Primary Mouse Cortical Stem Cells

Mouse Cortical Stem Cells (Catalog # NSC002)

Ex vivo expanded neural stem cells serve as excellent tools for researchers studying neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 CD-1 mice. These cells are designated as passage 1 (P1) cells. Cells were cultured as a monolayer, passaged once, and the expanded progeny cryopreserved. Every lot of R&D Systems Mouse Cortical Stem Cells is tested for a high level of Nestin expression and the capacity for multi-lineage differentiation (astrocytes, neurons, and oligodendrocytes).

Our rigorous testing procedures ensure the highest level of quality and consistency from lot to lot. Depending on your research needs, our Mouse Cortical Stem Cells can be optimally expanded as monolayers or neurospheres. In addition, all mouse cortical stem cell lots tested negative for mycoplasma using the MycoProbe™ Mycoplasma Detection Kit (Catalog # CUL001B). The cells also tested negative for microbial contamination.

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Mouse Cortical Stem Cells. A, Undifferentiated mouse cortical stem cells cultured on poly-L-ornithine and fibronectin coated dishes. B - D, Mouse cortical stem cells differentiated for 7 days. Neuronal lineage cells (B) were detected with Mouse Anti-Neuron Specific beta III Tubulin (Catalog # MAB1195) followed by NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG (Catalog # NL007). Astrocyte lineage cells (C) were detected with Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by NorthernLights 557-conjugated Donkey Anti-Sheep IgG (Catalog # NL010). Oligodendrocyte lineage cells (D) were detected with Mouse Anti-Oligodendrocyte Marker O4 (Catalog # MAB1326). Image A is shown at 100X. Images B, C, and D are shown at 200X.