Myosin Heavy Chain Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Myosin Heavy Chain in C2C12 Mouse Cell Line. Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Myosin Heavy Chain in Human Skeletal Muscle. Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Myosin Heavy Chain in Mouse Skeletal Muscle. Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse Myosin Heavy Chain by Western Blot Knockdown of BAMBI inhibited myogenic differentiation. All the cell samples were harvested after transfection and myogenic induction for 48 and 96 h. (a) The western blot images of BAMBI and GAPDH; (b) the efficiency of siRNA interference on the mRNA and protein expression of BAMBI; (c) the mRNA expression of MyoD at 48 h and that of MyoG and MyHC at 96 h; (d) the western blot images of MyoD at 48 h, MyHC at 96 h, and their corresponding GAPDH; (e) the protein expression of MyoD at 48 h and MyHC at 96 h; (f) immunofluorescence of MyHC in C2C12 myotubes at 96 h post differentiation, images captured at 100× magnification; (g) the populations of myotubes; (h) the differentiation index; and (i) the myotube fusion index. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Myosin Heavy Chain by Immunocytochemistry/Immunofluorescence Generation of DM1-iPSCs and their differentiation.(A) The strategy of our study: patient iPSCs were passed and differentiated at three different passage numbers into CMs or neurons giving 9 samples (left), or had a MyoD1 vector transfected and were differentiated into myocytes, giving 6 samples (right). The CTG repeat lengths were measured in each sample. (B) Six clones from three different DM1 patients expressed pluripotent stem cell markers (Oct3/4, Nanog and Sox2) in conventional PCR. beta -actin was used as a loading control. (C) Karyotypic analysis of undifferentiated iPSCS (Pt-1B). (D, left) Representative live image of CMs on day 20 (Pt-1B). A video clip is available in Supplementary Video 1. (D, right) FACS analysis of the CMs shown in the picture on the left. The X-axis indicates the percentage of cardiac troponin T (cTnT)-positive cells among the total number of CMs. The Y-axis indicates the autofluorescence of the CMs. (E) Representative immunostaining image of neurons on day 42 (Pt-1B). The left panel shows neurons that expressed Tyrosine Hydroxylase (TH) and Microtubule-associated protein 2 (Map2). The right panel shows neurons that expressed TH and Neuron-specific Class III beta -tubulin (TUJ1). (F) Representative immunostaining image of myocytes on day 7 (Pt-1B). The myocytes expressed Myosin Heavy Chain (MHC). Hoechst stains the nuclei. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep42522), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Myosin Heavy Chain by Western Blot LiCl rescued the inhibitory effect of BAMBI siRNA on C2C12 myogenic differentiation. All the cell samples were harvested after transfection and myogenic induction for 48 and 96 h. (a) The mRNA expression of MyoD at 48 h and that of MyoG and MyHC at 96 h; (b) the western blot images of MyoD, MyHC, and GAPDH; (c) the protein expression of MyoD at 48 h and MyHC at 96 h; (d) immunofluorescence images of MyHC in C2C12 myotubes at 96 h post differentiation, images captured at 100× magnification; (e) the populations of myotubes; (f) the differentiation index and (g) the myotube fusion index. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Myosin Heavy Chain by Immunocytochemistry/Immunofluorescence Differentiated myotubes do not differ significantly between HD- and HF-derived skeletal muscle progenitor cells. (a) At day 7 after stimulation, myotubes were stained for the expression of MyHC with an antibody that recognizes the heavy chain of myosin II (MF20) and markers of slow MYH7 and fast MYH1/MYH2 fibers. Nuclei were labelled with DAPI (blue). Representative images are given for both HF- and HD-derived samples. Scale bars represent 50 μm. (b) Fusion coefficient is calculated as a percent of nuclei incorporated in MF20+ myotubes at day 7 after stimulation, and it does not differ between HD- and HF-derived samples. (c) mRNA expression analysis was performed for key markers of muscle development and metabolism for both HF- and HD-derived samples. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30719048), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Myosin Heavy Chain by Western Blot MiR-106a-5p inhibited the myogenic differentiation of C2C12 myoblasts. (A) Overexpression efficiency of miR-106a-5p 3 days (d) and 5 d post differentiation. NC: negative control; (B) The fluorescent microscopy images of C2C12 cells transfected with FAM-labeled miR-106a-5p agomir (×10). Scale bars = 500 μm; (C) Immunostaining for MyHC (red) and DAPI (blue) on 5 d post differentiation (×20). Scale bars = 100 μM; (D–F) The statistical results of differentiation index, fusion index and the populations of myotubes, respectively;1-3 indicates myotubes with 1, 2 or 3 nucleus, >4 indicates myotubes with 4 more nucleus; (G,H) The mRNA expression of MyoD, MyoG, MyHC on 3 d and 5 d post differentiation; (I,J) The mRNA expression of Myomarker and Myomixer 3 d and 5 d post differentiation; (K) The statistical results of MyoD, MyoG, MyHC proteins in Figure 2L; (L) Western blot analyzed for MyoD, MyoG, MyHC proteins 5 d post differentiation; (M) Protein levels of key molecules in PI3K-AKT pathway in C2C12 cells transfected with miR-106a-5p agomir or NC on 5 d post differentiation; (N) The statistical analysis of phosphorylated PI3K (p85 alpha ), AKT (sre473) and mTOR (ser2448). Data were presented as mean ± SEM. n = 3 per group. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30004470), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Myosin Heavy Chain Antibody by Immunocytochemistry/ Immunofluorescence Generation of DM1-iPSCs and their differentiation.(A) The strategy of our study: patient iPSCs were passed and differentiated at three different passage numbers into CMs or neurons giving 9 samples (left), or had a MyoD1 vector transfected and were differentiated into myocytes, giving 6 samples (right). The CTG repeat lengths were measured in each sample. (B) Six clones from three different DM1 patients expressed pluripotent stem cell markers (Oct3/4, Nanog and Sox2) in conventional PCR. beta -actin was used as a loading control. (C) Karyotypic analysis of undifferentiated iPSCS (Pt-1B). (D, left) Representative live image of CMs on day 20 (Pt-1B). A video clip is available in Supplementary Video 1. (D, right) FACS analysis of the CMs shown in the picture on the left. The X-axis indicates the percentage of cardiac troponin T (cTnT)-positive cells among the total number of CMs. The Y-axis indicates the autofluorescence of the CMs. (E) Representative immunostaining image of neurons on day 42 (Pt-1B). The left panel shows neurons that expressed Tyrosine Hydroxylase (TH) and Microtubule-associated protein 2 (Map2). The right panel shows neurons that expressed TH and Neuron-specific Class III beta -tubulin (TUJ1). (F) Representative immunostaining image of myocytes on day 7 (Pt-1B). The myocytes expressed Myosin Heavy Chain (MHC). Hoechst stains the nuclei. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28211918), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myosin Heavy Chain
Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.
Product Datasheets
Citations for Myosin Heavy Chain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
165
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Preclinical testing of the glycogen synthase kinase-3 beta inhibitor tideglusib for rhabdomyosarcoma
Authors: Narendra Bharathy, Matthew N. Svalina, Teagan P. Settelmeyer, Megan M. Cleary, Noah E. Berlow, Susan D. Airhart et al.
