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Rat IL-1 beta /IL-1F2 Antibody

Catalog #: MAB5011
6 Citations Datasheet / COA / SDS
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MAB5011-SP
MAB5011-100
MAB5011-500

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Detection of Rat IL-1 beta /IL-1F2 by Western Blot
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Citations (6)
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Rat IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IL-1 beta in direct ELISAs and Western blots. In these formats, this antibody shows approximately 100% cross‑reactivity with recombinant mouse (rm) IL-1 beta and rcrIL-1 beta  and no cross-reactivity with rfIL‑1 beta, rhIL-1 beta, or rrmIL-1 beta.
Source
Monoclonal Mouse IgG1 Clone # 38123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat IL-1 beta
Val117-Ser268
Accession # Q63264
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat IL-1 beta /IL-1F2 by Western Blot View Larger

Detection of Rat IL-1 beta /IL-1F2 by Western Blot Plantaris protein content results for interleukin-1 beta (IL-1 beta ) (A). Data were normalized to CON. Values are mean ± SEM (CON: n = 4; SNL: n = 4; 100CUR: n = 4; 50CMO: n = 4). Φ p < 0.050 vs. ipsilateral leg. The representative blot images display IL-1 beta and alpha -tubulin for CON, SNL, 100CUR, and 50CMO groups (B). MW, molecular weight (kDa). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1467-3045/46/11/742), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Citations for Rat IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Huoxue Qianyang Qutan recipe attenuates cardiac fibrosis by inhibiting the NLRP3 inflammasome signalling pathway in obese hypertensive rats
    Authors: Bo Lu, Jun Xie, Deyu Fu, Xiaozhe Chen, Mingyi Zhao, Mingtai Gui et al.
    Pharmaceutical Biology
  2. Metformin induces pyroptosis in leptin receptor-defective hepatocytes via overactivation of the AMPK axis
    Authors: B Liu, J Xu, L Lu, L Gao, S Zhu, Y Sui, T Cao, T Yang
    Cell Death & Disease, 2023-02-03;14(2):82.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. AMPK activation attenuates inflammatory pain through inhibiting NF-?B activation and IL-1? expression
    Authors: HC Xiang, LX Lin, XF Hu, H Zhu, HP Li, RY Zhang, L Hu, WT Liu, YL Zhao, Y Shu, HL Pan, M Li
    J Neuroinflammation, 2019-02-12;16(1):34.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Mitochondria Protection after Acute Ischemia Prevents Prolonged Upregulation of IL-1? and IL-18 and Arrests CKD
    Authors: HH Szeto, S Liu, Y Soong, SV Seshan, L Cohen-Goul, V Manichev, LC Feldman, T Gustafsson
    J. Am. Soc. Nephrol., 2016-11-23;28(5):1437-1449.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Cinnamaldehyde and allopurinol reduce fructose-induced cardiac inflammation and fibrosis by attenuating CD36-mediated TLR4/6-IRAK4/1 signaling to suppress NLRP3 inflammasome activation
    Authors: Lin-Lin Kang
    Sci Rep, 2016;6(0):27460.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
    BMC Neurosci, 2012-05-23;13(0):50.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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