Home / YM1/Chitinase 3-like 3 / Mouse YM1/Chitinase 3-like 3 Antibody

Mouse YM1/Chitinase 3-like 3 Antibody

Catalog #: MAB2446
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2446
MAB2446-SP
Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot.
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Citations (6)
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Mouse YM1/Chitinase 3-like 3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse YM1/Chitinase 3-like in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 281926
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like
Tyr22-Tyr398
Accession # O35744
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse YM1/Chitinase 3-like 3 antibody by Western Blot. View Larger

Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot. Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse YM1/Chitinase 3-like 3 Monoclonal Antibody (Catalog # MAB2446) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for YM1/Chitinase 3-like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: YM1/Chitinase 3-like 3

Eosinophil Chemotactic Factor L (ECF-L), also known as YM1, is a secreted lectin that binds heparin and G1cN oligomers. It is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins but lacks enzymatic activity.

Entrez Gene IDs
12655 (Mouse)
Alternate Names
AI505981; CHI3L3; Chitinase 3 like 3; Chitinase 3-like 3; ECF-L; YM1

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Citations for Mouse YM1/Chitinase 3-like 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization
    Authors: Liu H, Zhang L, Liu Z et al.
    iScience
  2. IkappaB kinase activity drives fetal lung macrophage maturation along a non-M1/M2 paradigm.
    Authors: Stouch A, Zaynagetdinov R, Barham W, Stinnett A, Slaughter J, Yull F, Hoffman H, Blackwell T, Prince L
    J Immunol, 2014-06-30;193(3):1184-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Protective immunity against pulmonary cryptococcosis is associated with STAT1-mediated classical macrophage activation.
    Authors: Hardison S, Herrera G, Young M, Hole C, Wozniak K, Wormley F
    J Immunol, 2012-09-14;189(8):4060-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Central nervous system fibrosis is associated with fibrocyte-like infiltrates.
    Authors: Aldrich A, Kielian T
    Am. J. Pathol., 2011-10-18;179(6):2952-62.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia.
    Authors: Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH
    Am. J. Pathol., 2008-12-18;174(1):153-63.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Databases for technical aspects of immunohistochemistry-2021 update
    Authors: MOROKI T, MATSUO S, HATAKEYAMA H et al.
    Journal of Toxicologic Pathology

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