OG 488 BAPTA-1 AM
Chemical Name: N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[4-[[[3',6'-bis(acetyloxy)-2',7'-difluoro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]amino]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester
Purity: ≥90%
Biological Activity
OG 488 BAPTA-1 AM is a cell-permeable, fluorescently labeled Ca2+ indicator (Kd =170 nM). Composed of a green fluorescent probe (OG 488, Cat. No. 6257) conjugated to a cell-permeable Ca2+ chelator (BAPTA AM, Cat. No. 2787). Fluorescent intensity increases approximately 14-fold from resting intensity, upon Ca2+ binding. Displays uniform cellular distribution with minimal subcellular compartmentalization. Excitation/emission λ 494/523 nm.Optical Data for OG 488 BAPTA-1 AM
| Emission Color | Green |
|---|---|
| λabs | 494 nm |
| λem | 523 nm |
| Extinction Coefficient (ε) | 19000 M-1cm-1 |
| Quantum Yield (φ) | 0.91 |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals
Thomas et al.
Cell Calcium, 2000;28:213
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