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Recombinant Human CD63 Fc Chimera Protein, CF

Catalog #: 5048-CD Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5048-CD-050
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Product Details
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD63 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human BST-2/Tetherin (Catalog # 9939-BS) is immobilized at 2.5 μg/mL (100 μL/well), Recombinant CD63 Fc Chimera binds with an ED50 of 1-6 μg/mL.   
Source
Chinese Hamster Ovary cell line, CHO-derived human CD63 protein
MD
Human IgG1
(Pro100-Lys330)		IEGR Human CD63
(Ala103-Val203)
Accession # P08962-1
N-terminusC-terminus
Accession #
P08962-1
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
38 kDa
SDS-PAGE
41-60 kDa, reducing conditions

Product Datasheets

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5048-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5048-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human BST-2/Tethrin (9939-BS) is immodibilized at 2.5 μg/mL, 100 μL/well, Recombinant Human CD63 Fc Chimera (Catalog # 5048-CD) binds with an ED50 of 1-6 μg/mL.

SDS-PAGE Recombinant Human CD63 Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human CD63 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-60 kDa and 80-120 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD63

CD63, also known as Tspan-30, is a member of the tetraspanin family of proteins that mediate signal transduction events which play a role in the regulation of cell development, activation, growth and motility (1, 2). These membrane proteins are characterized by the presence of four transmembrane domains, a small extracellular loop (SEL) and a large extracellular loop (LEL) (2, 3). Regions of the tetraspanin LEL are known to associate with integrins to regulate membrane protein trafficking, leukocyte recruitment, and adhesion processes (4, 5). Deficiency of CD63 is associated with Hermansky-Pudlak syndrome (6). Human CD63-LEL shares 67% and 66% aa sequence identity with mouse and rat CD63-LEL, respectively.  CD63 has been identified as a cell surface binding partner for tissue inhibitor of metalloproteinase 1 (TIMP-1), modulating the tetraspanin/integrin signaling complex which regulates cell survival and polarization (7).

References
  1. Kang, M. et al. (2014) PLOS One. 9:e102817.
  2. Yu, M. et al. (2016) J. Immunol. Res. 2016:1.
  3. Chaiyadet, S. et al. (2017) Sci. Rep 7:14342.
  4. Tugues, S. et al. (2013) J. Biol. Chem. 288:19060.
  5. Yañez-Mo, M. et al. (2009) Trends Cell Biol. 19:434.
  6. Nishibori, M. et al. (1993) J. Clin. Invest. 91:1775.
  7. Jung, K.K. et al. (2006) EMBO J. 25:3934.
Entrez Gene IDs
967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)
Alternate Names
CD_antigen: CD63; CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30

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