Recombinant Guinea Pig IL-1 beta/IL-1F2 Protein Summary
Product Specifications
The ED50 for this effect is 7‑35 pg/mL.
Thr115-Ser266, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6226-GL
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES, NaCl, DTT, and EDTA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
6226-GL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES, NaCl, DTT, and EDTA. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 18% amino acid (aa) identity in guinea pig. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 R1 binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL‑1 R3/IL‑1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 R2 has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL‑1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL‑1 R1 (1 ‑ 4). The guinea pig IL-1 beta cDNA encodes a 266 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature guinea pig IL-1 beta shares 64% ‑ 80% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus IL‑1 beta.
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- Accession # Q9WVG1.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
FAQs
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Does IL-1 beta induce NO in smooth muscle?
Yes it does, based on the literature.
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