Recombinant Human CD43 Protein, CF Summary
Product Specifications
Ser20-Arg253, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9680-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human CD43 binds to Recombinant Human Siglec-1/CD169 (Catalog # 5197-SL) binds with an ED50 of 1.00-10.0 µg/mL.
Reconstitution Calculator
Background: CD43
CD43, also known as Leukosialin and Sialophorin and Ly-48, is a type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells (1). Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific (2). CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion (3). In human, CD43 is synthesized as a 400 amino acid (aa) precursor that contains a 19 aa signal sequence, a 234 aa extracellular region, a 23 aa TM domain, and a 124 aa cytoplasmic tail. The extracellular region (aa 20-253) has 25 O-linked glycosylation sites and 1 N-linked glycosylation site. Human CD43 extracellular region has less than 60% aa identity to the extracellular regions in rat and mouse.
- Santamaría, M. et al. (1996). Cancer Res. 56:3526.
- Manjunath, N. et al. (1995) Nature 377:535.
- Brown, T. J. et al. (1996) J. Biol. Chem. 271:27686.
Citations for Recombinant Human CD43 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bond
Authors: BJ Medley, L Leclaire, N Thompson, KE Mahoney, B Pluvinage, MAH Parson, JE Burke, S Malaker, W Wakarchuk, AB Boraston
The Journal of Biological Chemistry, 2022-08-30;0(0):102439.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences
Authors: DJ Shon, D Fernandez, NM Riley, MJ Ferracane, CR Bertozzi
The Journal of Biological Chemistry, 2022-04-09;0(0):101917.
Species: Akkermansia muciniphila
Sample Types: Protein
Applications: Bioassay
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