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Mouse YM1/Chitinase 3-like 3 DuoSet ELISA

Catalog #: DY2446
12 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY2446
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse YM1 / Chitinase 3-like 3 ELISA Standard Curve
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Product Details
Procedure
Citations (12)
FAQs
Supplemental Products
Reviews (2)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse YM1/Chitinase 3-like 3 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
78.1 - 5,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Chitinase 3-like3/ECF-L. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse YM1 / Chitinase 3-like 3 ELISA Standard Curve View Larger

Mouse YM1 / Chitinase 3-like 3 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: YM1/Chitinase 3-like 3

Chitinase 3-like 3, also known as ECF-L and Ym1, is a secreted 377 amino acid lectin that binds heparin and GlcN oligomers. It is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins, but lacks enzymatic activity.

Entrez Gene IDs:
12655 (Mouse)
Alternate Names:
AI505981; CHI3L3; Chitinase 3 like 3; Chitinase 3-like 3; ECF-L; YM1

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Mouse YM1/Chitinase 3-like 3 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. RELMalpha is Induced in Airway Epithelial Cells by Oncostatin M Without Requirement of STAT6 or IL-6 in Mouse Lungs In Vivo
    Authors: L Ho, A Yip, F Lao, F Botelho, CD Richards
    Cells, 2020-05-27;9(6):.
    Species: Mouse, Transgenic Mouse
    Sample Types: BALF
  2. Inflammasome-Independent Role for NLRP3 in Controlling Innate Antihelminth Immunity and Tissue Repair in the Lung
    Authors: AL Chenery, R Alhallaf, Z Agha, J Ajendra, JE Parkinson, MM Cooper, BHK Chan, RM Eichenberg, LA Dent, AAB Robertson, A Kupz, D Brough, A Loukas, TE Sutherland, JE Allen, PR Giacomin
    J. Immunol., 2019-10-04;203(10):2724-2734.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Long noncoding RNA Malat1 regulates differential activation of macrophage and response to lung injury
    Authors: H Cui, S Banerjee, S Guo, N Xie, J Ge, D Jiang, M Zörnig, VJ Thannickal, G Liu
    JCI Insight, 2019-02-21;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Androgen and Androgen Receptor as Enhancers of M2 Macrophage Polarization in Allergic Lung Inflammation
    Authors: M Becerra-Dí, AB Strickland, A Keselman, NM Heller
    J. Immunol., 2018-10-10;0(0):.
    Species: Mouse
    Sample Types: BALF
  5. Dual role of YM1+ M2 macrophages in allergic lung inflammation
    Authors: C Draijer, P Robbe, CE Boorsma, MN Hylkema, BN Melgert
    Sci Rep, 2018-03-23;8(1):5105.
    Species: Mouse
    Sample Types: BALF
  6. IFN Regulatory Factor 2 Inhibits Expression of Glycolytic Genes and Lipopolysaccharide-Induced Proinflammatory Responses in Macrophages
    Authors: H Cui, S Banerjee, S Guo, N Xie, G Liu
    J. Immunol., 2018-03-21;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Extracellular Vesicles from a Helminth Parasite Suppress Macrophage Activation and Constitute an Effective Vaccine for Protective Immunity
    Authors: G Coakley, JL McCaskill, JG Borger, F Simbari, E Robertson, M Millar, Y Harcus, HJ McSorley, RM Maizels, AH Buck
    Cell Rep, 2017-05-23;19(8):1545-1557.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease
    Authors: SJ Van Dyken, HE Liang, RP Naikawadi, PG Woodruff, PJ Wolters, DJ Erle, RM Locksley
    Cell, 2017-04-20;169(3):497-509.e13.
    Species: Mouse
    Sample Types: BALF
  9. IL-33 delivery induces serous cavity macrophage proliferation independent of interleukin-4 receptor alpha
    Eur J Immunol, 2016-10-01;0(0):.
    Species: Mouse
    Sample Types: Lavage Fluid
  10. Modulation of the mevalonate pathway by akt regulates macrophage survival and development of pulmonary fibrosis.
    Authors: Larson-Casey J, Murthy S, Ryan A, Carter A
    J Biol Chem, 2014-11-05;289(52):36204-19.
    Species: Mouse
    Sample Types: BALF
  11. Blockade of IL-33 release and suppression of type 2 innate lymphoid cell responses by helminth secreted products in airway allergy.
    Authors: McSorley H, Blair N, Smith K, McKenzie A, Maizels R
    Mucosal Immunol, 2014-02-05;7(5):1068-78.
    Species: Mouse
    Sample Types: BALF
  12. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012-07-10;7(0):87-104.
    Species: Mouse
    Sample Types: BALF

FAQs

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Reviews for Mouse YM1/Chitinase 3-like 3 DuoSet ELISA

Average Rating: 5 (Based on 2 Reviews)

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Mouse YM1/Chitinase 3-like 3 DuoSet ELISA
By Anonymous on 05/14/2021
Sample Tested: Serum
Mouse YM1/Chitinase 3-like 3 DuoSet ELISA
By Anonymous on 11/22/2019
Sample Tested: Bone marrow-derived dendritic cells

Excellent kit, easy to follow instructions,products works excellently. Clear, quick, reliable results. Five stars all round.

Mouse YM1/Chitinase 3-like 3 DuoSet ELISA DY2446