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Human, Mouse, & Rat Total MMP-2 ELISA Kit - Quantikine

Catalog #: MMP200
101 Citations 5 Reviews Datasheet / COA / SDS
For human, mouse, rat, porcine, and canine samples
Catalog # Availability Size / Price Qty
MMP200
PMMP200
SMMP200
Control Products Available
QC126: Quantikine Immunoassay Control Set 923 for Total MMP-2
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Human/Mouse/Rat/Porcine/Canine Total MMP-2 ELISA Standard Curve
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Citations (101)
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Reviews (5)
Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Total MMP-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.082 ng/mL
Assay Range
0.5 - 32 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Recombinant MMP-2 and natural human, mouse, rat, porcine, and canine active, pro-, and TIMP complexed MMP-2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Total MMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure MMP-2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse/rat pro-MMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural MMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MMP-2.

Recovery

The recovery of Total MMP-2 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 90-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total MMP-2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human/Mouse/Rat/Porcine/Canine Total MMP-2 ELISA Linearity

Scientific Data

N/A Human/Mouse/Rat/Porcine/Canine Total MMP-2 ELISA Standard Curve View Larger

Human/Mouse/Rat/Porcine/Canine Total MMP-2 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-2

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 2
Entrez Gene IDs:
4313 (Human); 17390 (Mouse)
Alternate Names:
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Total MMP-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

101 Citations: Showing 1 - 10
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  1. Doxazosin inhibits vasculogenic mimicry in human non?small cell lung cancer through inhibition of the VEGF?A/VE?cadherin/mTOR/MMP pathway
    Authors: Hsu, JL;Leu, WJ;Hsu, LC;Hsieh, CH;Guh, JH;
    Oncology letters
    Species: Human
    Sample Types: Cell Culture Supernates
  2. In Vivo Study on Doxycycline Protective Mechanisms during Myocardial Ischemia Injury in Rats
    Authors: Krzywonos-Zawadzka, A;Olejnik, A;Sawicki, G;Bil-Lula, I;
    Biomedicines
    Species: Rat
    Sample Types: Tissue Homogenates
  3. Dapagliflozin Improved Cardiac Function and Structure in Diabetic Patients with Preserved Ejection Fraction: Results of a Single Centre, Observational Prospective Study
    Authors: Cortés, M;Lorenzo, O;Lumpuy-Castillo, J;Martínez-Albaladejo, S;Taibo-Urquía, M;Pello, AM;Bollas, AJ;Orejas, M;Navas, MÁ;Macia, E;Martínez, ME;Rueda, A;Tuñón, J;
    Journal of clinical medicine
    Species: Human
    Sample Types: Plasma, Serum, Urine
  4. TNF? induces matrix metalloproteinase-9 expression in monocytic cells through ACSL1/JNK/ERK/NF-kB signaling pathways
    Authors: Al-Roub, A;Akhter, N;Al-Rashed, F;Wilson, A;Alzaid, F;Al-Mulla, F;Sindhu, S;Ahmad, R;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Analysis of MMP-2-735C/T (rs2285053) and MMP-9-1562C/T (rs3918242) Polymorphisms in the Risk Assessment of Developing Lung Cancer
    Authors: Wadowska, K;Błasiak, P;Rzechonek, A;Śliwińska-Mossoń, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  6. LRP1 in vascular mural cells modulates cerebrovascular integrity and function in the presence of APOE4
    Authors: H Oue, Y Yamazaki, W Qiao, C Yuanxin, Y Ren, A Kurti, F Shue, TM Parsons, RB Perkerson, K Kawatani, N Wang, SC Starling, B Roy, IE Mosneag, T Aikawa, ML Holm, CC Liu, Y Inoue, PM Sullivan, YW Asmann, BY Kim, G Bu, T Kanekiyo
    JCI Insight, 2023-04-10;8(7):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. The Significance of Matrix Metalloproteinase 9 (MMP-9) and Metalloproteinase 2 (MMP-2) in Urinary Bladder Cancer
    Authors: J Kudelski, A Tokarzewic, M Gudowska-S, B Mroczko, P Ch?osta, M Bruczko-Go, P Mitura, G M?ynarczyk
    Biomedicines, 2023-03-20;11(3):.
    Species: Human
    Sample Types: Tissue Homogenates
  8. Analysis of Selected Salivary Adipokines and Cytokines in Patients with Obesity-A Pilot Study
    Authors: L Ostrowska, J Smarkusz-Z, A Gornowicz, K Lendzion, B Zy?k, D Pogodzi?sk
    International Journal of Molecular Sciences, 2023-02-18;24(4):.