Oncotarget
-
Screening method to identify hydrogel formulations that facilitate myotube formation from encapsulated primary myoblasts
Authors: Dhananjay V. Deshmukh, Nils Pasquero, Gajraj Rathore, Joel Zvick, Ori Bar‐Nur, Jurg Dual et al.
Bioengineering & Translational Medicine
-
Smoothelin-Like Protein 1 Regulates the Thyroid Hormone-Induced Homeostasis and Remodeling of C2C12 Cells via the Modulation of Myosin Phosphatase
Authors: Evelin Major, Ilka Keller, Dániel Horváth, István Tamás, Ferenc Erdődi, Beáta Lontay
International Journal of Molecular Sciences
-
The histone deacetylase SIRT6 blocks myostatin expression and development of muscle atrophy
Authors: SA Samant, A Kanwal, VB Pillai, R Bao, MP Gupta
Sci Rep, 2017-09-19;7(1):11877.
-
Filopodia powered by class x myosin promote fusion of mammalian myoblasts
Authors: Hammers DW, Hart CC, Matheny MK et al.
eLife
-
ZAK beta is activated by cellular compression and mediates contraction‐induced MAP kinase signaling in skeletal muscle
Authors: Cathrine Nordgaard, Anna Constance Vind, Amy Stonadge, Rasmus Kjøbsted, Goda Snieckute, Pedro Antas et al.
The EMBO Journal
-
p38 alpha Regulates Expression of DUX4 in a Model of Facioscapulohumeral Muscular Dystrophy
Authors: L. Alejandro Rojas, Erin Valentine, Anthony Accorsi, Joseph Maglio, Ning Shen, Alan Robertson et al.
Journal of Pharmacology and Experimental Therapeutics
-
Laminin mimetic peptide nanofibers regenerate acute muscle defect
Authors: Cagla Eren Cimenci, Gozde Uzunalli, Ozge Uysal, Fatih Yergoz, Ebru Karaca Umay, Mustafa O. Guler et al.
Acta Biomaterialia
-
Ginsenoside Rc, an Active Component of Panax ginseng, Alleviates Oxidative Stress-Induced Muscle Atrophy via Improvement of Mitochondrial Biogenesis
Authors: Aeyung Kim, Sang-Min Park, No Soo Kim, Haeseung Lee
Antioxidants (Basel)
-
HSPB8 frameshift mutant aggregates weaken chaperone-assisted selective autophagy in neuromyopathies
Authors: Barbara Tedesco, Leen Vendredy, Elias Adriaenssens, Marta Cozzi, Bob Asselbergh, Valeria Crippa et al.
Autophagy
-
BAMBI Promotes C2C12 Myogenic Differentiation by Enhancing Wnt/ beta -Catenin Signaling
Authors: Qiangling Zhang, Xin-E Shi, Chengchuang Song, Shiduo Sun, Gongshe Yang, Xiao Li
International Journal of Molecular Sciences
-
Anti-Fibrotic Effect of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells on Skeletal Muscle Cells, Mediated by Secretion of MMP-1
Authors: Alee Choi, Sang Eon Park, Jang Bin Jeong, Suk-joo Choi, Soo-young Oh, Gyu Ha Ryu et al.
International Journal of Molecular Sciences
-
Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line
Authors: Y Kitajima, Y Ono
Skelet Muscle, 2018-08-24;8(1):26.
-
A Systemically Administered Unconjugated Antisense Oligonucleotide Targeting DUX4 Improves Muscular Injury and Motor Function in FSHD Model Mice
Authors: Tetsuhiro Kakimoto, Akira Ogasawara, Kiyoshi Ishikawa, Takashi Kurita, Kumiko Yoshida, Shuichi Harada et al.
Biomedicines
-
Lnc-GD2H Promotes Proliferation by Forming a Feedback Loop With c-Myc and Enhances Differentiation Through Interacting With NACA to Upregulate Myog in C2C12 Myoblasts
Authors: Rui Chen, Si Lei, Yanling She, Shanyao Zhou, Huacai Shi, Cheng Li et al.
Frontiers in Cell and Developmental Biology
-
In vitro exercise model using contractile human and mouse hybrid myotubes
Authors: Weijian Chen, Mazvita R. Nyasha, Masashi Koide, Masahiro Tsuchiya, Naoki Suzuki, Yoshihiro Hagiwara et al.
Scientific Reports
-
A Modified Pre-plating Method for High-Yield and High-Purity Muscle Stem Cell Isolation From Human/Mouse Skeletal Muscle Tissues
Authors: Kiyoshi Yoshioka, Yasuo Kitajima, Narihiro Okazaki, Ko Chiba, Akihiko Yonekura, Yusuke Ono
Frontiers in Cell and Developmental Biology
-
Loss of tafazzin results in decreased myoblast differentiation in C2C12 cells: A myoblast model of Barth syndrome and cardiolipin deficiency
Authors: Wenjia Lou, Christian A. Reynolds, Yiran Li, Jenney Liu, Maik Hüttemann, Michael Schlame et al.
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
-
MicroRNA-106a-5p Inhibited C2C12 Myogenesis via Targeting PIk3R1 and Modulating the PI3k/AkT Signaling.
Authors: Li X, Zhu Y, Zhang H et al.
Genes (Basel).
-
Effectual Endeavors of Silk Protein Sericin against Isoproterenol Induced Cardiac Toxicity and Hypertrophy in Wistar Rats
Authors: Farogh Ahsan, Tarique Mahmood, Tanveer A. Wani, Seema Zargar, Mohammed Haris Siddiqui, Shazia Usmani et al.
Life (Basel)
-
Endothelial protective genes induced by statin are mimicked by ERK5 activation as triggered by a drug combination of FTI-277 and GGTI-298
Authors: Uyen B. Chu, Tyler Duellman, Sara J. Weaver, Yunting Tao, Jay Yang
Biochimica et Biophysica Acta (BBA) - General Subjects
-
Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss.
Authors: Das S, Browne K D et al.
Commun Biol
-
Acute estradiol treatment reduces skeletal muscle protein breakdown markers in early- but not late-postmenopausal women
Authors: Young-Min Park, Amy C. Keller, Shauna S. Runchey, Benjamin F. Miller, Wendy M. Kohrt, Rachael E. Van Pelt et al.
Steroids
-
Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains
Authors: M Di Rienzo, M Antonioli, C Fusco, Y Liu, M Mari, I Orhon, G Refolo, F Germani, M Corazzari, A Romagnoli, F Ciccosanti, B Mandriani, MT Pellico, R De La Torr, H Ding, M Dentice, M Neri, A Ferlini, F Reggiori, M Kulesz-Mar, M Piacentini, G Merla, GM Fimia
Sci Adv, 2019-05-08;5(5):eaau8857.
-
Role of p53 in Cisplatin-Induced Myotube Atrophy
Authors: Matsumoto C, Sekine H, Zhang N et al.
