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Recombinant Cyno Monkey LILRB4/CD85k/ILT3 Fc Protein, CF

Catalog #: 11420-T4 Datasheet / COA / SDS
Fc Chimera
Catalog # Availability Size / Price Qty
11420-T4-050
Recombinant Cynomolgus Monkey LILRB4/CD85k/ILT3 Fc Chimera Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cyno Monkey LILRB4/CD85k/ILT3 Fc Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Cynomolgus Monkey LILRB4/CD85k/ILT3 Fc Chimera binds to Recombinant Human Apolipoprotein E3 Protein (Catalog # 4144‑AE) with a ED50 of 0.900-9.00 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey LILRB4/CD85k/ILT3 protein
Cynomolgus LILRB-4
(Gly24-Glu259)
Accession # XP_015297198.1		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
XP_015297198.1
N-terminal Sequence
Analysis
Gly24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
53 kDa
SDS-PAGE
55-66 kDa, under reducing conditions.

Product Datasheets

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11420-T4

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11420-T4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

Measured by its binding ability in a functional ELISA. Recombinant Cynomolgus Monkey LILRB4/CD85k/ILT3 Fc Chimera (Catalog # 11420-T4) binds to Recombinant Human Apolipoprotein E3 Protein (4144-AE) with a ED50 of 0.900-9.00 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey LILRB4/CD85k/ILT3 Fc Chimera Protein (Catalog # 11420-T4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-66 kDa and 110-130 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRB4/CD85k/ILT3

LILRB4, also known as ILT3, CD85k, and LIR5, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation (1). Based on the similarity as human LILRB4, mature cynomolgus monkey LILRB4 is predicted to consist of an extracellular domain (ECD) with two Ig-like domains, a transmembrane segment, and a cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). The mature ECD of cynomolgus monkey LILRB4 shares 81% and 99% amino acid identity with human and rhesus LILRB4, respectively. Alternative splicing of LILRB4 generates an isoform that lacks the first ITIM and a secreted isoform that circulates in the serum of cancer patients (3, 4). LILRB4 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC) (2, 5, 6). Ligation of LILRB4 triggers ITIM-mediated inhibition of cell-activating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection (2, 4, 7, 8). Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL (4, 7, 9). In turn, CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC (5, 6, 8, 10, 11). Recently, a novel anti-LILRB4 CAR-T Cell was been used to treat monocytic acute myeloid leukemia in humanized hematopoietic-reconstituted mice models (12).

References
  1. Vlad, G. et al. (2010) Int. Rev. Immunol. 29:119.
  2. Cella, M. et al. (1997) J. Exp. Med. 185:1743.
  3. Heinzmann, A. et al. (2000) Eur. J. Immunogenet. 27:121.
  4. Suciu-Foca, N. et al. (2007) J. Immunol. 178:7432.
  5. Gleissner, C.A. et al. (2007) Eur. J. Immunol. 37:177.
  6. Manavalan, J.S. et al. (2004) Int. Immunol. 16:1055.
  7. Vlad, G. and N. Suciu-Foca (2012) Exp. Mol. Pathol. 93:294.
  8. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
  9. Vlad, G. et al. (2006) Int. Immunopharmacol. 6:1889.
  10. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
  11. Brenk, M. et al. (2009) J. Immunol. 183:145.
  12. John, S. et al. (2018) Mol. Ther. 26:2487.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
Entrez Gene IDs
11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)
Alternate Names
CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4

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