    Species: Human
    Sample Types: Saliva
  9. Pro-Inflammatory Adipokine and Cytokine Profiles in the Saliva of Obese Patients with Non-Alcoholic Fatty Liver Disease (NAFLD)-A Pilot Study
    Authors: B Zy?k, L Ostrowska, J Smarkusz-Z, K Witczak-Sa, A Gornowicz, A Bielawska
    International Journal of Molecular Sciences, 2023-02-02;24(3):.
    Species: Human
    Sample Types: Saliva
  10. Punica granatum L. Polyphenolic Extract as an Antioxidant to Prevent Kidney Injury in Metabolic Syndrome Rats
    Authors: A Radajewska, J Szyller, J Niewiadoms, A Noszczyk-N, I Bil-Lula
    Oxidative Medicine and Cellular Longevity, 2023-01-05;2023(0):6144967.
    Species: Rat
    Sample Types: Tissue Homogenates
  11. Enzymes of Fibrosis in Chronic Liver Disease
    Authors: I Tsomidis, G Notas, C Xidakis, A Voumvourak, DN Samonakis, M Koulentaki, E Kouroumali
    Biomedicines, 2022-12-08;10(12):.
    Species: Human
    Sample Types: Serum
  12. Factors Associated with RANTES, EMMPIRIN, MMP2 and MMP9, and the Association of These Biomarkers with Cardiovascular Disease in a Multi-Ethnic Population
    Authors: LY Wang, CS Tan, MKP Lai, S Hilal
    Journal of Clinical Medicine, 2022-12-08;11(24):.
    Species: Human
    Sample Types: Serum
  13. Klotho Protein Decreases MMP-Mediated Degradation of Contractile Proteins during Ischaemia/Reperfusion Injury to the Cardiomyocytes
    Authors: A Olejnik, A Krzywonos-, M Banaszkiew, I Bil-Lula
    International Journal of Molecular Sciences, 2022-12-07;23(24):.
    Species: Human
    Sample Types: Cell Lysates
  14. Expression of Matrix Metalloproteinase-2,-7,-9 in Serum during Pregnancy in Patients with Pre-Eclampsia: A Prospective Study
    Authors: A Bahabayi, N Yang, T Xu, Y Xue, L Ma, X Gu, Y Wang, K Jia
    International Journal of Environmental Research and Public Health, 2022-11-04;19(21):.
    Species: Human
    Sample Types: Serum
  15. Circulating biomarker correlates of left atrial size and myocardial extracellular volume fraction among persons living with and without HIV
    Authors: TE Peterson, C Landon, SA Haberlen, F Bhondoekha, MW Plankey, FJ Palella, DA Piggott, JB Margolick, TT Brown, WS Post, KC Wu
    BMC cardiovascular disorders, 2022-09-03;22(1):393.
    Species: Human
    Sample Types: Serum
  16. Angiogenin and MMP-2 as potential biomarkers in the differential diagnosis of gestational trophoblastic diseases
    Authors: D Weng, T Han, J Dong, M Zhang, Y Mi, Y He, X Li, X Zhu
    Medicine, 2022-02-04;101(5):e28768.
    Species: Human
    Sample Types: Serum
  17. AKT-AMPKalpha-mTOR-dependent HIF-1alpha Activation is a New Therapeutic Target for Cancer Treatment: A Novel Approach to Repositioning the Antidiabetic Drug Sitagliptin for the Management of Hepatocellular Carcinoma
    Authors: EE Abd El-Fat, S Saber, ME Youssef, H Eissa, E El-Ahwany, NA Amin, M Alqarni, GE Batiha, AJ Obaidullah, MMY Kaddah, AG Ahmed Gaaf, AAE Mourad, G Mostafa-He, AM Abdelhamid
    Frontiers in Pharmacology, 2022-01-12;12(0):720173.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Plasmatic MMP9 released from tumor-infiltrating neutrophils is predictive for bevacizumab efficacy in glioblastoma patients: an AVAglio ancillary study
    Authors: C Jiguet-Jig, S Boissonnea, E Denicolai, V Hein, R Lasseur, J Garcia, S Romain, R Appay, T Graillon, W Mason, AF Carpentier, AA Brandes, L' Ouafik, W Wick, A Baaziz, JP Gigan, RJ Argüello, D Figarella-, O Chinot, E Tabouret
    Acta neuropathologica communications, 2022-01-03;10(1):1.
    Species: Human
    Sample Types: Plasma
  19. CD147 Levels in Blood and Adipose Tissues Correlate with Vascular Dysfunction in Obese Diabetic Adults
    Authors: MM Ali, I Mirza, D Naquiallah, C Hassan, M Masrur, FM Bianco, AM Mahmoud
    Journal of cardiovascular development and disease, 2021-12-28;9(1):.
    Species: Human
    Sample Types: Plasma
  20. Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation
    Authors: D Bakkalci, A Jay, A Rezaei, CA Howard, HJ Haugen, J Pape, S Kishida, M Kishida, G Jell, TR Arnett, S Fedele, U Cheema
    Scientific Reports, 2021-12-16;11(1):24088.