International Journal of Molecular Sciences
-
Preclinical Investigation of Alpinetin in the Treatment of Cancer-Induced Cachexia via Activating PPAR gamma
Authors: Yujie Zhang, Yuxin Zhang, Yichen Li, Li Zhang, Shiying Yu
Frontiers in Pharmacology
-
The ensured proliferative capacity of myoblast in serum-reduced conditions with Methyl-beta -cyclodextrin
Authors: Tomoka Katayama, Yuta Chigi, Daiji Okamura
Frontiers in Cell and Developmental Biology
-
HIF1 alpha -dependent mitophagy facilitates cardiomyoblast differentiation
Authors: Jin-Feng Zhao, Catherine E. Rodger, George F. G. Allen, Simone Weidlich, Ian G. Ganley
Cell Stress
-
Water‐soluble dietary fiber alleviates cancer‐induced muscle wasting through changes in gut microenvironment in mice
Authors: Tomoki Sakakida, Takeshi Ishikawa, Toshifumi Doi, Ryuichi Morita, Yuki Endo, Shinya Matsumura et al.
Cancer Science
-
Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration.
Authors: Park S, Choi Y, Jung N et al.
Int. J. Mol. Med.
-
Chondroitin sulfate E downregulates N-cadherin and suppresses myotube formation
Authors: Fumi SATOH, Akihiro SUGIURA, Jiro TASHIRO, Yoshinao Z. HOSAKA, Katsuhiko WARITA
Journal of Veterinary Medical Science
-
Autophagy induction in the skeletal myogenic differentiation of human tonsil-derived mesenchymal stem cells
Authors: Saeyoung Park, Yoonyoung Choi, Namhee Jung, Jieun Kim, Seiyoon Oh, Yeonsil Yu et al.
International Journal of Molecular Medicine
-
Pericytes in the myovascular niche promote post-natal myofiber growth and satellite cell quiescence
Authors: Enis Kostallari, Yasmine Baba-Amer, Sonia Alonso-Martin, Pamela Ngoh, Frederic Relaix, Peggy Lafuste et al.
Development
-
The secretome of human dental pulp stem cells protects myoblasts from hypoxia induced injury via the Wnt/beta catenin pathway
Authors: Zhang W, Yu L, Han X et al.
Int. J. Mol. Med.
-
Periostin Is Required for the Maintenance of Muscle Fibers during Muscle Regeneration
Authors: Naoki Ito, Yuko Miyagoe-Suzuki, Shin’ichi Takeda, Akira Kudo
International Journal of Molecular Sciences
-
Inflammatory Caspase Activity Mediates HMGB1 Release and Differentiation in Myoblasts Affected by Peripheral Arterial Disease
Authors: Ricardo Ferrari, Bowen Xie, Edwyn Assaf, Kristin Morder, Melanie Scott, Hong Liao et al.
Cells
-
MiR-199-3p enhances muscle regeneration and ameliorates aged muscle and muscular dystrophy
Authors: Masashi Fukuoka, Hiromi Fujita, Kosumo Numao, Yasuko Nakamura, Hideo Shimizu, Masayuki Sekiguchi et al.
Communications Biology
-
Elimination of Mutant mtDNA by an Optimized mpTALEN Restores Differentiation Capacities of Heteroplasmic MELAS-iPSCs
Authors: Yahata N, Boda H, Hata R
Molecular therapy. Methods & clinical development
-
Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions
Authors: Yasuro Furuichi, Yuki Kawabata, Miho Aoki, Yoshitaka Mita, Nobuharu L. Fujii, Yasuko Manabe
Frontiers in Cell and Developmental Biology
-
Glucosamine inhibits myoblast proliferation and differentiation, and stimulates myotube atrophy through distinct signal pathways
Authors: Yu Liu, S;Chen, LK;Chung, YT;Chen, CW;Wu, GL;Chang, YC;Chen, PR;Chang, YI;Lin, HF;Wu, LY;Juan, CC;
The Journal of nutritional biochemistry
Species: Mouse
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Mst1-mediated phosphorylation of FoxO1 and C/EBP-? stimulates cell-protective mechanisms in cardiomyocytes
Authors: Maejima, Y;Nah, J;Aryan, Z;Zhai, P;Sung, EA;Liu, T;Takayama, K;Moghadami, S;Sasano, T;Li, H;Sadoshima, J;
Nature communications
Species: Rat
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Generation of musculoskeletal cells from human urine epithelium-derived presomitic mesoderm cells
Authors: Gao, H;Huang, X;Cai, Z;Cai, B;Wang, K;Li, J;Kuang, J;Wang, B;Zhai, Z;Ming, J;Cao, S;Qin, Y;Pei, D;
Cell & bioscience
Species: Human, Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, Immunohistochemistry -
PHF2 regulates sarcomeric gene transcription in myogenesis
Authors: Fukushima, T;Hasegawa, Y;Kuse, S;Fujioka, T;Nikawa, T;Masubuchi, S;Sakakibara, I;
PloS one
Species: Mouse
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Triptolide, a Cancer Cell Proliferation Inhibitor, Causes Zebrafish Muscle Defects by Regulating Notch and STAT3 Signaling Pathways
Authors: Lee, B;Park, Y;Lee, Y;Kwon, S;Shim, J;
International journal of molecular sciences
Species: Mouse, Fish - Danio rerio (Zebrafish)
Sample Types: Whole Cells, Embryo
Applications: Immunohistochemistry, Immunocytochemistry -
Methionine availability influences essential H3K36me3 dynamics during cell differentiation
Authors: Sun, Y;Ramesh, V;Wei, F;Locasale, JW;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Apabetalone, a Clinical-Stage, Selective BET Inhibitor, Opposes DUX4 Target Gene Expression in Primary Human FSHD Muscle Cells
Authors: Sarsons, CD;Gilham, D;Tsujikawa, LM;Wasiak, S;Fu, L;Rakai, BD;Stotz, SC;Carestia, A;Sweeney, M;Kulikowski, E;
Biomedicines
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Diosgenin prevents dexamethasone-induced myotube atrophy in C2C12?cells
Authors: Yoshioka, Y;Oishi, S;Onoda, K;Shibata, K;Miyoshi, N;
Archives of biochemistry and biophysics
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Western Blot, ICC -
Effects of the purified dry extract of fermented ginseng BST204 on muscle fiber regeneration
Authors: Jo, SI;Park, YS;Chang, Y;Moon, JH;Lee, S;Lee, H;Kim, M;Kim, DY;Bae, S;Park, SY;Yun, H;You, JE;Im, M;Han, HJ;Kim, SY;Jin, DH;
Biochemistry and biophysics reports
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
BRD4 isoforms have distinct roles in tumor progression and metastasis in embryonal rhabdomyosarcoma
Authors: Das, D;Leung, JY;Tergaonkar, V;Loh, AHP;Chiang, CM;Taneja, R;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells, Transfected Whole Cells
Applications: Immunocytochemistry -
MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1
Authors: Cao, H;Du, T;Li, C;Wu, L;Liu, J;Guo, Y;Li, X;Yang, G;Jin, J;Shi, X;
BMC genomics
Species: Mouse
Sample Types: Cell Lysates, Transfected Whole Cells
Applications: Western Blot, Immunocytochemistry -
S100A8, S100A9 and S100A8/A9 heterodimer as novel cachexigenic factors for pancreatic cancer-induced cachexia
Authors: Liao, WC;Chen, CT;Tsai, YS;Wang, XY;Chang, YT;Wu, MS;Chow, LP;
BMC cancer
Species: Mouse
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Ursolic Acid Alleviates Cancer Cachexia and Prevents Muscle Wasting via Activating SIRT1
Authors: Tao, W;Ouyang, Z;Liao, Z;Li, L;Zhang, Y;Gao, J;Ma, L;Yu, S;
Cancers
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Evaluation of Human-Induced Pluripotent Stem Cells Derived from a Patient with Schwartz-Jampel Syndrome Revealed Distinct Hyperexcitability in the Skeletal Muscles
Authors: Y Yamashita, S Nakada, K Nakamura, H Sakurai, K Ohno, T Goto, Y Mabuchi, C Akazawa, N Hattori, E Arikawa-Hi
Biomedicines, 2023-03-07;11(3):.
