Neuron-specific beta -III Tubulin Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1195G-100
Detection of  beta ‑III Tubulin in A172 cells by Flow Cytometry
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Neuron-specific beta -III Tubulin Alexa Fluor® 488-conjugated Antibody Summary

Specificity
Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # TuJ-1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Rat brain-derived microtubules
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of beta ‑III Tubulin in A172 cells by Flow Cytometry A172 cells were stained with Mouse Anti-Neuron-specific beta ‑III Tubulin Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC1195G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: beta-III Tubulin

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium.

Entrez Gene IDs
10381 (Human)
Alternate Names
Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Neuron-specific beta -III Tubulin Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Glucose oxidation drives trunk neural crest cell development and fate
    Authors: Nekooie Marnany, N;Fodil, R;Féréol, S;Dady, A;Depp, M;Relaix, F;Motterlini, R;Foresti, R;Duband, JL;Dufour, S;
    Journal of cell science
    Species: Avian - Coturnix coturnix japonica (Quail)
    Sample Types: Whole Cells
    Applications: ICC

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