    Species: Human, Rat
    Sample Types: Cell Culture Supernates
  21. Differential Modulation of Matrix Metalloproteinases-2 and -7 in LAM/TSC Cells
    Authors: S Ancona, E Orpianesi, C Bernardell, E Chiaramont, R Chiaramont, S Terraneo, F Di Marco, E Lesma
    Biomedicines, 2021-11-24;9(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Novel electrogravimetric biosensors for the ultrasensitive detection of plasma matrix metalloproteinase-2 considered a potential tumor biomarker
    Authors: MK Nisiewicz, A Gajda, A Kowalczyk, A Cupriak, A Kasprzak, M Bamburowic, IP Grudzinski, AM Nowicka
    Analytica chimica acta, 2021-11-19;1191(0):339290.
    Species: Rat
    Sample Types: Plasma
  23. BMP7 reduces the fibrocartilage chondrocyte phenotype
    Authors: EGJ Ripmeester, MMJ Caron, GGH van den Ak, J Steijns, DAM Surtel, A Cremers, LCW Peeters, LW van Rhijn, TJM Welting
    Scientific Reports, 2021-10-04;11(1):19663.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Expression of atrial?fetal light chains in cultured human cardiomyocytes after chemical ischemia?reperfusion injury
    Authors: M Banaszkiew, A Olejnik, A Krzywonos-, K Ha?ucha, I Bil-Lula
    Molecular Medicine Reports, 2021-09-07;24(5):.
    Species: Human
    Sample Types: Cell Lysates
  25. ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier
    Authors: X Yue, Y Qian, L Zhu, B Gim, M Bao, J Jia, S Jing, Y Wang, C Tan, F Bottanelli, P Ziltener, S Choi, P Hao, I Lee
    Bmc Biology, 2021-09-07;19(1):194.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. New Insights on Old Biomarkers Involved in Tumor Microenvironment Changes and Their Diagnostic Relevance in Non-Small Cell Lung Carcinoma
    Authors: K Wadowska, P B?asiak, A Rzechonek, I Bil-Lula, M ?liwi?ska-
    Biomolecules, 2021-08-13;11(8):.
    Species: Human
    Sample Types: Serum
  27. In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and In Vivo Analysis of Its Therapeutic Potential in Osteoarthritis
    Authors: SE Huang, E Sulistyowa, YY Chao, BN Wu, ZK Dai, JH Hsu, JL Yeh
    Biomedicines, 2021-05-28;9(6):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. Endurance Training Depletes Antioxidant System but Does Not Affect Endothelial Functions in Women with Abdominal Obesity: A Randomized Trial with a Comparison to Endurance-Strength Training
    Authors: M Jamka, P Bogda?ski, P Krzy?anows, A Mi?kiewicz, J Karolkiewi, M Du?-?uchow, R M?dry, A Lisowska, A Gotz-Wi?ck, S Iskakova, J Walkowiak, E M?dry
    Journal of Clinical Medicine, 2021-04-12;10(8):.
    Species: Human
    Sample Types: Whole Blood
  29. The antihypertensive agent hydralazine reduced extracellular matrix synthesis and liver fibrosis in nonalcoholic steatohepatitis exacerbated by hypertension
    Authors: Y Yuan, H Naito, K Kitamori, S Hashimoto, T Asano, T Nakajima
    PLoS ONE, 2020-12-14;15(12):e0243846.
    Species: Rat
    Sample Types: Serum
  30. Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model
    Authors: Q Liang, Q Lin, Y Li, W Luo, X Huang, Y Jiang, C Qin, J Nong, X Chen, SR Sooranna, L Pinhu
    Journal of Immunology Research, 2020-11-25;2020(0):6644687.
    Species: Rat
    Sample Types: Serum
  31. Matrix Metalloproteinases MMP-2 and MMP-9, Their Inhibitors TIMP-1 and TIMP-2, Vascular Endothelial Growth Factor and sVEGFR-2 as Predictive Markers of Ischemic Retinopathy in Patients with Systemic Sclerosis-Case Series Report
    Authors: A Waszczykow, M Podgórski, M Waszczykow, Z Gerlicz-Ko, P Jurowski
    Int J Mol Sci, 2020-11-18;21(22):.
    Species: Human
    Sample Types: Serum
  32. Prognosis of metastasis based on age and serum analytes after follow-up of non-metastatic lung cancer patients
    Authors: MMS Balla, S Patwardhan, PK Melwani, P Purwar, A Kumar, CS Pramesh, S Laskar, BN Pandey
    Transl Oncol, 2020-11-11;14(1):100933.
    Species: Human
    Sample Types: Serum
  33. Tissue Expression of Atrial and Ventricular Myosin Light Chains in the Mechanism of Adaptation to Oxidative Stress
    Authors: M Banaszkiew, A Krzywonos-, A Olejnik, I Bil-Lula
    Int J Mol Sci, 2020-11-09;21(21):.
