Recombinant Cynomolgus Mesothelin (aa417-693) Fc Protein, CF
Recombinant Cynomolgus Mesothelin (aa417-693) Fc Protein, CF Summary
Product Specifications
| Cynomolgus Monkey Mesothelin (Asp417-Asp693) Accession # XP_015298164.1 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10760-MS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 1 mg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human CA125/MUC16 (5609-MU) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Cynomolgus Mesothelin (aa 417-693) Fc Chimera (Catalog # 10760-MS) binds with an ED50 of 4.0-20.0 ng/mL.
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2 μg/lane of Recombinant Cynomolgus Monkey Mesothelin (aa 417-693) Fc Chimera (Catalog # 10760-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-85 kDa and 130-170 kDa, respectively.
Reconstitution Calculator
Background: Mesothelin
Mesothelin (MSLN), also known as CAK1 and ERC, is a glycosylated cell-surface antigen present on normal mesothelial cells and overexpressed in several human tumors (1, 2). The mesothelin gene encodes a ~70 kDa precursor protein that is cleaved at a dibasic proteolytic site into a 40-kDa membrane-bound protein termed MSLN and a 31-kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell (3, 4). Cleaved cynomolgus MSLN remains attached to the cell surface via a GPI linkage and shares 84% amino acid sequence identity with human MSLN. In human, alternate splicing generates additional MSLN isoforms that have either an eight amino acid insertion following Ser408 or a substituted C‑terminal region with no GPI anchor (3). Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 4). It is upregulated in mesotheliomas and a range of carcinomas and adenomas (5-7). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression (8, 9). It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (10). A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions (10-12).
- Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
- Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
- Muminova, Z.E. et al. (2004) BMC Cancer 4:19.
- Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
- Ordonez, N.G. (2003) Mod. Pathol. 16:192.
- Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
- Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
- Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
- Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
- Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
- Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
- Robinson, B.W.S. et al. (2003) Lancet 362:1612.
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