Species: Mouse
Sample Types: Whole Cells
Applications: IF -
Cardiolipin metabolism regulates expression of muscle transcription factor MyoD1 and muscle development
Authors: L Vo, MW Schmidtke, NT Da Rosa-Ju, M Ren, M Schlame, ML Greenberg
The Journal of Biological Chemistry, 2023-02-04;0(0):102978.
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Decrease in the expression of muscle-specific miRNAs, miR-133a and miR-1, in myoblasts with replicative senescence
Authors: K Shintani-I, R Tsurumi, H Ikegaya
PLoS ONE, 2023-01-17;18(1):e0280527.
Species: Mouse
Sample Types: Cell Lysates, Transfected Whole Cells
Applications: ICC, Western Blot -
Mannan Oligosaccharides Promoted Skeletal Muscle Hypertrophy through the Gut Microbiome and Microbial Metabolites in Mice
Authors: Zhao W, Chen L, Tan W et al.
Foods
-
miR-193b-3p Promotes Proliferation of Goat Skeletal Muscle Satellite Cells through Activating IGF2BP1
Authors: L Li, X Zhang, H Yang, X Xu, Y Chen, D Dai, S Zhan, J Guo, T Zhong, L Wang, J Cao, H Zhang
International Journal of Molecular Sciences, 2022-12-12;23(24):.
Species: Goat
Sample Types: Whole Cell
Applications: IHC -
Creatine modulates cellular energy metabolism and protects against cancer cachexia-associated muscle wasting
Authors: L Wei, R Wang, K Lin, X Jin, L Li, J Wazir, W Pu, P Lian, R Lu, S Song, Q Zhao, J Li, H Wang
Frontiers in Pharmacology, 2022-12-07;13(0):1086662.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
MicroRNA-381 Regulates Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells by Targeting PTEN and JAG2
Authors: J Shen, J Wang, H Zhen, Y Liu, L Li, Y Luo, J Hu, X Liu, S Li, Z Hao, M Li, Z Zhao
International Journal of Molecular Sciences, 2022-11-05;23(21):.
Species: Goat
Sample Types: Whole Tissue
Applications: IHC -
Role of lncRNA Has2os in Skeletal Muscle Differentiation and Regeneration
Authors: W Chen, W Chen, P Liu, S Qian, S Tao, M Huang, W Xu, C Li, X Chen, H Lin, Z Qin, J Lu, S Xie
Cells, 2022-11-04;11(21):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Celecoxib impairs primary human myoblast proliferation and differentiation independent of cyclooxygenase 2 inhibition
Authors: RW Matheny, AL Kolb, AV Geddis, BM Roberts
Physiological Reports, 2022-11-01;10(21):e15481.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Protective Effects of the Chalcone-Based Derivative AN07 on Inflammation-Associated Myotube Atrophy Induced by Lipopolysaccharide
Authors: WY Fang, CL Lin, WH Chang, CH Chang, YC Huang, YH Tsai, FR Chang, YC Lo
International Journal of Molecular Sciences, 2022-10-26;23(21):.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
2-Deoxy-D-glucose Alleviates Cancer Cachexia-Induced Muscle Wasting by Enhancing Ketone Metabolism and Inhibiting the Cori Cycle
Authors: L Wei, R Wang, J Wazir, K Lin, S Song, L Li, W Pu, C Zhao, Y Wang, Z Su, H Wang
Cells, 2022-09-25;11(19):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development
Authors: M Ling, X Lai, L Quan, F Li, L Lang, Y Fu, S Feng, X Yi, C Zhu, P Gao, X Zhu, L Wang, G Shu, Q Jiang, S Wang
International Journal of Molecular Sciences, 2022-07-07;23(14):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
COPS3 AS lncRNA enhances myogenic differentiation and maintains fast-type myotube phenotype
Authors: ZZ He, T Zhao, N Qimuge, T Tian, W Yan, X Yi, J Jin, R Cai, T Yu, G Yang, W Pang
Cellular Signalling, 2022-04-28;95(0):110341.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC/IF -
Knockdown of CDR1as Decreases Differentiation of Goat Skeletal Muscle Satellite Cells via Upregulating miR-27a-3p to Inhibit ANGPT1
Authors: B Kyei, E Odame, L Li, L Yang, S Zhan, J Li, Y Chen, D Dai, J Cao, J Guo, T Zhong, L Wang, H Zhang
Genes, 2022-04-09;13(4):.
Species: Goat
Sample Types: Transfected Whole Cells
Applications: ICC -
Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells
Authors: I Kim, A Ghosh, N Bundschuh, L Hinte, E Petrosyan, F von Meyenn, O Bar-Nur
Science Advances, 2022-04-06;8(14):eabj4928.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC/IF -
Tceal5 and Tceal7 Function in C2C12 Myogenic Differentiation via Exosomes in Fetal Bovine Serum
Authors: A Sawada, T Yamamoto, T Sato
International Journal of Molecular Sciences, 2022-02-12;23(4):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration
Authors: X Liu, E Zu, X Chang, X Ma, Z Wang, X Song, X Li, Q Yu, KI Kamei, T Hayashi, K Mizuno, S Hattori, H Fujisaki, T Ikejima, DO Wang
Disease Models & Mechanisms, 2021-12-24;14(12):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
beta-Hydroxy-beta-Methylbutyrate Supplementation Promotes Antitumor Immunity in an Obesity Responsive Mouse Model of Pancreatic Ductal Adenocarcinoma
Authors: MF Coleman, KA Liu, AJ Pfeil, SK Etigunta, X Tang, S Fabela, LM Lashinger, Z Cui, SD Hursting
Cancers, 2021-12-18;13(24):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
VEGFB Promotes Myoblasts Proliferation and Differentiation through VEGFR1-PI3K/Akt Signaling Pathway
Authors: M Ling, L Quan, X Lai, L Lang, F Li, X Yang, Y Fu, S Feng, X Yi, C Zhu, P Gao, X Zhu, L Wang, G Shu, Q Jiang, S Wang
International Journal of Molecular Sciences, 2021-12-12;22(24):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Downregulation of miR-29c promotes muscle wasting by modulating the activity of leukemia inhibitory factor in lung cancer cachexia
Authors: K Xie, H Xiong, W Xiao, Z Xiong, W Hu, J Ye, N Xu, J Shi, C Yuan, Z Chen, D Miao, X Zhang, H Yang
Cancer Cell International, 2021-11-27;21(1):627.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Gene regulation by morpholines and piperidines in the cardiac embryonic stem cell test
Authors: RH Mennen, N Hallmark, M Pallardy, R Bars, H Tinwell, AH Piersma
Toxicology and Applied Pharmacology, 2021-10-29;433(0):115781.
