Home / MCAM/CD146 / Rat MCAM/CD146 PE-conjugated Antibody

Rat MCAM/CD146 PE-conjugated Antibody

Catalog #: FAB3250P
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3250P-100UG
Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry.
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Citations (3)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat MCAM/CD146 PE-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) MCAM, rhBCAM, or rhALCAM is observed.
Source
Monoclonal Mouse IgG2A Clone # 404722
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
Arg19-Lys560
Accession # Q9EPF2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MCAM/CD146 antibody in PC-12 Rat Cell Line antibody by Flow Cytometry. View Larger

Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat MCAM/CD146 PE-conjugated Monoclonal Antibody (Catalog # FAB3250P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCAM/CD146

Melanoma Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is an Ig-superfamily adhesion molecule that is expressed on endothelial cells, neurons and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively.

Long Name
Melanoma Cell Adhesion Molecule
Entrez Gene IDs
4162 (Human); 84004 (Mouse); 78967 (Rat)
Alternate Names
CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin

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Citations for Rat MCAM/CD146 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Engineering Functional Vasculature in Decellularized Lungs Depends on Comprehensive Endothelial Cell Tropism
    Authors: Ifeolu Akinnola, Daniel R. Rossi, Carolyn Meyer, Ashley Lindsey, Douglas R. Haase, Samuel Fogas et al.
    Frontiers in Bioengineering and Biotechnology
  2. Nicotinamide mononucleotide (NMN) supplementation rescues cerebromicrovascular endothelial function and neurovascular coupling responses and improves cognitive function in aged mice
    Authors: S Tarantini, MN Valcarcel-, P Toth, A Yabluchans, Z Tucsek, T Kiss, P Hertelendy, M Kinter, P Ballabh, Z Süle, E Farkas, JA Baur, DA Sinclair, A Csiszar, Z Ungvari
    Redox Biol, 2019-04-10;24(0):101192.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Systemic administration of erythropoietin inhibits retinopathy in RCS rats.
    Authors: Shen W, Chung S, Irhimeh M, Li S, Lee S, Gillies M
    PLoS ONE, 2014-08-13;9(8):e104759.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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