    Species: Rat
    Sample Types: Tissue Homogenates
  34. Anti-Cancer Activity of As4O6 and its Efficacy in a Series of Patient-Derived Xenografts for Human Cervical Cancer
    Authors: JJ Noh, MS Kim, YJ Cho, SY Jeong, YY Lee, JY Ryu, JJ Choi, I Bae, Z Wu, BG Kim, JR Hwang, JW Lee
    Pharmaceutics, 2020-10-19;12(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Aberrant recruitment of leukocytes defines poor wound healing in patients with recessive dystrophic epidermolysis bullosa
    Authors: T Phillips, L Huitema, R Cepeda, DL Cobos, RIM Perez, MS Garza, F Ringpfeil, B Dasgeb, J Uitto, JC Salas-Alan, V Alexeev, O Igoucheva
    J Dermatol Sci, 2020-10-17;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Anti-Melanogenic Effects of Korean Red Ginseng Oil in an Ultraviolet B-Induced Hairless Mouse Model
    Authors: E Saba, SH Kim, YY Lee, HK Kim, SS Roh, YS Kwak, CK Park, SD Kim, MH Rhee
    Molecules, 2020-10-16;25(20):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  37. Cdc42-Dependent Transfer of mir301 from Breast Cancer-Derived Extracellular Vesicles Regulates the Matrix Modulating Ability of Astrocytes at the Blood-Brain Barrier
    Authors: G Morad, CC Daisy, HH Otu, TA Libermann, ST Dillon, MA Moses
    Int J Mol Sci, 2020-05-28;21(11):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  38. Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA
    Authors: YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li
    Chin. Med. J., 2020-03-20;0(6):679-686.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-&beta1 release system
    Authors: T Nii, K Makino, Y Tabata
    Regen Ther, 2020-03-04;14(0):196-204.
    Species: Human
    Sample Types: Cell Cultue Supernates
  40. First Insights into the Effect of Low-Dose X-Ray Irradiation in Adipose-Derived Stem Cells
    Authors: A Schröder, S Kriesen, G Hildebrand, K Manda
    Int J Mol Sci, 2019-12-02;20(23):.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Development of Kaempferol-Loaded Gelatin Nanoparticles for the Treatment of Corneal Neovascularization in Mice
    Authors: YL Chuang, HW Fang, A Ajitsaria, KH Chen, CY Su, GS Liu, CL Tseng
    Pharmaceutics, 2019-11-28;11(12):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  42. Evaluation of NV556, a Novel Cyclophilin Inhibitor, as a Potential Antifibrotic Compound for Liver Fibrosis
    Authors: S Simón Serr, A Grönberg, L Longato, K Rombouts, J Kuo, M Gregory, S Moss, E Elmér, G Mazza, P Gallay, M Pinzani, MJ Hansson, R Massoumi
    Cells, 2019-11-08;8(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Levels of Selected Matrix Metalloproteinases, Their Inhibitors in Saliva, and Oral Status in Juvenile Idiopathic Arthritis Patients vs. Healthy Controls
    Authors: A Kobus, J Bagi?ska, J ?api?ska-A, S ?awicki, A Kierklo
    Biomed Res Int, 2019-10-28;2019(0):7420345.
    Species: Human
    Sample Types: Saliva
  44. miR-27a regulates vascular remodeling by targeting endothelial cells' apoptosis and interaction with vascular smooth muscle cells in aortic dissection
    Authors: Y Sun, Y Xiao, H Sun, Z Zhao, J Zhu, L Zhang, J Dong, T Han, Q Jing, J Zhou, Z Jing
    Theranostics, 2019-10-18;9(25):7961-7975.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts
    Authors: M Aoki, K Koga, M Miyazaki, M Hamasaki, N Koshikawa, M Oyama, H Kozuka-Hat, M Seiki, BP Toole, K Nabeshima
    BMC Cancer, 2019-09-12;19(1):912.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1
    Authors: DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei
    Front Neurosci, 2019-08-14;13(0):836.
    Species: Human
    Sample Types: Plasma
  47. Effect of doxycyline in chronic obstructive pulmonary disease - An exploratory study
    Authors: B Singh, N Ghosh, D Saha, S Sarkar, P Bhattachar, K Chaudhury
    Pulm Pharmacol Ther, 2019-07-24;0(0):101831.
    Species: Human
    Sample Types: Plasma
  48. Effect of Peritoneal Dialysis on Serum Fibrosis Biomarkers in Patients with Refractory Congestive Heart Failure
    Authors: M Kunin, V Carmon, P Beckerman, D Dinour
    Int J Mol Sci, 2019-05-28;20(11):.