Species: Mouse
Sample Types: Embryonic Bodies
Applications: ICC -
M2 Macrophage-derived exosomal miR-501 contributes to pubococcygeal muscle regeneration
Authors: M Zhou, B Li, C Liu, M Hu, J Tang, J Min, J Cheng, L Hong
International immunopharmacology, 2021-10-08;101(0):108223.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Filopodia powered by class x myosin promote fusion of mammalian myoblasts
Authors: Hammers DW, Hart CC, Matheny MK et al.
eLife
-
Simple derivation of skeletal muscle from human pluripotent stem cells using temperature-sensitive Sendai virus vector
Authors: GW Tan, T Kondo, K Imamura, M Suga, T Enami, A Nagahashi, K Tsukita, I Inoue, J Kawaguchi, T Shu, H Inoue
Journal of Cellular and Molecular Medicine, 2021-09-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The ubiquitin ligase Ozz decreases the replacement rate of embryonic myosin in myofibrils
Authors: E Ichimura, K Ojima, S Muroya, T Suzuki, K Kobayashi, T Nishimura
Physiological Reports, 2021-09-01;9(17):e15003.
Species: Mouse
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Wharton's Jelly-Derived Mesenchymal Stem Cells Reduce Fibrosis in a Mouse Model of Duchenne Muscular Dystrophy by Upregulating microRNA 499
Authors: SE Park, JB Jeong, SJ Oh, SJ Kim, H Kim, A Choi, SJ Choi, SY Oh, GH Ryu, J Lee, HB Jeon, JW Chang
Biomedicines, 2021-08-26;9(9):.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Molecular neural crest cell markers enable discrimination of organophosphates in the murine cardiac embryonic stem cell test
Authors: RH Mennen, N Hallmark, M Pallardy, R Bars, H Tinwell, AH Piersma
Toxicology reports, 2021-07-31;8(0):1513-1520.
Species: Mouse
Sample Types: Embryoid Bodies
Applications: ICC -
Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway
Authors: Y She, C Li, T Jiang, S Lei, S Zhou, H Shi, R Chen
Frontiers in Physiology, 2021-07-08;12(0):659272.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
An integrative transcriptome study reveals Ddit4/Redd1 as a key regulator of cancer cachexia in rodent models
Authors: M Niu, L Li, Z Su, L Wei, W Pu, C Zhao, Y Ding, J Wazir, W Cao, S Song, Q Gao, H Wang
Cell Death & Disease, 2021-06-26;12(7):652.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Emerin Represses STAT3 Signaling through Nuclear Membrane-Based Spatial Control
Authors: B Lee, S Lee, Y Lee, Y Park, J Shim
International Journal of Molecular Sciences, 2021-06-22;22(13):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle
Authors: K Yoshioka, H Nagahisa, F Miura, H Araki, Y Kamei, Y Kitajima, D Seko, J Nogami, Y Tsuchiya, N Okazaki, A Yonekura, S Ohba, Y Sumita, K Chiba, K Ito, I Asahina, Y Ogawa, T Ito, Y Ohkawa, Y Ono
Science Advances, 2021-06-09;7(24):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The Effects of Muscle Cell Aging on Myogenesis
Authors: A Moustogian, A Philippou, O Taso, E Zevolis, M Pappa, A Chatzigeor, M Koutsilier
International Journal of Molecular Sciences, 2021-04-02;22(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Neural stemness contributes to cell tumorigenicity
Authors: L Xu, M Zhang, L Shi, X Yang, L Chen, N Cao, A Lei, Y Cao
Cell & bioscience, 2021-01-19;11(1):21.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
FLNC Expression Level Influences the Activity of TEAD-YAP/TAZ Signaling
Authors: A Knyazeva, A Khudiakov, R Vaz, A Muravyev, K Sukhareva, T Sejersen, A Kostareva
Genes (Basel), 2020-11-13;11(11):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Daily Oral Administration of Protease-Treated Royal Jelly Protects Against Denervation-Induced Skeletal Muscle Atrophy
Authors: T Shirakawa, A Miyawaki, T Matsubara, N Okumura, H Okamoto, N Nakai, T Rojasawast, K Morikawa, A Inoue, A Goto, A Washio, T Tsujisawa, T Kawamoto, S Kokabu
Nutrients, 2020-10-11;12(10):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
The calmodulin redox sensor controls myogenesis
Authors: AW Steil, JW Kailing, CJ Armstrong, DG Walgenbach, JC Klein
PLoS ONE, 2020-09-17;15(9):e0239047.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
LMNA Mutations G232E and R482L Cause Dysregulation of Skeletal Muscle Differentiation, Bioenergetics, and Metabolic Gene Expression Profile
Authors: EV Ignatieva, OA Ivanova, MY Komarova, NV Khromova, DE Polev, AA Kostareva, A Sergushich, RI Dmitrieva
Genes (Basel), 2020-09-07;11(9):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix
Authors: J Lee, A Sutani, R Kaneko, J Takeuchi, T Sasano, T Kohda, K Ihara, K Takahashi, M Yamazoe, T Morio, T Furukawa, F Ishino
Nat Commun, 2020-09-03;11(1):4283.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells
Authors: D Baci, M Chirivì, V Pace, F Maiullari, M Milan, A Rampin, P Somma, D Presutti, S Garavelli, A Bruno, S Cannata, C Lanzuolo, C Gargioli, R Rizzi, C Bearzi
Cells, 2020-06-23;9(6):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Western Blot -
Detachment of cell sheets from clinically ubiquitous cell culture vessels by ultrasonic vibration
Authors: C Imashiro, M Hirano, T Morikura, Y Fukuma, K Ohnuma, Y Kurashina, S Miyata, K Takemura
Sci Rep, 2020-06-11;10(1):9468.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors
Authors: Y Nakatake, SBH Ko, AA Sharov, S Wakabayash, M Murakami, M Sakota, N Chikazawa, C Ookura, S Sato, N Ito, M Ishikawa-H, SS Mak, LM Jakt, T Ueno, K Hiratsuka, M Matsushita, SK Goparaju, T Akiyama, KI Ishiguro, M Oda, N Gouda, A Umezawa, H Akutsu, K Nishimura, R Matoba, O Ohara, MSH Ko
Cell Rep, 2020-05-19;31(7):107655.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Urolithin A exerts antiobesity effects through enhancing adipose tissue thermogenesis in mice
Authors: B Xia, XC Shi, BC Xie, MQ Zhu, Y Chen, XY Chu, GH Cai, M Liu, SZ Yang, GA Mitchell, WJ Pang, JW Wu
PLoS Biol., 2020-03-27;18(3):e3000688.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Corylifol A from Psoralea corylifolia L. Enhances Myogenesis and Alleviates Muscle Atrophy
Authors: Y Han, H Lee, H Li, JH Ryu
Int J Mol Sci, 2020-02-25;21(5):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
Authors: H Morisaka, K Yoshimi, Y Okuzaki, P Gee, Y Kunihiro, E Sonpho, H Xu, N Sasakawa, Y Naito, S Nakada, T Yamamoto, S Sano, A Hotta, J Takeda, T Mashimo
Nat Commun, 2019-12-06;10(1):5302.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The SMYD3 methyltransferase promotes myogenesis by activating the myogenin regulatory network
Authors: R Codato, M Perichon, A Divol, E Fung, A Sotiropoul, A Bigot, JB Weitzman, S Medjkane
Sci Rep, 2019-11-21;9(1):17298.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Distinct cell proliferation, myogenic differentiation, and gene expression in skeletal muscle myoblasts of layer and broiler chickens
Authors: Y Nihashi, K Umezawa, S Shinji, Y Hamaguchi, H Kobayashi, T Kono, T Ono, H Kagami, T Takaya
Sci Rep, 2019-11-11;9(1):16527.
