Porcine IL-8/CXCL8 Antibody Summary
Ala26-Gln104
Accession # CAA43461
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine CXCL8/IL‑8 Antibody. Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535). The ND50 is typically 1.5-7.5 µg/mL.
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Detection of Porcine CXCL8/IL-8 by Western Blot Inflammatory response in PAMs induced by PRRSV 5′UTR RNA and LPS. A, B PAMs were transfected with different doses of 5′UTR RNA (1, 2, and 4 μg/well) along with 1 μg/mL LPS. qRT-PCR and Western blot results showed that compared with PAMs in other groups, PAMs in the 4 μg 5′UTR RNA and 1 μg/mL LPS co-stimulation group produced higher levels of IL-1 beta (p < 0.05). C After co-stimulation, the relative expression level of IL-1 beta mRNA obtained was similar at the 12 and 24 h time-points. D 5′UTR RNA and LPS co-stimulation induced IL-1 beta expression in cells and supernatants. E, F 5′UTR RNA and LPS co-stimulation induced increased levels of IL-6, IL-8 and TNF-alpha mRNA and protein. Expression was normalized to that of GAPDH. Different letters (a, b, c, d, and e) on data indicate significant differences between groups (p < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31300043), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Porcine CXCL8/IL-8 by Western Blot Inflammatory response in PAMs induced by PRRSV 5′UTR RNA and LPS. A, B PAMs were transfected with different doses of 5′UTR RNA (1, 2, and 4 μg/well) along with 1 μg/mL LPS. qRT-PCR and Western blot results showed that compared with PAMs in other groups, PAMs in the 4 μg 5′UTR RNA and 1 μg/mL LPS co-stimulation group produced higher levels of IL-1 beta (p < 0.05). C After co-stimulation, the relative expression level of IL-1 beta mRNA obtained was similar at the 12 and 24 h time-points. D 5′UTR RNA and LPS co-stimulation induced IL-1 beta expression in cells and supernatants. E, F 5′UTR RNA and LPS co-stimulation induced increased levels of IL-6, IL-8 and TNF-alpha mRNA and protein. Expression was normalized to that of GAPDH. Different letters (a, b, c, d, and e) on data indicate significant differences between groups (p < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31300043), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-8/CXCL8
Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.
IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro-inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivo and in vitro.
- Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.
Product Datasheets
Citations for Porcine IL-8/CXCL8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
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G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer
Authors: Maija Hollmén, Sinem Karaman, Simon Schwager, Angela Lisibach, Ailsa J. Christiansen, Mikael Maksimow et al.
OncoImmunology
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Matrine inhibits IL-1? secretion in primary porcine alveolar macrophages through the MyD88/NF-κB pathway and NLRP3 inflammasome
Authors: P Sun, N Sun, W Yin, Y Sun, K Fan, J Guo, A Khan, Y He, H Li
Vet. Res., 2019-07-12;50(1):53.
Species: Porcine
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Exposure to hog barn dust alters airway epithelial ciliary beating.
Authors: Wyatt TA, Sisson JH, Von Essen SG, Poole JA, Romberger DJ
Eur. Respir. J., 2008-01-23;31(6):1249-55.
Species: Porcine
Sample Types: Complex Sample Type
Applications: Western Blot
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After labeling with Sulfo-Tag, used as a detection reagent in MSD assay (Meso Scale Diagnostics LLC). Paired with biotinylated IL-8 antibody (MAB535) as a capture reagent. A standard curve with recombinant porcine IL-8 from R&D (Cat# 535-IN/CF) is shown (2-25,000 pg/ml).
The antibody MAB5351 was used as the capture antibody in a sandwich ELISA for Porcine IL-8 along with the detection antibody, AF535. The calibrator for the assay was recombinant porcine IL-8, 535-IN. It was sensitive and specific.