    Species: Human
    Sample Types: Plasma
  49. Longitudinal Changes of PAI-1, MMP-2, and VEGF in Peritoneal Effluents and Their Associations with Peritoneal Small-Solute Transfer Rate in New Peritoneal Dialysis Patients
    Authors: N Hao, TT Chiou, CH Wu, YY Lei, PL Liang, MC Chao, H Yang, JB Chen
    Biomed Res Int, 2019-05-05;2019(0):2152584.
    Species: Human
    Sample Types: Peritoneal Fluid
  50. Highly glycosylated CD147 promotes hemorrhagic transformation after rt-PA treatment in diabetes: a novel therapeutic target?
    Authors: Y Xie, Y Wang, H Ding, M Guo, X Wang, Q Dong, M Cui
    J Neuroinflammation, 2019-04-05;16(1):72.
    Species: Mouse
    Sample Types: Serum
  51. Inflammatory cytokines in highly myopic eyes
    Authors: J Yuan, S Wu, Y Wang, S Pan, P Wang, L Cheng
    Sci Rep, 2019-03-05;9(1):3517.
    Species: Human
    Sample Types: Aqueous Humor
  52. Liposomal Curcumin is Better than Curcumin to Alleviate Complications in Experimental Diabetic Mellitus
    Authors: AE Bulboac?, AS Porfire, LR Tefas, PM Boarescu, SD Bolboac?, IC St?nescu, AC Bulboac?, G Dogaru
    Molecules, 2019-02-27;24(5):.
    Species: Rat
    Sample Types: Plasma
  53. A potential key mechanism in ascending aortic aneurysm development: Detection of a linear relationship between MMP-14/TIMP-2 ratio and active MMP-2
    Authors: R Schmitt, A Tscheuschl, P Laschinski, X Uffelmann, P Discher, J Fuchs, M Kreibich, R Peyronnet, FA Kari
    PLoS ONE, 2019-02-22;14(2):e0212859.
    Species: Human
    Sample Types: Tissue Homogenates
  54. Cardioprotective effect of MMP-2-inhibitor-NO-donor hybrid against ischaemia/reperfusion injury
    Authors: A Krzywonos-, A Franczak, A Olejnik, M Radomski, JF Gilmer, G Sawicki, M Wo?niak, I Bil-Lula
    J. Cell. Mol. Med., 2019-02-07;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  55. Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters, and Serological Worsening Markers in Nonalcoholic Fatty Liver Disease Patients
    Authors: A Federico, M Dallio, M Masarone, AG Gravina, R Di Sarno, C Tuccillo, V Cossiga, S Lama, P Stiuso, F Morisco, M Persico, C Loguercio
    Oxid Med Cell Longev, 2019;2019(0):8742075.
    Species: Human
    Sample Types: Serum
  56. Changes in brain white matter structure are associated with urine proteins in urologic chronic pelvic pain syndrome (UCPPS): A MAPP Network study
    Authors: DC Woodworth, A Dagher, A Curatolo, M Sachdev, C Ashe-McNal, BD Naliboff, JS Labus, JR Landis, JJ Kutch, EA Mayer, RS Lee, MA Moses, BM Ellingson
    PLoS ONE, 2018-12-05;13(12):e0206807.
    Species: Human
    Sample Types: Urine
  57. Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
    Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
    J Clin Med, 2018-11-08;7(11):.
    Species: Human
    Sample Types: Plasma
  58. Elevated Inflammatory Plasma Biomarkers in Patients With Fabry Disease: A Critical Link to Heart Failure With Preserved Ejection Fraction
    Authors: H Yogasundar, A Nikhanj, BN Putko, M Boutin, S Jain-Ghai, A Khan, C Auray-Blai, ML West, GY Oudit
    J Am Heart Assoc, 2018-11-06;7(21):e009098.
    Species: Human
    Sample Types: Plasma
  59. Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/??catenin pathway
    Authors: S Zeng, B Zhu, J Zeng, W Wu, C Jiang
    Mol Med Rep, 2018-10-17;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. Matrix metalloproteinase promotes elastic fiber degradation in ligamentum flavum degeneration
    Authors: K Sugimoto, T Nakamura, T Tokunaga, Y Uehara, T Okada, T Taniwaki, T Fujimoto, H Mizuta
    PLoS ONE, 2018-08-01;13(8):e0200872.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  61. Ginkgo biloba extracts prevent aortic rupture in angiotensin II-infused hypercholesterolemic mice
    Authors: XF Huang, SZ Zhang, YY You, N Zhang, H Lu, A Daugherty, XJ Xie
    Acta Pharmacol. Sin., 2018-05-18;0(0):.
    Species: Mouse
    Sample Types: Serum
  62. Specific biomarkers of myocardial inflammation and remodeling processes as predictors of mortality in high-risk patients undergoing percutaneous mitral valve repair (MitraClip)
    Authors: O Dörr, C Walther, C Liebetrau, T Keller, H Tabert, N Boeder, M Bayer, P Bauer, H Möllmann, L Gaede, C Troidl, S Voss, T Bauer, CW Hamm, H Nef
    Clin Cardiol, 2018-04-17;0(0):.