Species: Chicken
Sample Types: Whole Cells
Applications: ICC -
FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis
Authors: G Picchiarel, M Demestre, A Zuko, M Been, J Higelin, S Dieterlé, MA Goy, M Mallik, C Sellier, J Scekic-Zah, L Zhang, A Rosenbohm, C Sijlmans, A Aly, S Mersmann, I Sanjuan-Ru, A Hübers, N Messaddeq, M Wagner, N van Bakel, AL Boutillier, A Ludolph, C Lagier-Tou, TM Boeckers, L Dupuis, E Storkebaum
Nat. Neurosci., 2019-10-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Expression patterns of regulatory lncRNAs and miRNAs in muscular atrophy models induced by starvation in vitro and in vivo
Authors: S Lei, Y She, J Zeng, R Chen, S Zhou, H Shi
Mol Med Rep, 2019-09-10;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: ICC, Western Blot -
Skeletal Muscle Atrophy Was Alleviated by Salidroside Through Suppressing Oxidative Stress and Inflammation During Denervation
Authors: Z Huang, Q Fang, W Ma, Q Zhang, J Qiu, X Gu, H Yang, H Sun
Front Pharmacol, 2019-09-10;10(0):997.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: ICC, Western Blot -
The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells
Authors: SY Lee, J Yang, JH Park, HK Shin, WJ Kim, SY Kim, EJ Lee, I Hwang, CS Lee, J Lee, HS Kim
Mol. Ther., 2019-09-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Therapeutic Approaches in Mitochondrial Dysfunction, Inflammation, and Autophagy in Uremic Cachexia: Role of Aerobic Exercise
Authors: Y Zhang, Y Liu, X Bi, C Hu, F Ding, W Ding
Mediators Inflamm., 2019-08-18;2019(0):2789014.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Increase in HDAC9 suppresses myoblast differentiation via epigenetic regulation of autophagy in hypoxia
Authors: Z Zhang, L Zhang, Y Zhou, L Li, J Zhao, W Qin, Z Jin, W Liu
Cell Death Dis, 2019-07-18;10(8):552.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
RNA Virus-Based Episomal Vector with a Fail-Safe Switch Facilitating Efficient Genetic Modification and Differentiation of iPSCs
Authors: Y Komatsu, D Takeuchi, T Tokunaga, H Sakurai, A Makino, T Honda, Y Ikeda, K Tomonaga
Mol Ther Methods Clin Dev, 2019-05-28;14(0):47-55.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Muscle development and regeneration controlled by AUF1-mediated stage-specific degradation of fate-determining checkpoint mRNAs
Authors: D Abbadi, M Yang, DM Chenette, JJ Andrews, RJ Schneider
Proc. Natl. Acad. Sci. U.S.A., 2019-05-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
IMB0901 inhibits muscle atrophy induced by cancer cachexia through MSTN signaling pathway
Authors: D Liu, X Qiao, Z Ge, Y Shang, Y Li, W Wang, M Chen, S Si, SZ Chen
Skelet Muscle, 2019-03-28;9(1):8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In�Vivo
Authors: LR Fiedler, K Chapman, M Xie, E Maifoshie, M Jenkins, PA Golforoush, M Bellahcene, M Noseda, D Faust, A Jarvis, G Newton, MA Paiva, M Harada, DJ Stuckey, W Song, J Habib, P Narasimham, R Aqil, D Sanmugalin, R Yan, L Pavanello, M Sano, SC Wang, RD Sampson, S Kanayagana, GE Taffet, LH Michael, ML Entman, TH Tan, SE Harding, CMR Low, C Tralau-Ste, T Perrior, MD Schneider
Cell Stem Cell, 2019-03-07;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Epigenetic regulation of the PTEN-AKT-RAC1 axis by G9a is critical for tumor growth in alveolar rhabdomyosarcoma
Authors: AV Bhat, M Palanicham, VK Rao, L Pignata, HJ Lim, S Suriyamurt, KTE Chang, VK Lee, E Guccione, R Taneja
Cancer Res., 2019-03-04;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects
Authors: J Ooi, SR Langley, X Xu, KH Utami, B Sim, Y Huang, NP Harmston, YL Tay, A Ziaei, R Zeng, D Low, F Aminkeng, RM Sobota, F Ginhoux, E Petretto, MA Pouladi
Cell Rep, 2019-02-26;26(9):2494-2508.e7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy
Authors: MC Estañ, E Fernández-, MS Zaki, MI Esteban, S Donkervoor, C Hawkins, JA Caparros-M, D Saade, Y Hu, V Bolduc, KR Chao, J Nevado, A Lamuedra, R Largo, G Herrero-Be, J Regadera, C Hernandez-, EF Tizzano, V Martinez-G, JJ Carvajal, R Zong, DL Nelson, GA Otaify, S Temtamy, M Aglan, M Issa, CG Bönnemann, P Lapunzina, G Yoon, VL Ruiz-Perez
Nat Commun, 2019-02-15;10(1):797.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
p300 mediates muscle wasting in Lewis lung carcinoma
Authors: YP Li, TK Sin, JZ Zhu, G Zhang
Cancer Res., 2019-01-31;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations
Authors: RI Dmitrieva, TA Lelyavina, MY Komarova, VL Galenko, OA Ivanova, PA Tikanova, NV Khromova, AS Golovkin, MA Bortsova, A Sergushich, MY Sitnikova, AA Kostareva
Stem Cells Int, 2019-01-03;2019(0):5690345.
