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Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF

Catalog #: 8894-FC
2 Citations Datasheet / COA / SDS
Low-affinity V176F polymorphic variant
Catalog # Availability Size / Price Qty
8894-FC-050
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Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein Binding Activity
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Citations (2)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Fc gamma RIIIa/CD16a (V176F) was immobilize on a Ms x PolyHis coated plate, it binds biotinylated Human IgG. The concentration of biotinylated Human IgG that produces 50% of the optimal binding response is approximately 0.5-2.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a protein
Gly17-Gln208, with a C-terminal 6-His tag
Accession #
P08637
N-terminal Sequence
Analysis
Gly17 & Met18
Predicted Molecular Mass
23 kDa
SDS-PAGE
43-58 kDa, reducing conditions

Product Datasheets

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8894-FC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8894-FC

Formulation Lyophilized from a 0.2 μm filtered solution in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein Binding Activity View Larger

When Recombinant Human Fc gamma RIIIa/CD16a (V176F) was immobilize on a Ms x PolyHis coated plate, it binds biotinylated Human IgG with an ED50 of 0.5-2.5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIIA/CD16a

Fc gamma  RIIIA/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes (1-3). In humans, it is expressed as a 50-70 kDa transmembrane activating receptor on NK cells, T cells, monocytes, and macrophages (2). It is closely related to the GPI-linked Fc gamma RIIIB which is expressed on human neutrophils and eosinophils (1, 3). These two proteins share 97% amino acid (aa) identity within their extracellular domains (ECD) (4). The ECD of Fc gamma RIIIA also shares 63%, 61%, 65%, 59%, and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIA, feline CD16, bovine CD16, and porcine Fc gamma RIIIB, respectively. Mature human Fc gamma RIIIA consists of a 192 aa ECD with two C2-type Ig-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (5, 6). Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta (7, 8). Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA (9). Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity (10-13). Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis (13). Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases (11, 12).

References
  1. Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.
  2. Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
  3. Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
  4. Scallon, B.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5079.
  5. Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
  6. Dall’Ozzo, S. et al. (2004) Cancer Res. 64:4664.
  7. Kim, M.-K. et al. (2003) Blood 101:4479.
  8. Lanier, L.L. et al. (1989) Nature 342:803.
  9. Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.    
  10. Li, P. et al. (2007) J. Biol. Chem. 282:6210.
  11. Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
  12. Masuda, M. et al. (2006) Atherosclerosis 188:377.
  13. Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.
Long Name
Fc gamma Receptor III A
Entrez Gene IDs
2214 (Human); 304966 (Rat)
Alternate Names
CD16a antigen; CD16a; CD16FCRIIIA; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc gamma receptor III-A; Fc gamma RIIIA; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; FCRIII; FcRIIIa; IGFR3FcgammaRIIIA; immunoglobulin G Fc receptor III; low affinity III, receptor for (CD16); low affinity immunoglobulin gamma Fc region receptor III-A; neutrophil-specific antigen NA

Citations for Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Discovery of a novel Igbeta and FcgammaRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus
    Authors: Y Kitanaga, D Yamajuku, S Kubo, K Nakamura, M Maeda, M Seki, Y Kaneko, F Kinugasa, T Morokata, Y Kondo, H Yoshinari, S Nakayamada, T Sumida, Y Tanaka
    International immunopharmacology, 2021-11-12;101(0):108343.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  2. Anti-CD20 Antibody with Multimerized Fc Domains: A Novel Strategy To Deplete B Cells and Augment Treatment of Autoimmune Disease.
    Authors: Zhang X, Olsen H, Chen S, So E, Zhou H, Burch E, Merigeon E, Block D, Strome S
    J Immunol, 2015-12-22;196(3):1165-76.
    Applications: Surface Plasmon Resonance

FAQs

  1. Is Recombinant Human Fc gamma RIIIA/CD16a (V176F) Protein, CF (Catalog # 8894-FC) the low binding or high binding form?

    • Catalog # 8894-FC has Phenylalanine (F) at amino acid position 176, making it the low binding form. We also offer the high binding form, with 176V, as catalog # 4325-FC.

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