    Species: Human
    Sample Types: Serum
  63. Matrix Metalloproteinase-Mediated Blood-Brain Barrier Dysfunction in Epilepsy
    Authors: RG Rempe, AMS Hartz, ELB Soldner, BS Sokola, SR Alluri, EL Abner, RJ Kryscio, A Pekcec, J Schlichtig, B Bauer
    J. Neurosci., 2018-04-09;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  64. Cardiac effects of fish oil in a rat model of streptozotocin-induced diabetes
    Authors: F Mayyas, R Jaradat, KH Alzoubi
    Nutr Metab Cardiovasc Dis, 2018-02-25;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  65. Nesfatin-1 and visfatin expression is associated with reduced atherosclerotic disease risk in patients with rheumatoid arthritis
    Authors: C Robinson, L Tsang, A Solomon, AJ Woodiwiss, S Gunter, M Mer, HC Hsu, M Gomes, GR Norton, AME Millen, PH Dessein
    Peptides, 2018-02-21;102(0):31-37.
    Species: Human
    Sample Types: Plasma
  66. miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development
    Authors: J Ma, D Li, FF Kong, D Yang, H Yang, XX Ma
    J. Exp. Clin. Cancer Res., 2018-02-01;37(1):19.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. Activity of metalloproteinases and adiponectin in obese patients-a possible factor of incisional hernias after bariatric procedures
    Authors: W Szcz?sny, M Kuligowska, S D?browieck, J Szmytkowsk, A Re?li?ski, M S?upski
    J Zhejiang Univ Sci B, 2018-01-01;19(1):65-70.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  68. CCAAT/enhancer-binding protein ? overexpression alleviates myocardial remodelling by regulating angiotensin-converting enzyme-2 expression in diabetes
    Authors: Y Tie, C Zhai, Y Zhang, X Qin, F Yu, H Li, M Shan, C Zhang
    J. Cell. Mol. Med., 2017-12-21;0(0):.
    Species: Mouse
    Sample Types: Serum
  69. Recombinant human IL-26 facilitates the innate immune response to endotoxin in the bronchoalveolar space of mice in vivo
    Authors: A Bao, KF Che, S Bozinovski, J Ji, JA Gregory, S Kumlien Ge, M Adner, LO Cardell, A Lindén
    PLoS ONE, 2017-12-05;12(12):e0188909.
    Species: Mouse
    Sample Types: BALF
  70. Down-regulating Myoferlin inhibits the vasculogenic mimicry of�melanoma via decreasing MMP-2 and inducing mesenchymal-to-epithelial transition
    Authors: W Zhang, P Zhou, A Meng, R Zhang, Y Zhou
    J. Cell. Mol. Med., 2017-11-22;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  71. Epigallocatechin gallate & curcumin prevent transforming growth factor beta 1-induced epithelial to mesenchymal transition in ARPE-19 cells
    Authors: S Shanmugana, VN Sumantran, N Angayarkan
    Indian J. Med. Res., 2017-11-01;146(0):S85-S96.
    Species: Human
    Sample Types: Cell Culture Supernates
  72. Does cardiac resynchronization therapy restore peripheral circulatory homeostasis?
    Authors: CN Ajaero, CR Chong, NEK Procter, S Liu, YY Chirkov, T Heresztyn, WPA Chan, MA Arstall, AD McGavigan, MP Frenneaux, JD Horowitz
    ESC Heart Fail, 2017-10-13;5(1):129-138.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  73. Chebulinic acid inhibits smooth muscle cell migration by suppressing PDGF-R? phosphorylation and inhibiting matrix metalloproteinase-2 expression
    Authors: IS Song, YJ Jeong, JH Park, S Shim, SW Jang
    Sci Rep, 2017-09-18;7(1):11797.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Suppressive Effect of the n-Hexane Extract of Litsea japonica Fruit Flesh on Monosodium-Iodoacetate-Induced Osteoarthritis in Rats
    Authors: SH Kim, HJ Choi, WK Yang, JE Lee, JH Cho, IJ Park, S Park, BK Park, M Jin
    Evid Based Complement Alternat Med, 2017-08-22;2017(0):1791403.
    Species: Rat
    Sample Types: Tissue Homogenates
  75. Beneficial Effects of Galectin-3 Blockade in Vascular and Aortic Valve Alterations in an Experimental Pressure Overload Model
    Authors: J Ibarrola, E Martínez-M, JR Sádaba, V Arrieta, A García-Peñ, V Álvarez, A Fernández-, A Gainza, P Rossignol, V Cachofeiro, N López-Andr
    Int J Mol Sci, 2017-07-31;18(8):.