Species: Human
Sample Types: Whole Tissue
Applications: ICC -
Pannexin 1 inhibits rhabdomyosarcoma progression through a mechanism independent of its canonical channel function
Authors: X Xiang, S Langlois, ME St-Pierre, JF Barré, D Grynspan, B Purgina, KN Cowan
Oncogenesis, 2018-11-21;7(11):89.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons
Authors: T Osaki, SGM Uzel, RD Kamm
Sci Adv, 2018-10-10;4(10):eaat5847.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The exercise-inducible bile acid receptor Tgr5 improves skeletal muscle function in mice
Authors: T Sasaki, A Kuboyama, M Mita, S Murata, M Shimizu, J Inoue, K Mori, R Sato
J. Biol. Chem., 2018-05-17;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Activation of the Wnt/ β-catenin pathway by an inflammatory microenvironment affects the myogenic differentiation capacity of human laryngeal mucosa mesenchymal stromal cells
Authors: R Yang, X Yang, S Liu, L Ming, Z Zhou, Y Liang, Y Zhao, F Zhou, ZH Deng, Y Jin
Stem Cells Dev., 2018-05-09;0(0):.
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC-P -
Premyogenic progenitors derived from human pluripotent stem cells expand in floating culture and differentiate into transplantable myogenic progenitors
Authors: F Sakai-Take, A Narita, S Masuda, T Wakamatsu, N Watanabe, T Nishiyama, K Nogami, M Blanc, S Takeda, Y Miyagoe-Su
Sci Rep, 2018-04-26;8(1):6555.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
HSP90 modulates the myosin replacement rate in myofibrils
Authors: K Ojima, E Ichimura, T Suzuki, M Oe, S Muroya, T Nishimura
Am. J. Physiol., Cell Physiol., 2018-03-21;0(0):.
Species: Chicken
Sample Types: Cell Lysates
Applications: Western Blot -
OR2H2 regulates the differentiation of human myoblast cells by its ligand aldehyde 13-13
Authors: B Kalbe, M Osterloh, VM Schulz, J Altmüller, C Becker, S Osterloh, H Hatt
Arch. Biochem. Biophys., 2018-03-17;645(0):72-80.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia
Authors: R Chen, J Xu, Y She, T Jiang, S Zhou, H Shi, C Li
Int. J. Mol. Med., 2018-02-06;41(5):2565-2572.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients
Authors: M Wattin, L Gaweda, P Muller, M Baritaud, C Scholtes, C Lozano, K Gieseler, C Kretz-Remy
Int J Mol Sci, 2018-01-07;19(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration
Authors: M Koide, Y Hagiwara, M Tsuchiya, M Kanzaki, H Hatakeyama, Y Tanaka, T Minowa, T Takemura, A Ando, T Sekiguchi, Y Yabe, E Itoi
Tohoku J. Exp. Med., 2018-01-01;244(1):15-24.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The Major Lysosomal Membrane Proteins LAMP-1 and LAMP-2 Participate in Differentiation of C2C12 Myoblasts
Authors: H Sakane, K Akasaki
Biol. Pharm. Bull., 2018-01-01;41(8):1186-1193.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Canadine from Corydalis turtschaninovii Stimulates Myoblast Differentiation and Protects against Myotube Atrophy
Authors: H Lee, SJ Lee, GU Bae, NI Baek, JH Ryu
Int J Mol Sci, 2017-12-18;18(12):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
Authors: G Zhang, Z Liu, H Ding, Y Zhou, HA Doan, KWT Sin, ZJ Zhu, R Flores, Y Wen, X Gong, Q Liu, YP Li
Nat Commun, 2017-09-19;8(1):589.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
NF?B signaling in alveolar rhabdomyosarcoma
Authors: MM Cleary, A Mansoor, T Settelmeye, Y Ijiri, KJ Ladner, MN Svalina, BP Rubin, DC Guttridge, C Keller
Dis Model Mech, 2017-09-01;10(9):1109-1115.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor
Authors: S Kokabu, C Nakatomi, T Matsubara, Y Ono, WN Addison, JW Lowery, M Urata, AM Hudnall, S Hitomi, M Nakatomi, T Sato, K Osawa, T Yoda, V Rosen, E Jimi
J. Biol. Chem., 2017-06-12;0(0):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Dexamethasone Treatment at the Myoblast Stage Enhanced C2C12 Myocyte Differentiation
Authors: DS Han, WS Yang, TW Kao
Int J Med Sci, 2017-04-08;14(5):434-443.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
R-spondin1 Controls Muscle Cell Fusion through Dual Regulation of Antagonistic Wnt Signaling Pathways
Authors: F Lacour, E Vezin, CF Bentzinger, MC Sincennes, L Giordani, A Ferry, R Mitchell, K Patel, MA Rudnicki, MC Chaboissie, AA Chassot, F Le Grand
Cell Rep, 2017-03-07;18(10):2320-2330.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability
Authors: J Ueki, M Nakamori, M Nakamura, M Nishikawa, Y Yoshida, A Tanaka, A Morizane, M Kamon, T Araki, MP Takahashi, A Watanabe, N Inagaki, H Sakurai
Sci Rep, 2017-02-13;7(0):42522.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
CRISPR screen identifies the NCOR/HDAC3 complex as a major suppressor of differentiation in rhabdomyosarcoma
Proc. Natl. Acad. Sci. U.S.A, 2016-12-12;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Functional loss of DHRS7C induces intracellular Ca2+ overload and myotube enlargement in C2C12 cells via calpain activation
Authors: Hiroyuki Tsutsui
Am. J. Physiol., Cell Physiol., 2016-11-02;0(0):ajpcell.00090.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Activin A induces skeletal muscle catabolism via p38? mitogen-activated protein kinase
Authors: Yi-Ping Li
J Cachexia Sarcopenia Muscle, 2016-09-16;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1
Sci Rep, 2016-08-23;6(0):32084.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Novel Therapeutic Effects of Non-thermal atmospheric pressure plasma for Muscle Regeneration and Differentiation
Sci Rep, 2016-06-28;6(0):28829.
Species: Human, Rat
Sample Types: Protein
Applications: Western Blot -
Citrus unshiu peel extract alleviates cancer-induced weight loss in mice bearing CT-26 adenocarcinoma
Authors: A Kim, M Im, MJ Gu, JY Ma
Sci Rep, 2016-04-11;6(0):24214.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma.
Authors: Vleeshouwer-Neumann T, Phelps M, Bammler T, Macdonald J, Jenkins I, Chen E
PLoS ONE, 2015-12-04;10(12):e0144320.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Mesenchymal Stem Cells from Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project.
Authors: Boyle K, Patinkin Z, Shapiro A, Baker P, Dabelea D, Friedman J
Diabetes, 2015-12-02;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Simple Western -
Molecular ties between the cell cycle and differentiation in embryonic stem cells.
Authors: Li, Victor C, Kirschner, Marc W
Proc Natl Acad Sci U S A, 2014-06-16;111(26):9503-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
Authors: Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V
Am J Pathol, 2014-04-01;184(4):1152-66.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Myoblasts Derived From Normal hESCs and Dystrophic hiPSCs Efficiently Fuse With Existing Muscle Fibers Following Transplantation.
