Rat beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

Catalog #: MAB6698 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6698
MAB6698-SP

beta ‑1,3-Glucuronyltransferase 1/B3GAT1 in Rat Cortical Stem Cells.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 in ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 882302

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat beta ‑1,3‑Glucuronyltransferase 1/B3GAT1
Asp75-Ile347
Accession # NP_445455

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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beta ‑1,3-Glucuronyltransferase 1/B3GAT1 in Rat Cortical Stem Cells. beta -1,3-Glucuronyltransferase 1/B3GAT1 was detected in immersion fixed differentiated rat cortical stem cells using Mouse Anti-Rat beta -1,3-Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB6698) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Oligo2 was also detected in the cells using Human Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Cells were counterstained with DAPI (blue). Specific staining of B3GAT1 was localized to transmembrane Golgi. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

B3GAT1 is a key enzyme involved in human natural killer-1 (HNK-1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 1-4GlcNAc-) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK-1 epitope, a unique trisaccharide structure, HSO₃-3GlcA beta 1-3Gal beta 1-4GlcNAc- (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3).The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell‑cell and cell‑substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N‑terminal cytoplasmic domain and a single‑pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

References

Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093.
Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711.
Kakuda, S. et al. (2005) Glycobiology 2:203.
Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77.
Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase

Entrez Gene IDs

27087 (Human); 76898 (Mouse); 117108 (Rat)

Alternate Names

B3GAT1; beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); Beta-1,3-glucuronyltransferase 1; CD57; EC 2.4.1.135; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GLCATP; GlcAT-P; GLCATPGLCUATP; GLCUATP; glcUAT-P; Glucuronosyltransferase P; HNK1; LEU7; NK1; NK-1; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase