Recombinant Human CD24 Fc Chimera Avi-tag Protein, CF
Recombinant Human CD24 Fc Chimera Avi-tag Protein, CF Summary
Learn more about Avi-tag Biotinylated ProteinsProduct Specifications
| Human CD24 (Ser27-Gly59) Accession # P25063.3 | IEGRMD | Human IgG1 (Pro100-Lys330) | Avi-tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
AVI5247
| Formulation | Supplied as a 0.2 μm filtered solution in PBS. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human Siglec-10 Fc Chimera (2130-SL) is coated at 5 μg/mL, the concentration of Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag (Catalog # AVI5247) that produces 50% optimal binding response is found to be approximately 1.5-7.5 μg/mL.
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2 μg/lane of Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag Protein (Catalog # AVI5247) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-55 kDa and 80-100 kDa, respectively.
Reconstitution Calculator
Background: CD24
CD24, also known as Heat-Stable Antigen and Nectadrin, is a heavily and variably glycosylated 30 kDa-60 kDa GPI-linked sialoprotein (1-4). Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells (4-6). In mouse, CD24 is even more widely expressed, particularly on T cells, monocytes, and dendritic cells (2). CD24 expression is regulated during lineage development and with the activation of various cell types (4). Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance (7-11). CD24 on antigen presenting cells cooperates with B7 molecules in the co-stimulation of T cells (12-14). CD24 associates in cis with Siglec-10 (or Siglec-G in mouse) and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells (15). Formation of these ternary complexes fills a protective role: the resulting Siglec-10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs (15, 16). Mature human CD24 shares 30% and 42% amino acid sequence identity with mouse and rat CD24, respectively. Our Avi-tag Biotinylated human CD24 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
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