    Species: Rat
    Sample Types: Tissue Homogenates
  76. Circulating tumor cells and serum levels of MMP-2, MMP-9 and VEGF as markers of the metastatic process in patients with high risk of metastatic progression
    Authors: M Skerenova, V Mikulova, O Capoun, T Zima, P Tesarova
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 2017-05-16;0(0):.
    Species: Human
    Sample Types: Serum
  77. Comparison of fibrin glue and Vicryl sutures in conjunctival autografting for pterygium surgery
    Authors: X Wang, Y Zhang, L Zhou, R Wei, L Dong
    Mol. Vis., 2017-04-19;23(0):275-285.
    Species: Human
    Sample Types: Tears
  78. TIMP1 and MMP9 are predictors of mortality in septic patients in the emergency department and intensive care unit unlike MMP9/TIMP1 ratio: Multivariate model
    Authors: ME Niño, SE Serrano, DC Niño, DM McCosham, ME Cardenas, VP Villareal, M Lopez, A Pazin-Filh, FA Jaimes, F Cunha, R Schulz, D Torres-Due
    PLoS ONE, 2017-02-13;12(2):e0171191.
    Species: Human
    Sample Types: Serum
  79. Aurora Kinase A is a Biomarker for Bladder Cancer Detection and Contributes to its Aggressive Behavior
    Authors: A Mobley, S Zhang, J Bondaruk, Y Wang, T Majewski, NP Caraway, L Huang, E Shoshan, G Velazquez-, G Nitti, S Lee, JG Lee, E Fuentes-Ma, D Willis, L Zhang, CC Guo, H Yao, K Baggerly, Y Lotan, SP Lerner, C Dinney, D McConkey, M Bar-Eli, B Czerniak
    Sci Rep, 2017-01-19;7(0):40714.
    Species: Human
    Sample Types: Cell Culture Supernates
  80. A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease
    Authors: Ilias P Doulamis
    Int. J. Cardiol, 2016-12-23;0(0):.
    Species: Human
    Sample Types: Serum
  81. Concomitant elevations of MMP-9, NGAL, proMMP-9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease
    J. Cell. Mol. Med, 2016-12-22;0(0):.
    Species: Human
    Sample Types: Serum
  82. Differential diagnosis of lung cancer, its metastasis and chronic obstructive pulmonary disease based on serum Vegf, Il-8 and MMP-9
    Sci Rep, 2016-11-04;6(0):36065.
    Species: Human
    Sample Types: Serum
  83. MMP-2 Isoforms in Aortic Tissue and Serum of Patients with Ascending Aortic Aneurysms and Aortic Root Aneurysms
    PLoS ONE, 2016-11-01;11(11):e0164308.
    Species: Human
    Sample Types: Serum
  84. The pattern of hMENA isoforms is regulated by TGF-?1 in pancreatic cancer and may predict patient outcome
    Authors: R Melchionna, P Iapicca, F Di Modugno, P Trono, I Sperduti, M Fassan, I Cataldo, BC Rusev, RT Lawlor, MG Diodoro, M Milella, GL Grazi, MJ Bissell, A Scarpa, P Nisticò
    Oncoimmunology, 2016-08-12;5(12):e1221556.
    Species: Human
    Sample Types: Cell Culture Supernates
  85. CP-25, a novel compound, protects against autoimmune arthritis by modulating immune mediators of inflammation and bone damage
    Sci Rep, 2016-05-17;6(0):26239.
    Species: Rat
    Sample Types: Synovial Fluid
  86. Exosomes in Human Breast Milk Promote EMT
    Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter
    Clin Cancer Res, 2016-04-08;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  87. Magnobovatol inhibits smooth muscle cell migration by suppressing PDGF-R? phosphorylation and inhibiting matrix metalloproteinase-2 expression
    Authors: H Kang, DH Ahn, JH Pak, KH Seo, NI Baek, SW Jang
    Int J Mol Med, 2016-04-04;37(5):1239-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  88. Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.
    Authors: Kmiecik A, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski M, Podhorska-Okolow M, Dziegiel P
    PLoS ONE, 2015-05-01;10(5):e0124865.
    Species: Human
    Sample Types: Cell Culture Supernates
  89. Aqueous and alcoholic extracts of Triphala and their active compounds chebulagic acid and chebulinic acid prevented epithelial to mesenchymal transition in retinal pigment epithelial cells, by inhibiting SMAD-3 phosphorylation.
    Authors: Sivasankar S, Lavanya R, Brindha P, Angayarkanni N
    PLoS ONE, 2015-03-20;10(3):e0120512.
    Species: Human
    Sample Types: Cell Culture Supernates
  90. WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
    Authors: Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K
    Mol Cancer, 2014-04-26;13(0):88.
    Species: Human
    Sample Types: Cell Culture Supernates
  91. Oxidative and pro-inflammatory impact of regular and denicotinized cigarettes on blood brain barrier endothelial cells: is smoking reduced or nicotine-free products really safe?
