Rat CD25/IL-2R alpha Antibody

Catalog #: MAB51561 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB51561
MAB51561-SP

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Rat CD25/IL-2R alpha Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CD25/IL-2 R alpha in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) CD25/IL-2 R alpha, rmIL-2 R beta, rmCommon gamma chain, rmIL-15 R alpha, or recombinant human CD25/IL-2 R alpha is observed.

Source

Monoclonal Mouse IgG₁ Clone # 745520

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant rat CD25/IL-2 R alpha
Glu22-Gln235
Accession # P26897

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Rat splenocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma _c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1 - 3). The rat IL-2 R alpha cDNA encodes a 267 amino acid (aa) precursor that includes a 21 aa signal peptide, a 214 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4). Within the ECD, rat IL-2 R alpha shares 58% and 81% aa sequence identity with human and mouse IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma _c. IL-2 R beta (CD122) and gamma _c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma _c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4⁺CD25⁺ Treg which suppress autoreactive CD4⁺ T cells, thereby contributing to peripheral T cell homeostasis (11-13).

References

Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
Page, T.H. and M.J. Dallman (1991) Eur. J. Immunol. 21:2133.
Hatakeyama, M. et al. (1989) Science 244:551.
Takeshita, T. et al. (1992) Science 257:379.
Wang, X. et al. (2005) Science 310:1159.
Wagner, D.K. et al. (1986) J. Immunol. 137:592.
Schulz, O. et al. (1998) J. Exp. Med. 187:271.
Witkowska, A.M. (2005) Mediators Inflamm. 2005:121.
Willerford, D.M. et al. (1995) Immunity 3:521.
Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Long Name

Interleukin 2 Receptor alpha

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Alternate Names

CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR