Recombinant Human FGF basic/FGF2/bFGF Protein, Animal-Free

Name: Recombinant Human FGF basic/FGF2/bFGF Protein, Animal-Free
Brand: R&D Systems
SKU: BT-FGFB-AFL
Price: 1587 USD

Catalog #: BT-FGFB-AFL Datasheet / COA / SDS

GMP Version Available: BT-FGFB-GMP

GMP

Intended for preclinical researchers who may transition to GMP FGF basic/FGF2 for their clinical work

Catalog #	Availability	Size / Price	Qty
BT-FGFB-AFL-01M
BT-FGFB-AFL-025

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Animal-Free™ Recombinant Human FGF basic/FGF2/bFGF Protein Lot-to-Lot Consistency.

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All FGF basic/FGF2/bFGF Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF basic/FGF2/bFGF Protein, Animal-Free Summary

Learn more about Animal-Free Recombinant Proteins

Product Specifications

Purity

>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 0.100-1.00 ng/mL.The specific activity of Recombinant Human FGF basic/FGF2 is >8.0 x 10⁵ IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).

Source

E. coli-derived human FGF basic/FGF2/bFGF protein
Ala135-Ser288
Produced using non-animal reagents in an animal-free laboratory.

Accession #

NP_001997.5

N-terminal Sequence
Analysis

Ala135 & Ala136

Predicted Molecular Mass

17 kDa

SDS-PAGE

18 kDa, under reducing conditions.

Product Datasheets

BT-FGFB-AFL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-FGFB-AFL

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose.
Reconstitution	Reconstitute 25 μg size at 250 μg/mL and others at 500 μg/mL in sterile water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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The lot-to-lot consistency of Animal-Free™ Recombinant Human FGF basic/FGF2/bFGF Protein (Catalog # BT-FGFB-AFL) was assessed by testing the ability of two independent lots of the protein to stimulate proliferation of the NR6R-3T3 mouse fibroblast cell line. The ED₅₀ for this effect is 0.100-1.00 ng/mL.

SDS-PAGE

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2 μg/lane of Animal-Free™ Recombinant Human FGF basic/FGF2/bFGF Protein (Catalog # BT-FGFB-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18 kDa.

Reconstitution Calculator

Background: FGF basic/FGF2/bFGF

FGF basic (also known as FGF-2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF-2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4-6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).

References

Sorenson, V. et al. (2006) BioEssays 28:504.
Kardami, E. et al. (2004) Cardiovasc. Res. 63:458.
Nugent, M.A. and R.V. Lozzo (2000) Int. J. Biochem. Cell Biol. 32:115.
Abraham, J.A. et al. (1986) EMBO J. 5:2523.
Prats, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:1836.
Arnaud, E. et al. (1999) Mol. Cell. Biol. 19:505.
Foletti, A. et al. (2003) Cell. Mol. Life Sci. 60:2254.
Arese, M. et al. (1999) Mol. Biol. Cell 10:1429.
Pintucci, G. et al. (1996) Mol. Biol. Cell 7:1249.
Nickel, W. (2005) Traffic 6:607.
SwissProt # P09038.
Klagsbrun, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1839.
Bailly, K. et al. (2000) FASEB J. 14:333.
Hebert, J.M. et al. (1990) Dev. Biol. 138:454.

Long Name

Fibroblast Growth Factor basic

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Alternate Names

basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement