Rat beta 2-Microglobulin Antibody

Catalog #: MAB3864 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3864
MAB3864-SP

Detection of Rat beta 2‑Microglobulin in Rat Splenocytes.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat beta 2-Microglobulin Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat beta 2-Microglobulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat alpha 2-Macroglobulin is observed.

Source

Monoclonal Mouse IgM Clone # 747502

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat beta 2-Microglobulin
Ile21-Met119
Accession # P07151

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of Rat beta 2-Microglobulin antibody in Rat Splenocytes.

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Detection of Rat beta 2‑Microglobulin in Rat Splenocytes. Rat splenocytes were stained with Anti-Rat CD3 FITC-conjugated Monoclonal Antibody and either (A) Mouse IgM Isotype control or (B) Mouse Anti-Rat beta 2-Microglobulin Monoclonal Antibody (Catalog # MAB3864) followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta 2-Microglobulin

beta ₂-Microglobulin ( beta ₂M) is a ubiquitous, 12 kDa, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat beta ₂M is a 99 amino acid (aa) peptide containing one C1-type lg-like domain (aa 22-116). In humans, beta ₂M is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91, and 90 amino acids. Mature rat beta ₂M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively.

Entrez Gene IDs

567 (Human); 12010 (Mouse); 24223 (Rat); 101867173 (Cynomolgus Monkey)

Alternate Names

B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin