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Recombinant Human Fc gamma RIIIB/CD16b Protein, CF

Catalog #: 1597-FC/CF
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1597-FC-050/CF
Recombinant Human Fc gamma RIIIB/CD16b Protein Binding Activity
2 Images
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fc gamma RIIIB/CD16b Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind human IgG with an estimated KD <150 nM.
Source
Mouse myeloma cell line, NS0-derived human Fc gamma RIIIB/CD16b protein
Thr20-Gln208, with a C-terminal 10-His tag
Accession #
O75015
N-terminal Sequence
Analysis
Thr20
Predicted Molecular Mass
22.7 kDa
SDS-PAGE
40-60 kDa, reducing conditions

Product Datasheets

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1597-FC/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1597-FC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Fc gamma RIIIB/CD16b Protein Binding Activity View Larger

Recombinant Human Fc gamma RIIIB/CD16b (Catalog # 1597-FC/CF) binds human IgG with an estimated Kd<150 nM.

SDS-PAGE Recombinant Human Fc gamma RIIIB/CD16b Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human Fc gamma RIIIB/CD16b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 43-60 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIIB/CD16b

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta  chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Signaling through Fc gamma RIIIA results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells. By contrast, Fc gamma RIIIB is a decoy receptor that binds IgG complexes without triggering activation. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes (4). It interacts with complement receptors CR3 and CR4 on monocytes to induce the production of pro-inflammatory cytokines.

References
  1. van de Winkel, J, and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Gauchat, G.J. et al. (1996) J. Immunol. 157:1184.
Long Name
Fc gamma Receptor III
Entrez Gene IDs
2215 (Human); 14131 (Mouse)
Alternate Names
CD16; CD16b antigen; CD16b; Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc fragment of IgG, low affinity IIIb, receptor for (CD16); Fc gamma RIIIB; FCG3; Fc-gamma receptor IIIb (CD 16); Fc-gamma RIII; Fc-gamma RIIIb; Fc-gamma RIII-beta; FCGR3; FCGR3B; FcgRIIIB; FcR-10; fcRIII; FCRIIIB; IGFR3; IgG Fc receptor III-1; low affinity immunoglobulin gamma Fc region receptor III-B

Citations for Recombinant Human Fc gamma RIIIB/CD16b Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Discovery of a novel Igbeta and FcgammaRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus
    Authors: Y Kitanaga, D Yamajuku, S Kubo, K Nakamura, M Maeda, M Seki, Y Kaneko, F Kinugasa, T Morokata, Y Kondo, H Yoshinari, S Nakayamada, T Sumida, Y Tanaka
    International immunopharmacology, 2021-11-12;101(0):108343.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  2. Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus
    Authors: PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass
    J. Immunol., 2019-01-25;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  3. Analytical and functional similarity of Amgen biosimilar ABP 215 to bevacizumab
    Authors: N Seo, A Polozova, M Zhang, Z Yates, S Cao, H Li, S Kuhns, G Maher, HJ McBride, J Liu
    MAbs, 2018-04-20;0(0):1-58.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  4. Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases.
    Authors: Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A
    J Immunol, 2014-03-26;192(9):4083-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  5. Increasing FcgammaRIIa affinity of an FcgammaRIII-optimized anti-EGFR antibody restores neutrophil-mediated cytotoxicity.
    Authors: Derer S, Glorius P, Schlaeth M, Lohse S, Klausz K, Muchhal U, Desjarlais J, Humpe A, Valerius T, Peipp M
    MAbs, 2013-12-11;6(2):409-21.
    Applications: Surface Plasmon Resonance
  6. Bi-specific Aptamers Mediating Tumor Cell Lysis.
    Authors: Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B
    J. Biol. Chem., 2011-04-29;286(24):21896-905.
    Species: Human
    Sample Types: DNA
    Applications: EMSA
  7. Alpha-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8.
    Authors: Bergin DA, Reeves EP, Meleady P, Henry M, McElvaney OJ, Carroll TP, Condron C, Chotirmall SH, Clynes M, O'Neill SJ, McElvaney NG
    J. Clin. Invest., 2010-11-08;120(12):4236-50.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)

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