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Proteome Profiler Human Protease Array Kit

Catalog #: ARY021B
29 Citations 1 Review Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 35 different protease antibodies
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ARY021B
R&D Systems Proteome Profiler Antibody Array Kits
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Citations (29)
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Proteome Profiler Human Protease Array Kit Summary

Kit Summary

To simultaneously detect the relative changes of 35 proteases in a single sample. No specialized equipment is necessary

 

Troubleshooting Guide
 

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, plasma, urine, saliva, or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the Product Datasheet.

Stability and Storage

Store the unopened product at 2° C to 8° C. Do not use past expiration date.

 

Simultaneously detect the relative levels of these proteases in a single sample.
ADAM8 Cathepsin X/Z/P MMP-7
ADAM9 DPPIV/CD26 MMP-8
ADAMTS1 Kallikrein 3/PSA MMP-9
ADAMTS13 Kallikrein 5 MMP-10
Cathepsin A Kallikrein 6 MMP-12
Cathepsin B Kallikrein 7 MMP-13
Cathepsin C/DPPI Kallikrein 10 Neprilysin/CD10
Cathepsin D Kallikrein 11 Presenilin-1
Cathepsin E Kallikrein 13 Proprotein Convertase 9
Cathepsin L MMP-1 Proteinase 3
Cathepsin S MMP-2 uPA/Urokinase
Cathepsin V MMP-3  

Assays for Analytes represented in the Human Protease Array Kit

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
ADAM8   DY1031
ADAM9   DY939
ADAMTS1   DY2197
ADAMTS13 DADT130  
Cathepsin A    
Cathepsin B DCATB0 DY953, DY2176
Cathepsin C/DPPI    
Cathepsin D    
Cathepsin E    
Cathepsin L   DY952
Cathepsin S   DY1183, DY2227
Cathepsin V   DY1080
Cathepsin X/Z/P    
DPPIV/CD26   DY1180
Kallikrein 3/PSA DKK300 DY1344
Kallikrein 5 DKK500  
Kallikrein 6    
Kallikrein 7    
Kallikrein 10    
Kallikrein 11    
Kallikrein 13    
MMP-1 DMP100  
MMP-2 MMP200 DY902
MMP-3 DMP300 DY513
MMP-7 DMP700 DY907
MMP-8 DMP800 DY908
MMP-9 DMP900 DY911
MMP-10 DM1000 DY910
MMP-12    
MMP-13 DM1300 DY511, DY913
Neprilysin   DY1182
Presenilin-1   DY149
Proprotein Convertase 9 DPC900 DY3888
Proteinase 3   DY6134-05
uPA/Urokinase DUPA00 DY1310

