Recombinant Human EGFR Fc Chimera Alexa Fluor® 647 Protein

Catalog #: AFR344 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AFR344-020

Flow cytometry analysis for Recombinant Human EGFR Fc-tag Alexa Fluor® 647 staining on Anti-Human EGFR Monoclonal Antibody conjugated beads.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EGFR Fc Chimera Alexa Fluor® 647 Protein Summary

Learn more about Fluorokines^TM Fluorescent-Labeled Proteins

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by flow cytometry for its ability to bind anti-human EGFR Monoclonal Antibody conjugated beads. The concentration of Recombinant Human EGFR Fc-tag Alexa Fluor® 647 (Catalog # AFR344) that produces 50% of the binding response is 0.750-15.0 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human EGFR protein

Human EGFR (Leu25-Ser645) Accession # CAA25240.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

CAA25240.1

N-terminal Sequence
Analysis

Leu25

Structure / Form

Disulfide-linked homodimer

Labeled with Alexa Fluor® 647

Excitation Wavelength: 650 nm

Emission Wavelength: 668 nm

Predicted Molecular Mass

95 kDa (monomer)

SDS-PAGE

118-134 kDa, under reducing conditions.

Product Datasheets

AFR344

Product Datasheet

COA

SDS

AFR344

Formulation	Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Flow Cytometry

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Streptavidin coated beads conjugated to biotinylated Anti-Human EGFR were stained with the indicated concentrations of Recombinant Human EGFR Fc-tag Alexa Fluor® 647 (Catalog # AFR344).

SDS-PAGE

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2 μg/lane of Recombinant Human EGFR Fc Chimera Alexa Fluor® 647 Protein (Catalog # AFR344) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 118-134 kDa and 236-268 kDa, respectively.

Reconstitution Calculator

Background: EGFR

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular ligand binding domain containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The human EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 621 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 542 aa cytoplasmic domain (3, 4). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (5‑7). Within the ECD, human EGFR shares 88% aa sequence identity with mouse and rat EGFR. It shares 43%-44% aa sequence identity with the ECD of human ErbB2, ErbB3, and ErbB4. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (8‑12). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (13, 14). EGFR is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs (15).

References

Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
Shilo, B.Z. (2005) Development 132:4017.
Lin, C. et al. (1984) Science 224:843.
Ullrich, A. et al. (1984) Nature 309:418.
Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050.
Reiter J.L. et al. (2001) Genomics 71:1.
Xu, Y.H. et al. (1984) Nature 309:806.
Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
Yarden, Y. et al. (1987) Biochemistry 26:1434.
Burgess, A.W. et al. (2003) Mol. Cell 12:541.
Lemmon, M.A. et al. (1997) EMBO J. 16:281.
Cohen, S. et al. (1982) J. Biol. Chem. 257:1523.
Sibilia, M. and E.F. Wagner (1995) Science 269:234.
Miettinen, P.J. et al. (1995) Nature 376:337.
Roskoski Jr., R. (2004) Biochem. Biophys. Res. Commun. 319:1.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.