Recombinant Human CRACC/SLAMF7 His-tag Avi-tag Protein, CF
Recombinant Human CRACC/SLAMF7 His-tag Avi-tag Protein, CF Summary
Product Specifications
Ser23-Met226, With a C-terminal 6-His tag & Avi-tag
Analysis
Biotinylated via Avi-tag
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
AVI11533
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Biotinylated Recombinant Human CRACC/SLAMF7 His-tag Protein (Catalog # AVI11533) binds Human CRACC/SLAMF7 Antibody (MAB19061) with an ED50 of 5.00-60.0 ng/mL.
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2 μg/lane of Biotinylated Recombinant Human CRACC/SLAMF7 His-tag Protein (Catalog # AVI11533) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-52 kDa, under reducing conditions.
Reconstitution Calculator
Background: CRACC/SLAMF7
CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature human CRACC consists of an extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain, a transmembrane segment, and a cytoplasmic domain with one immunoreceptor tyrosine-based switch motif (ITSM) (2, 3). Within the ECD, human CRACC shares 54% and 52% amino acid sequence identity with mouse and rat CRACC, respectively. There are seven known isoforms of CRACC which are distinguished by deletions and/or substitutions in both their ECD and cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8+ T cells, activated B cells, and mature dendritic cells (4, 5). Its homophilic interaction induces NK, CTL, and B cell activation (4-7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8).Our Avi-tag Biotinylated Recombinant Human CRACC/SLAMF7 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
- Veillette, A. (2006) Immunol. Rev. 214:22.
- Tovar, V. et al. (2002) Immunogenetics 54:394.
- Murphy, J.J. et al. (2002) Biochem. J. 361:431.
- Bouchon, A. et al. (2001) J. Immunol. 167:5517.
- Lee, J.K. et al. (2007) J. Immunol. 179:4672.
- Kumaresan, P.R. et al. (2002) Mol. Immunol. 39:1.
- Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
- Tassi, H. and M. Colonna (2005) J. Immunol. 175:7996.
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