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

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Citations for Proteome Profiler Human Protease Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Pyrocurzerenone suppresses human oral cancer cell metastasis by inhibiting the expression of ERK1/2 and cathepsin S proteins
    Authors: Hsieh, MJ;Lin, CC;Ho, HY;Chuang, YC;Lo, YS;Chen, MK;
    Journal of cellular and molecular medicine  2024-08-01
  2. Tofacitinib Regulates Endostatin via Effects on CD147 and Cathepsin S
    Authors: Zisman, D;Sabtan, H;Rahat, MM;Simanovich, E;Haddad, A;Gazitt, T;Feld, J;Slobodin, G;Kibari, A;Elias, M;Rahat, MA;
    International journal of molecular sciences  2024-07-02
  3. Epiberberine suppresses the metastasis of head and neck squamous cell carcinoma cells by regulating the MMP-13 and JNK pathway
    Authors: Ho, HY;Chen, MK;Lin, CC;Lo, YS;Chuang, YC;Hsieh, MJ;
    Journal of cellular and molecular medicine  2023-09-14
  4. Ectopic expression of DOCK8 regulates lysosome-mediated pancreatic tumor cell invasion
    Authors: Gutierrez-Ruiz, OL;Johnson, KM;Krueger, EW;Nooren, RE;Cruz-Reyes, N;Heppelmann, CJ;Hogenson, TL;Fernandez-Zapico, ME;McNiven, MA;Razidlo, GL;
    Cell reports  2023-08-30
  5. Multiscale stiffness of human emphysematous precision cut lung slices
    Authors: Kim, JH;Schaible, N;Hall, JK;Bartol�k-Suki, E;Deng, Y;Herrmann, J;Sonnenberg, A;Behrsing, HP;Lutchen, KR;Krishnan, R;Suki, B;
    Science advances  2023-05-19
  6. Galectin-3 promotes secretion of proteases that decrease epithelium integrity in human colon cancer cells
    Authors: S Li, DM Pritchard, LG Yu
    Cell Death & Disease, 2023-04-13;14(4):268.  2023-04-13
  7. Overexpressed Malat1 Drives Metastasis through Inflammatory Reprogramming of Lung Adenocarcinoma Microenvironment
    Authors: E Mart Nez-T, FJ de Miguel, V Li, C Robles-Ote, K Politi, JR Zamudio, N Dimitrova
    bioRxiv : the preprint server for biology, 2023-03-23;0(0):.  2023-03-23
  8. Microphysiological model of PIK3CA-driven vascular malformations reveals a role of dysregulated Rac1 and mTORC1/2 in lesion formation
    Authors: WY Aw, C Cho, H Wang, AH Cooper, EL Doherty, D Rocco, SA Huang, S Kubik, CP Whitworth, R Armstrong, AJ Hickey, B Griffith, ML Kutys, J Blatt, WJ Polacheck
    Science Advances, 2023-02-15;9(7):eade8939.  2023-02-15
  9. Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of Orthobiologics
    Authors: C Giannasi, L Mangiavini, S Niada, A Colombo, E Della Mort, V Vismara, A Ambrosanio, P Savadori, S Casati, GM Peretti, AT Brini
    Pharmaceutics, 2022-06-10;14(6):.  2022-06-10
  10. Decidual macrophage derived MMP3 contributes to extracellular matrix breakdown in spiral artery remodeling in early human pregnancy
    Authors: Y Pan, L Yang, D Chen, H Hou, M Zhang, M Chen, F Ning, Q Lu, M Zhao, L Li, GE Lash
    Journal of reproductive immunology, 2022-02-11;150(0):103494.  2022-02-11
  11. Anti-Periodontitis Effect of Ethanol Extracts of Alpinia Katsumadai Seeds
    Authors: SW Shin, YS Hwang
    Nutrients, 2021-12-28;14(1):.  2021-12-28
  12. Kallikrein-Related Peptidase 6 Is Associated with the Tumour Microenvironment of Pancreatic Ductal Adenocarcinoma
    Authors: JB Candido, O Maiques, M Boxberg, V Kast, E Peerani, E Tomás-Bort, W Weichert, A Sananes, N Papo, V Magdolen, V Sanz-Moren, D Loessner
    Cancers, 2021-08-05;13(16):.  2021-08-05
  13. Pinosylvin inhibits migration and invasion of nasopharyngeal carcinoma cancer cells via regulation of epithelial?mesenchymal transition and inhibition of MMP?2
    Authors: YC Chuang, MC Hsieh, CC Lin, YS Lo, HY Ho, MJ Hsieh, JT Lin
    Oncology reports, 2021-06-03;46(1):.  2021-06-03
  14. Gingival Crevicular Fluid Zinc- and Aspartyl-Binding Protease Profile of Individuals with Moderate/Severe Atopic Dermatitis
    Authors: F Valenzuela, J Fernández, M Aroca, C Jiménez, D Albers, M Hernández, A Fernández
    Biomolecules, 2020-11-26;10(12):.  2020-11-26
