Home / Fc gamma RIIIA/CD16a / Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

Catalog #: 4325-FC
21 Citations 6 Reviews Datasheet / COA / SDS
Analyzed by SEC-MALS, Isoform V176, His-tag
Catalog # Availability Size / Price Qty
4325-FC-050
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Recombinant Human Fc gamma RIIIA/CD16a Protein SEC-MALS.
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Product Details
Citations (21)
FAQs
Supplemental Products
Reviews (6)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fc gamma RIIIA/CD16a Protein, CF Summary

Why choose R&D Systems Fc gamma RIIIA Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 90%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind human IgG with an estimated Kd <50 nM.
Source
Mouse myeloma cell line, NS0-derived human Fc gamma RIIIA/CD16a protein
Gly17-Gln208, with a C-terminal 6-His tag
Accession #
AAH17865
N-terminal Sequence
Analysis
Gly17
Predicted Molecular Mass
22.6 kDa
SDS-PAGE
40-50 kDa, reducing conditions

Product Datasheets

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4325-FC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4325-FC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Human Fc gamma RIIIA/CD16a Protein (Catalog # 4325-FC) has a molecular weight (MW) of 33.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Binding Activity Recombinant Human Fc gamma RIIIA/CD16a Protein Binding Activity View Larger

Recombinant Human Fc gamma RIIIA/CD16a (Catalog # 4325-FC) binds human IgG with an estimated Kd<50 nM.

SDS-PAGE Recombinant Human Fc gamma RIIIA/CD16a Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human Fc gamma RIIIA/CD16a was resolved with SDS‑PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 40-50 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIIA/CD16a

Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes (1, 2). In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages (1). Fc gamma RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils (1, 2). The ECD of Fc gamma RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIa, feline CD16, bovine CD16 and porcine Fc gamma RIIIb paralogs, respectively. The Fc gamma RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 191 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 22 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism creates high binding (176V) and low binding (176F) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (3, 4). Catalog # 4325-FC is expressed as the 176V isoform of Fc gamma RIIIa. Fc gamma RIIIa surface expression requires interaction of an accessory chain, either the common gamma -chain or CD3 zeta (5, 6). Glycosylation patterns, electrophoretic mobility and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIa (7). The ECD of both Fc gamma RIIIa and b can be proteolytically cleaved and retain binding activity in soluble form (8-11). In monocytes and macrophages, activation and phagocytosis can trigger Fc gamma RIIIa release (11). Soluble Fc gamma RIII can be detected in normal plasma and is increased in rheumatoid arthritis and in coronary artery diseases (9, 10).

References
  1. Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19. 
  2. Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481. 
  3. Wu, J. et al. (1997) J. Clin. Invest. 100:1059. 
  4. Dall’Ozzo, S. et al. (2004) Cancer Res. 64:4664. 
  5. Kim, M.-K. et al. (2003) Blood 101:4479. 
  6. Lanier, L.L. et al. (1989) Nature 342:803.
  7. Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
  8. Li, P. et al. (2007) J. Biol. Chem. 282:6210.
  9. Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
  10. Masuda, M. et al. (2006) Atherosclerosis 188:377.
  11. Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.
Long Name
Fc gamma Receptor III A
Entrez Gene IDs
2214 (Human); 304966 (Rat)
Alternate Names
CD16a antigen; CD16a; CD16FCRIIIA; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc gamma receptor III-A; Fc gamma RIIIA; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; FCRIII; FcRIIIa; IGFR3FcgammaRIIIA; immunoglobulin G Fc receptor III; low affinity III, receptor for (CD16); low affinity immunoglobulin gamma Fc region receptor III-A; neutrophil-specific antigen NA

