Rat CCR9 Antibody

Catalog #: MAB8287 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8287
MAB8287-SP

Detection of CCR9 in HEK293 Human Cell Line Transfected with Rat CCR9 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CCR9 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CCR9 in direct ELISAs. Stains rat CCR9 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 882416

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CCR9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR9 in HEK293 Human Cell Line Transfected with Rat CCR9 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) rat CCR9 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CCR9 Monoclonal Antibody (Catalog # MAB8287) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR9

CCR9 is an approximately 42 kDa (predicted) seven transmembrane domain receptor for the chemokine CCL25/TECK. It is expressed on CD8⁺ T cells, monocytes, dendritic cells, and IgA⁺ plasma cells. It plays a role in oral and gut tolerance, the development of g/d T cells, and the thymic recruitment of hematopoietic progenitor cells. CCR9⁺ cells limit inflammatory cell infiltration in rheumatoid arthritis, hepatitis, colitis, and inflammatory bowel disease, but exacerbate it in atherosclerosis. In the brain, CCR9 is expressed on CA1 region pyramidal neurons in the hippocampus and is upregulated on microglia during T. gondii infection. CCR9 is also upregulated in a variety of cancers where it promotes tumor cell metastasis. Rat CCR9 shares 86% and 98% amino acid sequence identity with human and mouse CCR9, respectively.

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Alternate Names

C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28