    Authors: Naik, Pooja, Fofaria, Neel, Prasad, Shikha, Sajja, Ravi K, Weksler, Babette, Couraud, Pierre-O, Romero, Ignacio, Cucullo, Luca
    BMC Neurosci, 2014-04-23;15(0):51.
    Species: Human
    Sample Types: Cell Culture Supernates
  92. Overexpression of EMMPRIN isoform 2 is associated with head and neck cancer metastasis.
    Authors: Huang Z, Tan N, Guo W, Wang L, Li H, Zhang T, Liu X, Xu Q, Li J, Guo Z
    PLoS ONE, 2014-04-04;9(4):e91596.
    Species: Human
    Sample Types: Cell Culture Supernates
  93. Decreased PGE(2) content reduces MMP-1 activity and consequently increases collagen density in human varicose vein.
    Authors: Gomez I, Benyahia C, Louedec L, Leseche G, Jacob M, Longrois D, Norel X
    PLoS ONE, 2014-02-05;9(2):e88021.
  94. Role of serum VEGFA, TIMP2, MMP2 and MMP9 in monitoring response to adjuvant radiochemotherapy in patients with primary cervical cancer--results of a companion protocol of the randomized NOGGO-AGO phase III clinical trial.
    Authors: Braicu E, Gasimli K, Richter R, Nassir M, Kummel S, Blohmer J, Yalcinkaya I, Chekerov R, Ignat I, Ionescu A, Mentze M, Fotopoulou C, Pop C, Lichtenegger W, Sehouli J
    Anticancer Res, 2014-01-01;34(1):385-91.
    Species: Human
    Sample Types: Serum
  95. Effect of mesenchymal stem cells and extracts derived from the placenta on trophoblast invasion and immune responses.
    Authors: Choi, Jong Ho, Jung, Jieun, Na, Kyu-Hwan, Cho, Kyung Ji, Yoon, Tae Ki, Kim, Gi Jin
    Stem Cells Dev, 2013-10-19;23(2):132-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  96. Identification of FHL1 as a therapeutic target for Duchenne muscular dystrophy.
    Authors: D'Arcy, Colleen, Feeney, Sandra J, McLean, Catriona, Gehrig, Stefan M, Lynch, Gordon S, Smith, Jaclyn E, Cowling, Belinda, Mitchell, Christin, McGrath, Meagan J
    Hum Mol Genet, 2013-09-18;23(3):618-36.
    Species: Human
    Sample Types: Whole Cells
  97. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013-04-15;190(10):5329-36.
    Species: Mouse
    Sample Types: Tissue Homogenates
  98. Tissue redox activity as a hallmark of carcinogenesis: from early to terminal stages of cancer.
    Authors: Bakalova R, Zhelev Z, Aoki I, Saga T
    Clin Cancer Res, 2013-03-26;19(9):2503-17.
    Species: Mouse
    Sample Types: Plasma
  99. Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy.
    Authors: Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J
    Cytokine, 2013-02-14;61(3):755-8.
    Species: Human
    Sample Types: Serum
  100. Neutrophil-derived matrix metalloproteinase-9 is a potent activator of trypsinogen in acinar cells in acute pancreatitis.
    Authors: Awla D, Abdulla A, Syk I, Jeppsson B, Regner S, Thorlacius H
    J. Leukoc. Biol., 2011-11-18;91(5):711-9.
    Species: Mouse
    Sample Types: Plasma
  101. Celastrol suppresses allergen-induced airway inflammation in a mouse allergic asthma model.
    Authors: Kim DY, Park JW, Jeoung D, Ro JY
    Eur. J. Pharmacol., 2009-04-05;612(1):98-105.
    Species: Mouse
    Sample Types: BALF

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Total MMP-2 Quantikine ELISA Kit
By Anonymous on 03/16/2023
Sample Tested: EDTA Plasma,Urine

Tested plasma at 30-fold dilution and all samples were in range. Tested urine at 2-fold dilution and all samples were undetectable.

Total MMP-2 Quantikine ELISA Kit MMP200
Total MMP-2 Quantikine ELISA Kit
By Anonymous on 09/26/2022
Sample Tested: Serum
Total MMP-2 Quantikine ELISA Kit MMP200
Total MMP-2 Quantikine ELISA Kit
By Anonymous on 04/06/2022
Sample Tested: Human Plasma
Total MMP-2 Quantikine ELISA Kit MMP200
Total MMP-2 Quantikine ELISA Kit
By Anonymous on 10/26/2021
Sample Tested: 7TD1 mouse hybridoma cell line
Total MMP-2 Quantikine ELISA Kit MMP200
Total MMP-2 Quantikine ELISA Kit
By Anonymous on 03/21/2019
Sample Tested: Human Aortic Endothelial Cells