Authors: Goudenege S, Lebel C, Huot N, Dufour C, Fujii I, Gekas J, Rousseau J, Tremblay J
Mol Ther, 2012-09-18;20(11):2153-67.
Species: Human
Sample Types: Whole Cells
Applications: ICC, Western Blot -
Myocilin interacts with syntrophins and is member of dystrophin-associated protein complex.
Authors: Joe MK, Kee C, Tomarev SI
J. Biol. Chem., 2012-02-25;287(16):13216-27.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Slow-dividing satellite cells retain long-term self-renewal ability in adult muscle.
Authors: Ono Y, Masuda S, Nam HS
J. Cell. Sci., 2012-02-20;125(0):1309-17.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Magnolol Attenuates Cisplatin-Induced Muscle Wasting by M2c Macrophage Activation
Authors: Chanju Lee, Hyunju Jeong, Hyunji Lee, Minwoo Hong, Seon-young Park, Hyunsu Bae
Frontiers in Immunology
-
Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis
Authors: Cai R, Zhang Q, Wang Y et al.
The Journal of biological chemistry
-
Interrogating Signaling Nodes Involved in Cellular Transformations Using Kinase Activity Probes
Authors: Cliff I. Stains, Nathan C. Tedford, Traci C. Walkup, Elvedin Luković, Brenda N. Goguen, Linda G. Griffith et al.
Chemistry & Biology
-
CDKAL1 Drives the Maintenance of Cancer Stem-Like Cells by Assembling the eIF4F Translation Initiation Complex
Authors: Huang R, Yamamoto T, Nakata E et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
-
Human iPS Cells Derived Skeletal Muscle Progenitor Cells Promote Myoangiogenesis and Restore Dystrophin in Duchenne Muscular Dystrophic Mice
Authors: Khan M, Ashraf M
Stem Cell Res Ther
-
Active tissue adhesive activates mechanosensors and prevents muscle atrophy
Authors: Sungmin Nam, Bo Ri Seo, Alexander J. Najibi, Stephanie L. McNamara, David J. Mooney
Nature Materials
-
Morroniside ameliorates inflammatory skeletal muscle atrophy via inhibiting canonical and non-canonical NF-kappa B and regulating protein synthesis/degradation
Authors: Xiangjiao Yi, Jianguo Tao, Yu Qian, Feng Feng, Xueqin Hu, Taotao Xu et al.
Frontiers in Pharmacology
-
Cancer-induced muscle wasting requires p38beta MAPK activation of p300.
Authors: Sin, T K, Zhang, G Et al.
Cancer Res
-
Evodiamine promotes differentiation and inhibits proliferation of C2C12 muscle cells
Authors: Xiangping Yao, Taiyong Yu, Chen Zhao, Youlei Li, Ying Peng, Fengxue Xi et al.
International Journal of Molecular Medicine
-
SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1 alpha pathway
Authors: Xiaofan Yang, Pingping Xue, Zhenyu Liu, Wenqing Li, Chuyan Li, Zhenbing Chen
Cellular & Molecular Biology Letters
-
Lcn2 mediates adipocyte-muscle-tumor communication and hypothermia in pancreatic cancer cachexia
Authors: Mengistu Lemecha, Jaya Prakash Chalise, Yuki Takamuku, Guoxiang Zhang, Takahiro Yamakawa, Garrett Larson et al.
Molecular Metabolism
-
Caspase1/11 signaling affects muscle regeneration and recovery following ischemia, and can be modulated by chloroquine
Authors: Ulka Sachdev, Ricardo Ferrari, Xiangdong Cui, Abish Pius, Amrita Sahu, Michael Reynolds et al.
Molecular Medicine
-
Pharmacological Inhibition of HMGB1 Prevents Muscle Wasting
Authors: Lu Li, Huiquan Liu, Weili Tao, Su Wen, Xiaofen Fu, Shiying Yu
Frontiers in Pharmacology
-
Z-ajoene from Crushed Garlic Alleviates Cancer-Induced Skeletal Muscle Atrophy
Authors: Hyejin Lee, Ji-Won Heo, A-Reum Kim, Minson Kweon, Sorim Nam, Jong-Seok Lim et al.
Nutrients
-
Dimethyl-2-oxoglutarate improves redox balance and mitochondrial function in muscle pericytes of individuals with diabetes mellitus
Authors: Ashton Faulkner, Anita Tamiato, William Cathery, Andrea Rampin, Carlo Maria Caravaggi, Eva Jover et al.
Diabetologia
-
Phenotypic Drug Screening for Dysferlinopathy Using Patient-Derived Induced Pluripotent Stem Cells
Authors: Yuko Kokubu, Tomoko Nagino, Katsunori Sasa, Tatsuo Oikawa, Katsuya Miyake, Akiko Kume et al.
Stem Cells Translational Medicine
-
Generation of Functional Myocytes from Equine Induced Pluripotent Stem Cells
Authors: Karin R. Amilon, Yennifer Cortes-Araya, Benjamin Moore, Seungmee Lee, Simon Lillico, Amandine Breton et al.
Cellular Reprogramming
-
Feeder-supported in vitro exercise model using human satellite cells from patients with sporadic inclusion body myositis
Authors: Y Li, W Chen, K Ogawa, M Koide, T Takahashi, Y Hagiwara, E Itoi, T Aizawa, M Tsuchiya, R Izumi, N Suzuki, M Aoki, M Kanzaki
Scientific Reports, 2022-01-20;12(1):1082.
-
Integrated Analyses Reveal Overexpressed Notch1 Promoting Porcine Satellite Cells’ Proliferation through Regulating the Cell Cycle
Authors: Yiren Jiao, Bo Huang, Yu Chen, Guangliang Hong, Jian Xu, Chingyuan Hu et al.
International Journal of Molecular Sciences
-
Mannan Oligosaccharides Promoted Skeletal Muscle Hypertrophy through the Gut Microbiome and Microbial Metabolites in Mice
Authors: Zhao W, Chen L, Tan W et al.
Foods
-
Skeletal muscle releases extracellular vesicles with distinct protein and miRNA signatures that function in the muscle microenvironment
Authors: Watanabe S, Sudo Y, Makino T, Kimura S
PNAS
FAQs
-
Does Myosin Heavy Chain Antibody, Catalog # MAB4470, detect Myosin Heavy Chain in all mammalian species?
Myosin Heavy Chain is a highly conserved protein sequence. Although we have not tested every mammalian species, we expect it will bind to Myosin Heavy Chain in all mammalian species.
Reviews for Myosin Heavy Chain Antibody
Average Rating: 4.7 (Based on 7 Reviews)
Have you used Myosin Heavy Chain Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Immunofluorescence, mouse muscle tissue. On the photo: Myosin Heavy Chain Antibody in red, GAP43 in green.
Heart tissue was fixed with paraformaldehyde and embedded with paraffin. The section was pre-treated with heart induced antigen retrieval and blocked with 10% goat serum and 3% BSA. Then the section was incubated with MAB4470 with a concentration of 10 ug/mL at 4C overnight and incubated with anti-mouse Alexa Fluo 488 secondary antiobody for 1 hour at room temperature. Nuclei were stained with DAPI.