Citations for Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma
    Authors: Pascual-Pasto, G;McIntyre, B;Hines, MG;Giudice, AM;Garcia-Gerique, L;Hoffmann, J;Mishra, P;Matlaga, S;Lombardi, S;Shraim, R;Schürch, PM;Yarmarkovich, M;Hofmann, TJ;Alikarami, F;Martinez, D;Tsang, M;Gil-de-Gómez, L;Spear, TT;Bernt, KM;Wolpaw, AJ;Dimitrov, DS;Li, W;Bosse, KR;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A Dual-Approach Strategy to Optimize the Safety and Efficacy of Anti-Zika Virus Monoclonal Antibody Therapeutics
    Authors: Sun, H;Yang, M;Lai, H;Neupane, B;Teh, AY;Jugler, C;Ma, JK;Steinkellner, H;Bai, F;Chen, Q;
    Viruses
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR)
  3. Optimized ACE2 decoys neutralize antibody-resistant SARS-CoV-2 variants through functional receptor mimicry and treat infection in vivo
    Authors: JA Torchia, AH Tavares, LS Carstensen, DY Chen, J Huang, T Xiao, S Mukherjee, PM Reeves, H Tu, AE Sluder, B Chen, DN Kotton, RA Bowen, M Saeed, MC Poznansky, GJ Freeman
    Science Advances, 2022-12-07;8(49):eabq6527.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  4. BNT162b2-induced neutralizing and non-neutralizing antibody functions against SARS-CoV-2 diminish with age
    Authors: TA Bates, P Lu, YJ Kang, D Schoen, M Thornton, SK McBride, C Park, D Kim, WB Messer, ME Curlin, FG Tafesse, LL Lu
    Cell Reports, 2022-10-05;41(4):111544.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. Discovery of a novel Igbeta and FcgammaRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus
    Authors: Y Kitanaga, D Yamajuku, S Kubo, K Nakamura, M Maeda, M Seki, Y Kaneko, F Kinugasa, T Morokata, Y Kondo, H Yoshinari, S Nakayamada, T Sumida, Y Tanaka
    International immunopharmacology, 2021-11-12;101(0):108343.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  6. High neutralizing potency of swine glyco-humanized polyclonal antibodies against SARS-CoV-2
    Authors: B Vanhove, O Duvaux, J Rousse, PJ Royer, G Evanno, C Ciron, E Lheriteau, L Vacher, N Gervois, R Oger, Y Jacques, S Conchon, A Salama, R Duchi, I Lagutina, A Perota, P Delahaut, M Ledure, M Paulus, RT So, CK Mok, R Bruzzone, M Bouillet, S Brouard, E Cozzi, C Galli, D Blanchard, JM Bach, JP Soulillou
    European Journal of Immunology, 2021-03-02;0(0):.
    Species: Porcine
    Sample Types: Protein
    Applications: Bioassay
  7. Monoclonal antibody formulation manufactured by high-speed electrospinning
    Authors: J Domján, P Vass, E Hirsch, E Szabó, E Pantea, SK Andersen, T Vigh, G Verreck, G Marosi, ZK Nagy
    Int J Pharm, 2020-11-04;0(0):120042.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  8. Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus
    Authors: PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass
    J. Immunol., 2019-01-25;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture), Surface Plasmon Resonance
  9. An antibody with Fab-constant domains exchanged for a pair of CH3 domains
    Authors: G Wozniak-Kn, G Stadlmayr, JW Perthold, K Stadlbauer, M Gotsmy, S Becker, F Rüker
    PLoS ONE, 2018-04-09;13(4):e0195442.
    Applications: Bioassay
  10. Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies
    Authors: F Arce Varga, AJS Furness, K Litchfield, K Joshi, R Rosenthal, E Ghorani, I Solomon, MH Lesko, N Ruef, C Roddie, JY Henry, L Spain, A Ben Aissa, A Georgiou, YNS Wong, M Smith, D Strauss, A Hayes, D Nicol, T O'Brien, L Mårtensson, A Ljungars, I Teige, B Frendéus, M Pule, T Marafioti, M Gore, J Larkin, S Turajlic, C Swanton, KS Peggs, SA Quezada
    Cancer Cell, 2018-03-22;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  11. Glycosylation profile and biological activity of Remicade� compared with Flixabi� and Remsima�
    Authors: C Lee, M Jeong, JJ Lee, S Seo, SC Cho, W Zhang, O Jaquez
    MAbs, 2017-06-22;0(0):0.
    Applications: Binding Assay
  12. Neuregulin 1 allosterically enhances the anti-tumor effects of the non-competing anti-HER3 antibody 9F7-F11 by increasing its binding to HER3
    Authors: C Le Clorenn, H Bazin, O Dubreuil, C Larbouret, C Ogier, Y Lazrek, V Garambois, MA Poul, P Mondon, JM Barret, G Mathis, JF Prost, A Pèlegrin, T Chardès
    Mol. Cancer Ther., 2017-05-15;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  13. An Anti-EGFR IgA That Displays Improved Pharmacokinetics and Myeloid Effector Cell Engagement In Vivo.
    Authors: Lohse S, Meyer S, Meulenbroek L, Jansen J, Nederend M, Kretschmer A, Klausz K, Moginger U, Derer S, Rosner T, Kellner C, Schewe D, Sondermann P, Tiwari S, Kolarich D, Peipp M, Leusen J, Valerius T
    Cancer Res, 2015-12-03;76(2):403-17.
    Applications: Surface Plasmon Resonance
  14. Mechanisms of protection against Clostridium difficile infection by the monoclonal antitoxin antibodies actoxumab and bezlotoxumab.
    Authors: Yang Z, Ramsey J, Hamza T, Zhang Y, Li S, Yfantis H, Lee D, Hernandez L, Seghezzi W, Furneisen J, Davis N, Therien A, Feng H
    Infect Immun, 2014-12-08;83(2):822-31.
    Species: Human
    Sample Types: Antibody
    Applications: Bioassay
  15. Integrin receptors on tumor cells facilitate NK cell-mediated antibody-dependent cytotoxicity.
    Authors: Anikeeva N, Steblyanko M, Fayngerts S, Kopylova N, Marshall D, Powers G, Sato T, Campbell K, Sykulev Y
    Eur J Immunol, 2014-06-05;44(8):2331-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases.
    Authors: Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A
    J Immunol, 2014-03-26;192(9):4083-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  17. ADAM17-mediated shedding of FcgammaRIIIA on human NK cells: identification of the cleavage site and relationship with activation.
    Authors: Lajoie L, Congy-Jolivet N, Bolzec A, Gouilleux-Gruart V, Sicard E, Sung H, Peiretti F, Moreau T, Vie H, Clemenceau B, Thibault G
    J Immunol, 2013-12-13;192(2):741-51.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  18. Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors.
    Authors: Franco A, Damdinsuren B, Ise T, Dement-Brown J, Li H, Nagata S, Tolnay M
    J Immunol, 2013-04-24;190(11):5739-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  19. Bi-specific Aptamers Mediating Tumor Cell Lysis.
    Authors: Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B
    J. Biol. Chem., 2011-04-29;286(24):21896-905.
    Species: Human
    Sample Types: DNA
    Applications: EMSA
  20. Production of non-fucosylated antibodies by co-expression of heterologous GDP-6-deoxy-D-lyxo-4-hexulose reductase.
    Authors: von Horsten HH, Ogorek C, Blanchard V, Demmler C, Giese C, Winkler K, Kaup M, Berger M, Jordan I, Sandig V
    Glycobiology, 2010-07-15;20(12):1607-18.
    Species: Human
    Sample Types: Antibody
    Applications: Bioassay
  21. Glycoengineering of human IgG1-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation.
    Authors: Wei Y, Li C, Huang W, Li B, Strome S, Wang LX
    Biochemistry, 2008-09-05;47(39):10294-304.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

