Recombinant Cynomolgus CD200R1 Fc Chimera Protein, CF Summary
Product Specifications
| Human Cynomolgus Monkey CD200 R1 (Ala27-Leu267) Accession # XP_005548208 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9357-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 1 mg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Cynomolgus CD200 R1 Fc Chimera (Catalog # 9357-CD) is immobilized at 2 µg/mL, 100 µL/well, Recombinant Human CD200 Fc Chimera Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) binds with an ED50 of 1.5-9 ng/mL.
Reconstitution Calculator
Background: CD200R1
CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1-3). The cynomolgus CD200 R1 cDNA encodes a 241 aa extracellular domain (ECD) and 61 aa cytoplasmic tail. The ECD is composed of one Ig-like V‑type domain and one Ig-like C2-type domain (4). Within the ECD, cynomolgus CD200 R1 shares 91%, 54%, and 57% aa sequence identity with human, mouse, and rat CD200 R1, respectively. At least two alternate splice isoforms exist that differ in their cytoplasmic domains. CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells (5-7), while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). CD200 R1 propagates inhibitory signals despite its lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif) (6, 7, 11, 12).
- Rosenblum, M.D. et al. 2006, J. Dermatol. Sci. 41:165.
- Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
- Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
- Wright, G.J. et al. (2003) J. Immunol. 171:3034.
- Shiratori, I. et al. (2005) J. Immunol. 175:4441.
- Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
- Fallarino, F. et al. (2004) J. Immunol. 173:3748.
- Wright, G.J. et al. (2001) Immunology 102:173.
- Hoek, R.M. et al. (2000) Science 290:1768.
- Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
- Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
- Zhang, S. et al. (2004) J. Immunol. 173:6786.
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