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Porcine TNF-alpha Antibody

Catalog #: MAB6902
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6902
MAB6902-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine TNF-alpha Antibody Summary

Species Reactivity
Porcine
Specificity
Detects porcine TNF-alpha  in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 103304
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant porcine TNF-alpha
Arg78-Leu232
Accession # P23563
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Porcine TNF-alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Porcine TNF‑ alpha Biotinylated Antibody (Catalog # BAM6903)

Standard: Recombinant Porcine TNF-alpha Protein (Catalog # 690-PT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Porcine TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 176 aa extracellular domain (ECD) (3). Within the ECD, porcine TNF-alpha shares 69% - 86% aa sequence identity with bovine, canine, cotton rat, equine, feline, human, mouse, rat, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).

References
  1. Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. 
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. 
  3. Pauli, U. et al. (1989) Gene 81:185.
  4. Tang, P. et al. (1996) Biochemistry 35:8216. 
  5. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. 
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  9. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335.
  10. Romanatto, T. et al. (2007) Peptides 28:1050.
  11. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.
Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

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Citations for Porcine TNF-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
    Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E
    Intensive Care Med, 2007-04-25;33(6):1085-93.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  2. Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
    Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
    Cytometry A, 2006-05-01;69(5):391-5.
    Species: Porcine
    Sample Types: Serum
    Applications: Luminex Development
  3. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
    Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
    J. Virol., 2006-01-01;80(1):372-82.
    Species: Porcine
    Sample Types: Serum
    Applications: ELISA Development
  4. omega -3 PUFA Rich Camelina Oil By-Products Improve the Systemic Metabolism and Spleen Cell Functions in Fattening Pigs
    Authors: Ionelia Taranu, Mihail Gras, Gina Cecilia Pistol, Monica Motiu, Daniela E. Marin, Nicoleta Lefter et al.
    PLoS ONE

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Porcine TNF-alpha Antibody
By Anonymous on 11/08/2017
Application: ELISA Sample Tested: Porcine Serum,Serum and Plasma Species: Porcine

The antibody MAB6902 was used as the capture antibody paired withe detection antibody BAM6903 in a sandwich assay for Porcine TNFa. The calibrator was 690-PT. The assay had a sensitivity of 2 pg/ml.

ELISA Porcine TNF-alpha Antibody MAB6902