FAQs

  1. What is the amino acid sequence of Recombinant Human Fc gamma RIIIA/CD16a Protein, CF (Catalog # 4325-FC)?

    • The amino acid sequence is Gly17-Gln208, with a C-terminal 6-His tag, from Accession # AAH17865. This includes Tyr158 and Val176.

  2. Is Recombinant Human Fc gamma RIIIA/CD16a Protein, CF (Catalog # 4325-FC) the low binding or high binding form?

    • Catalog # 4325-FC has Valine at amino acid position 176, making it the high binding form. We also offer the low binding form, with 176F, as catalog # 8894-FC.

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Reviews for Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

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Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
By Jenna Nguyen on 03/25/2019
Application: Immunoassay Standard
Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
By VALENCIO SALEMA on 03/06/2019
Application: Binding assay/Protein-protein interaction
Binding assay/Protein-protein interaction Recombinant Human Fc gamma RIIIA/CD16a Protein, CF 4325-FC
Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
By Jenna Nguyen on 10/24/2018
Application: In vitro bioactivity in cell culture
Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
By Jenna Nguyen on 10/12/2018
Application: Enzymatic activity in vitro
Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
By Jenna Nguyen on 07/11/2018
Application: Apoptosis assay
Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
By Anonymous on 10/26/2015
Sample Tested: Human recombinant test antibody Species: Human

Specificity: Specific
Sensitivity: Sensitive
Buffer: TBS + Tween + Milk
Dilution: used at 0.25 ug/mL