Rat SMAGP Antibody

Catalog #: AF3967 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3967
AF3967-SP

All SMAGP Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat SMAGP Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat SMAGP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human SMAGP is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat SMAGP
Met1-Thr34, Phe56-Ile97
Accession # Q7TPF1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat SMAGP

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of rat pancreas

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMAGP

SMAGP (small transmembrane and glycosylated protein) is a 25 kDa, type III transmembrane glycoprotein that participates in epithelial cell polarity. Rat SMAGP is 97 amino acids (aa) in length and contains no signal sequence. The N-terminal 34 amino acids constitute the extracellular domain and show O-linked glycosylation. The C‑terminal 42 amino acids (56‑97) make up the cytoplasmic region. SMAGP is likely involved in cell adhesion and communication. Relocation of SMAGP from the membrane to the cytosol is associated with tissue disorganization and metastatic transformation. Over the extracellular and cytoplasmic domains, rat SMAGP shares 96% and 66% aa sequence identity with mouse and human SMAGP, respectively.

Long Name

Small Transmembrane and Glycosylated Protein

Entrez Gene IDs

57228 (Human); 207818 (Mouse); 300236 (Rat)

Alternate Names

hSMAGP; LOC57228; MGC149453; MGC149454; SMAGP; small cell adhesion glycoprotein; small cell transmembrane and glycosylated protein; Small transmembrane and glycosylated protein; small trans-membrane and glycosylated protein