Recombinant Human CD43 Protein, CF

Catalog # Availability Size / Price Qty
9680-CD-050
Recombinant Human CD43 Protein Binding Activity
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Product Details
Citations (2)
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Recombinant Human CD43 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Human CD43 binds to Recombinant Human Siglec‑1/CD169 (Catalog # 5197-SL) with an ED50 of 1.00-10.0  μg/mL.
Source
Mouse myeloma cell line, NS0-derived human CD43 protein
Ser20-Arg253, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Ser20
Predicted Molecular Mass
25 kDa
SDS-PAGE
77-88 kDa, reducing conditions

Product Datasheets

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9680-CD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9680-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human CD43 Protein Binding Activity View Larger

Recombinant Human CD43 binds to Recombinant Human Siglec-1/CD169 (Catalog # 5197-SL) binds with an ED50 of 1.00-10.0 µg/mL.

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Background: CD43

CD43, also known as Leukosialin and Sialophorin and Ly-48, is a type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells (1). Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific (2). CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion (3). In human, CD43 is synthesized as a 400 amino acid (aa) precursor that contains a 19 aa signal sequence, a 234 aa extracellular region, a 23 aa TM domain, and a 124 aa cytoplasmic tail. The extracellular region (aa 20-253) has 25 O-linked glycosylation sites and 1 N-linked glycosylation site. Human CD43 extracellular region has less than 60% aa identity to the extracellular regions in rat and mouse.

References
  1. Santamaría, M. et al. (1996). Cancer Res. 56:3526.
  2. Manjunath, N. et al. (1995) Nature 377:535.
  3. Brown, T. J. et al. (1996) J. Biol. Chem. 271:27686.
Long Name
Cluster of Differentiation 43
Entrez Gene IDs
6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)
Alternate Names
CD43 antigen; CD43; CD43); Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115, leukosialin, CD43); Sialophorin; SPN

Citations for Recombinant Human CD43 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bond
    Authors: BJ Medley, L Leclaire, N Thompson, KE Mahoney, B Pluvinage, MAH Parson, JE Burke, S Malaker, W Wakarchuk, AB Boraston
    The Journal of Biological Chemistry, 2022-08-30;0(0):102439.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences
    Authors: DJ Shon, D Fernandez, NM Riley, MJ Ferracane, CR Bertozzi
    The Journal of Biological Chemistry, 2022-04-09;0(0):101917.
    Species: Akkermansia muciniphila
    Sample Types: Protein
    Applications: Bioassay

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