ProDots Recombinant Human EGF Protein
ProDots Recombinant Human EGF Protein Summary
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
ProDots® proteins are R&D Systems industry-leading quality, highly bioactive proteins packaged into easy-to-use lyophilized “dots”. Designed to be stable at 4 ºC and to dissolve instantly, ProDots® proteins can be rolled into cell culture media to quickly produce media with a desired working protein concentration.
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
The ED50 for this effect is 20-100 pg/mL.
Asn971 - Arg1023, with an N-terminal Met
Analysis
6 kDa, reducing conditions
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
PRD236
| Reconstitution | For a stock solution, reconstitute at 500 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Scientific Data
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ProDots®Recombinant Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line.The ED50 for this effect is 20-100 pg/mL.
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1 μg/lane of ProDots®Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) conditions and visualized with silver staining, showing a single band at 6 kDa.
Reconstitution Calculator
Background: EGF
EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.
Product Specific Notices
726219.1Citations for ProDots Recombinant Human EGF Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Growth factor-induced activation of MSK2 leads to phosphorylation of H3K9me2S10 and corresponding changes in gene expression
Authors: Wong, KG;Cheng, YF;Wu, VH;Kiseleva, AA;Li, J;Poleshko, A;Smith, CL;Epstein, JA;
Science advances
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression
Authors: L Ma, Y Tian, T Qian, W Li, C Liu, B Chu, Q Kong, R Cai, P Bai, L Ma, Y Deng, R Tian, C Wu, Y Sun
Cell Death & Disease, 2022-05-20;13(5):482.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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