  15. Cancer-associated fibroblast stimulates cancer cell invasion in an interleukin-1 receptor (IL-1R)-dependent manner
    Authors: X Zhang, YS Hwang
    Oncol Lett, 2019-08-27;18(5):4645-4650.  2019-08-27
  16. S100A8 & S100A9: Alarmin mediated inflammation in tendinopathy
    Authors: LAN Crowe, M McLean, SM Kitson, EG Melchor, K Patommel, HM Cao, JH Reilly, WJ Leach, BP Rooney, SJ Spencer, M Mullen, M Chambers, GAC Murrell, IB McInnes, M Akbar, NL Millar
    Sci Rep, 2019-02-06;9(1):1463.  2019-02-06
  17. Anti-invasive and Anti-tumor Effects of Dryopteris crassirhizoma Extract by Disturbing Actin Polymerization
    Authors: J Lee, YH Nho, SK Yun, YS Hwang
    Integr Cancer Ther, 2019-01-01;18(0):1534735419851.  2019-01-01
  18. Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis
    Authors: MR Blatchley, F Hall, S Wang, HC Pruitt, S Gerecht
    Sci Adv, 2019;5(3):eaau7518.  2019
  19. Stromal mesenchymal stem cells facilitate pancreatic cancer progression by regulating specific secretory molecules through mutual cellular interaction
    Authors: K Saito, M Sakaguchi, S Maruyama, H Iioka, EW Putranto, IW Sumardika, N Tomonobu, T Kawasaki, K Homma, E Kondo
    J Cancer, 2018-07-30;9(16):2916-2929.  2018-07-30
  20. Differential Sensitivity of Human Hepatocellular Carcinoma Xenografts to an IGF-II Neutralizing Antibody May Involve Activated STAT3
    Authors: SA Greenall, J Donoghue, TG Johns, TE Adams
    Transl Oncol, 2018-06-22;11(4):971-978.  2018-06-22
  21. Intranasal delivery of VEGF enhances compensatory lung growth in mice
    Authors: DT Dao, JT Vuong, L Anez-Busti, A Pan, PD Mitchell, GL Fell, MA Baker, DR Bielenberg, M Puder
    PLoS ONE, 2018-06-07;13(6):e0198700.  2018-06-07
  22. Astrocytic tight junctions control inflammatory CNS lesion pathogenesis
    Authors: S Horng, A Therattil, S Moyon, A Gordon, K Kim, AT Argaw, Y Hara, JN Mariani, S Sawai, P Flodby, ED Crandall, Z Borok, MV Sofroniew, C Chapouly, GR John
    J. Clin. Invest., 2017-07-24;0(0):.  2017-07-24
  23. HIV Reprograms Human Airway Basal Stem/Progenitor Cells to Acquire a Tissue-Destructive Phenotype
    Authors: NPY Chung, X Ou, KMF Khan, J Salit, RJ Kaner, RG Crystal
    Cell Rep, 2017-05-09;19(6):1091-1100.  2017-05-09
  24. Inhibition of cathepsin S confers sensitivity to methyl protodioscin in oral cancer cells via activation of p38 MAPK/JNK signaling pathways
    Authors: MJ Hsieh, CW Lin, MK Chen, SY Chien, YS Lo, YC Chuang, YT Hsi, CC Lin, JC Chen, SF Yang
    Sci Rep, 2017-03-22;7(0):45039.  2017-03-22
  25. Inhibition of radiation-induced glioblastoma invasion by genetic and pharmacological targeting of MDA-9/Syntenin
    Proc. Natl. Acad. Sci. U.S.A, 2016-12-23;0(0):.  2016-12-23
  26. Blockade of voltage-gated sodium channels inhibits invasion of endocrine-resistant breast cancer cells.
    Authors: Mohammed F, Khajah M, Yang M, Brackenbury W, Luqmani Y
    Int J Oncol, 2015-11-09;48(1):73-83.  2015-11-09
  27. Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis.
    Authors: Andrade B, Pavan Kumar N, Amaral E, Riteau N, Mayer-Barber K, Tosh K, Maier N, Conceicao E, Kubler A, Sridhar R, Banurekha V, Jawahar M, Barbosa T, Manganiello V, Moss J, Fontana J, Marciano B, Sampaio E, Olivier K, Holland S, Jackson S, Moayeri M, Leppla S, Sereti I, Barber D, Nutman T, Babu S, Sher A
    J Immunol, 2015-08-12;195(6):2763-73.  2015-08-12
  28. Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening.
    Authors: Kumar D, Moore R, Nash A, Springel E, Mercer B, Philipson E, Mansour J, Moore J
    Placenta, 2014-10-12;35(12):1049-56.  2014-10-12
  29. Shedding of dipeptidyl peptidase 4 is mediated by metalloproteases and up-regulated by hypoxia in human adipocytes and smooth muscle cells.
    Authors: Rohrborn D, Eckel J, Sell H
    FEBS Lett, 2014-09-12;588(21):3870-7.  2014-09-12

FAQs

  1. Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    • We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

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Proteome Profiler Human Protease Array Kit
By Gabriele Strusi on 09/17/2024