Rat TIMP-1 Antibody

Catalog #: MAB5802 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5802
MAB5802-SP

All TIMP-1 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat TIMP-1 Antibody Summary

Species Reactivity

Rat

Specificity

Detects recombinant rat TIMP-1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 145701

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat TIMP-1
Cys24-Ala217
Accession # P30120

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Rat TIMP-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Rat TIMP‑1 Biotinylated Antibody (Catalog # BAF580)

Standard: Recombinant Rat TIMP-1 Protein, CF (Catalog # 580-RT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-1

Tissue Inhibitor of Metalloproteinases-1, (TIMP-1) belongs to a family of proteins that regulate the activation and proteolytic activity of the matrix metalloproteinases (MMPs). TIMP-1 is a glycoprotein produced by a wide range of cell types. It inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex that may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to play a role in tissue homeostasis.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Alternate Names

CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1