Recombinant Human Attractin Protein, CF Summary
Product Specifications
| Human Attractin (Ala84-Gln1272) Accession # NP_647538 |
HPGGGSGGGSGGGS |
6-His tag |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7238-AT
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Attractin
Attractin, also known as DPPT-L and Mahogany, is a transmembrane glycoprotein with functions in hair pigmentation, axon myelination, energy balance regulation, and immune activation (1, 2). Mature human Attractin consists of a 1196 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 129 aa cytoplasmic domain (3). Within the ECD, it shares 95% aa sequence identity with mouse and rat Attractin. Alternative splicing generates an approximately 175 kDa secreted soluble isoform that corresponds to the ECD of the transmembrane form (4, 5). Attractin is expressed in activated T cells, monocytes, hair follicle melanocytes, brain (notably the hypothalamus, hippocampus, and substantia nigra), and adipose tissue (3, 6-9). Activated T cells release soluble Attractin which then co-stimulates T cell proliferation (3, 4). Attractin also functions with Melanocortin 1R as a co-receptor for Agouti and, as a soluble molecule, can neutralize the bioactivity of Agouti on hair follicle melanocytes (7, 8, 10, 11). Similarly, mutations in Attractin gene suppresses the diet-induced obesity which is characteristic of Agouti overexpressing mice (1, 7). In the CNS, Attractin plays a role in axon myelination, neurite branching on differentiating neurons, and protection from neurotoxins (1, 9, 12). It is elevated in the CSF in high grade malignant astrocytoma and promotes glioma cell migration (14).
- Gunn, T.M. et al. (2001) Genetics 158:1683.
- Friedrich, D. et al. (2007) Biol. Chem. 388:155.
- Duke-Cohan, J.S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:11336.
- Duke-Cohan, J.S. et al. (1996) J. Immunol. 156:1714.
- Tang, W. et al. (2000) Proc. Natl. Acad. Sci. USA 97:6025.
- Wrenger, S. et al. (2006) J. Leukoc. Biol. 80:621.
- Nagle, D.L. et al. (1999) Nature 398:148.
- He, L. et al. (2001) Nat. Genet. 27:40.
- Paz, J. et al. (2007) Neurobiol. Aging 28:1446.
- Hida, T. et al. (2009) Pigment Cell Melanoma Res. 22:623.
- Gunn, T.M. et al. (1999) Nature 398:152.
- Kuramoto, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:559.
- Tang, W. and J.S. Duke-Cohan (2002) J. Neuropathol. Exp. Neurol. 61:767.
- Khwaja, F.W. et al. (2006) Clin. Cancer Res. 12:6331.
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