Rat Synaptotagmin-1 Antibody

Catalog #: MAB43641
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB43641
MAB43641-SP

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Synaptotagmin-1 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Synaptotagmin‑1 in Western blots. Detects Synaptotagmin-1 from rat, mouse, human, chicken, Xenopus, and fish.

Source

Monoclonal Mouse IgG_2A Clone # ASV30

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Rat brain synaptic plasma membranes

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

Matthew, W.D. et al. (1981) J. Cell Biol. 91:257. This application was not tested by R&D Systems.

Immunohistochemistry

8-25 µg/mL

See below

Immunoprecipitation

Matthew, W.D. et al. (1981) J. Cell Biol. 91:257.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Synaptotagmin-1 in Rat Spinal Cord. Synaptotagmin-1 was detected in perfusion fixed frozen sections of rat spinal cord using 8 µg/mL Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB43641) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Synaptotagmin-1

The Synaptotagmins are a family of integral membrane proteins of synaptic vesicles. Rat Synaptotagmin-1 (Accession # P21707) is a 57 kDa, 421 aa glycoprotein containing two C2 domains related to protein kinase C, and sites for palmitoylation and binding of acidic phospholipids, calcium and calmodulin. Synaptotagmin-1 participates in the process of vesicular trafficking and exocytosis by inducing local Ca2⁺-dependent buckling of the plasma membrane. Rat Synaptotagmin-1 shares 98% aa identity or greater with mouse, human and bovine Synaptotagmin-1.

Entrez Gene IDs

6857 (Human); 20979 (Mouse); 25716 (Rat)

Alternate Names

DKFZp781D2042; P65; SVP65; synaptotagmin Isynaptotagmin 1; Synaptotagmin1; Synaptotagmin-1; SYT; SYT1; sytI

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Rat Synaptotagmin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1