Porcine IL-1 beta /IL-1F2 Biotinylated Antibody Summary
Ala115-Pro267
Accession # P26889
Applications
Porcine IL-1 beta /IL-1F2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63% - 70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1 beta.
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- Huether, M. et al. (1993) Gene 129:285.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Product Datasheets
Citations for Porcine IL-1 beta /IL-1F2 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
10
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Extracellular ATP does not induce P2X7 receptor-dependent responses in cultured renal- and liver-derived swine macrophages
Authors: Takato Takenouchi, Shunichi Suzuki, Hiroki Shinkai, Mitsutoshi Tsukimoto, Mitsuru Sato, Hirohide Uenishi et al.
Results in Immunology
-
Plasma Cytokines and Birth Weight as Biomarkers of Vaccine-Induced Humoral Responses in Piglets
Authors: Sean Lipsit, Antonio Facciuolo, Erin Scruten, Philip Griebel, Scott Napper
Frontiers in Veterinary Science
-
Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network Dispersion
Authors: KP Pandey, Y Zhou
Viruses, 2022-01-05;14(1):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Detection -
Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes
Authors: DE Marin, CV Bulgaru, CA Anghel, GC Pistol, MI Dore, ML Palade, I Taranu
Toxins, 2020-12-15;12(12):.
Species: Porcine
Sample Types: Tissue Homogenates
Applications: ELISA Detection -
Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines
Authors: SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella
MethodsX, 2019-05-17;6(0):1218-1227.
Species: Porcine
Sample Types: Tissue Homogenates
Applications: Detection Antibody -
Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production.
Authors: Park H, Liu G, Liu Q, Zhou Y
Viruses, 2018-08-09;10(8):.
Species: Porcine
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: ELISA Development, Western Blot -
NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination
Authors: HS Park, G Liu, SN Thulasi Ra, SL Landreth, Q Liu, Y Zhou
J. Virol., 2018-03-28;0(0):.
Species: Porcine
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: Western Blot, ELISA Detection, ELISA Development -
Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
Cytometry A, 2006-05-01;69(5):391-5.
Species: Porcine
Sample Types: Serum
Applications: Luminex Development -
NLRP3 Inflammasome Activation Enhanced by TRIM25 is Targeted by the NS1 Protein of 2009 Pandemic Influenza A Virus
Authors: Hong-Su Park, Yao Lu, Kannupriya Pandey, GuanQun Liu, Yan Zhou
Frontiers in Microbiology
-
The NS1 Protein of Influenza A Virus Participates in Necroptosis by Interacting with MLKL and Increasing Its Oligomerization and Membrane Translocation
Authors: Amit Gaba, Fang Xu, Yao Lu, Hong-Su Park, GuanQun Liu, Yan Zhou
Journal of Virology
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Staining Reagents
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Porcine IL-1 beta /IL-1F2 Biotinylated Antibody
Average Rating: 5 (Based on 3 Reviews)
Have you used Porcine IL-1 beta /IL-1F2 Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
