein member of the 7-Ig/Class V subfamily that belongs to the tyrosine protein kinase family of molecules (1, 2). It is principally expressed on endothelial cells (EC), but also appears on neurons, mast cells, immature dendritic cells and monocytes (3-6). VEGF R2 serves as the major proangiogenic switch that regulates blood vessel development and repair (1, 2). It is known to bind VEGF-A, -C, and -D. On the cell surface, VEGF R2 either homodimerizes or heterodimerizes with VEGF R3 (1). As a homodimer, it appears to interact with Neuropilin-1 (7). It also interacts with Integrin beta 3 subunit, either through its extracellular domain or cytoplasmic tail (8). This interaction may be necessary for EC activation. Notably, VEGF R2 can also be activated in the absence of a VEGF ligand when under torsion stress and in a complex with CD31 and VE-Cadherin (9). Over amino acids 20-762, mouse VEGF R2 shares 93% and 80% amino acid sequence identity with rat and human VEGF R2, respectively.
- Smith, G.A. et al. (2015) Biosci. Rep. Aug 18 [ePub ahead of print].
- Shibuya, M. (2013) J. Biochem. 153:13.
- Le, X-F. et al. (2008) Cell Cycle 7:3747.
- Okabe, K. et al. (2014) Cell 159:584.
- Detoraki, A. et al. (2009) J. Allergy Clin. Immunol. 123:1142.
- Dong, Z. et al. (2009) Curr. Pharm. Des. 15:365.
- Plein, A. et al. (2014) Microcirculation 21:315.
- Somanath, P.R. et al. (2009) Angiogenesis 12:177.
- DuToit, A. (2015) Nat. Rev. Mol. Cell Biol. 16:268.
Product Datasheets
Citation for Mouse VEGFR2/KDR/Flk-1 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Integrins alpha4beta1 and alphaVbeta3 are Reduced in Endothelial Progenitor Cells from Diabetic Dyslipidemic Mice and May Represent New Targets for Therapy in Aortic Valve Disease
Authors: A Filippi, A Constantin, N Alexandru, G Voicu, CA Constantin, D Rebleanu, M Fenyo, D Simionescu, A Simionescu, I Manduteanu, A Georgescu
Cell Transplant, 2020-01-01;29(0):9636897209462.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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