Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein, CF

Catalog #: AVI10927 Datasheet / COA / SDS

Biotinylated

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AVI10927-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Human CD8 alpha Antibody (Catalog # MAB3803) is immobilized at 2 μg/mL, 100 μg/mL, Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag binds with an ED₅₀ of 2.00-12.0 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CD8 protein

Human CD8 alpha (Ser22-Asp182) Accession # P01732.1	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P01732.1

N-terminal Sequence
Analysis

Ser22

Structure / Form

Biotinylated via Avi-tag.

Predicted Molecular Mass

46 kDa

SDS-PAGE

55-61 kDa, under reducing conditions.

Product Datasheets

AVI10927

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10927

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Human CD8 alpha Antibody (Catalog # MAB3803) is immobilized at 2 μg/mL, 100 μg/mL, Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag (AVI10927) binds with an ED₅₀ of 2.00-12.0 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein (Catalog # AVI10927) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-61 kDa and 110-120 kDa, respectively.

Reconstitution Calculator

Background: CD8

CD8, also known as Ly2 or Leu2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes (1, 2). CD8 alpha is expressed on double positive (CD4+ CD8+) thymocytes and mature CD8+ cytolytic T cells (CTL) (3-5), intraepithelial lymphocytes (IEL) (6), some gamma δ T cells (7), subsets of thymic and splenic dendritic cells (DC) (8), and peritoneal mast cells (9). It can form disulfide linked homodimers or heterodimers with CD8 beta (10). Thymic CD8+ DC express primarily alpha beta heterodimers, while splenic CD8+ DC primarily express alpha alpha homodimers (8). CD8 alpha + DC can present viral antigenic peptides in complex with MHC I and prime CTL responses (11). The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Iglike domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (12). Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively. Our Avi-tag Biotinylated human CD8 alpha features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of bionylation and the rest of the protein is unchanged so there is no interference in the protein bioactivity.

References

Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
Cole, D.K. et al. (2012) Immunology 137:139.
Germain, R.N. (2002) Nat. Rev. Immunol. 2:309.
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
Nakayama, K. et al. (1994) Science 263:1131.
Wang, J. and J.R. Klein (1994) Science 265:1860.
MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
Vremec, D. et al. (1992) J. Exp. Med. 176:47.
Lin, T.J. et al. (1998) J. Immunol. 161:6265.
Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
Belz, G.T. et al. (2004) J. Immunol. 172:1996.
Nakauchi, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5126.

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Alternate Names

CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2