Recombinant Human FGFR4 Fc Chimera Avi-tag Protein, CF

Catalog #: AVI685 Datasheet / COA / SDS

Biotinylated

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AVI685-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGFR4 Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. In a Human FGF acidic/FGF1 antibody (Catalog # AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (Catalog # 232-FA), Biotinylated Recombinant Human FGFR4 Fc Chimera Avi-tag Protein binds with an ED₅₀ of 50.0‑400 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human FGFR4 protein

Human FGFR4 (Leu22-Asp369) Accession # P22455.2	IEGRMD	Human IgG₁ Fc (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P22455.2

N-terminal Sequence
Analysis

Leu22

Structure / Form

Disulfide-linked homodimer

Biotinylated via Avi-tag

Predicted Molecular Mass

67 kDa

SDS-PAGE

90-105 kDa, under reducing conditions.

Product Datasheets

AVI685

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI685

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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In a Human FGF acidic/FGF1 antibody (AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (232-FA), Biotinylated Recombinant Human FGFR4 Fc Chimera Avi-tag Protein (Catalog # AVI685) binds with an ED₅₀ of 50.0-400 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human FGFR4 Fc Chimera Avi-tag Protein (Catalog # AVI685) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-105 kDa and 180-210 kDa, respectively.

Reconstitution Calculator

Background: FGFR4

Fibroblast growth factor receptor 4 (FGFR4) belongs to a family of type I transmembrane tyrosine kinases which mediate the biological functions of FGFs that are involved in a multitude of physiological and pathological cellular processes (1). The FGFR family is comprised of 4 structurally conserved members (FGFR1-4) all possessing and extracellular domain (ECD) with three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain (1, 2). The ECD of mature human FGFR4 shares 90% amino acid sequence identity with mouse FGFR4. Alternative splicing of the IgIII domain generates multiple forms of FGFR13, but FGFR4 does not have a splice variant (3, 4). FGFR4 exhibits distinct and varying binding affinities for different FGF ligands, with FGF1, FGF4, and FGF8 showing the highest affinity (4). FGFRs mediate the FGF signaling cascade which regulate developmental processes including cellular proliferation, differentiation, and migration, morphogenesis, and patterning (5). FGFRs transduce the signals through three dominant pathways including RAS/MAPK, PI3k/AKT, and PLC gamma (6). FGFR4 is expressed at high levels during embryonic development and is required for the maintenance of both lipid and glucose metabolism as well as an established role in cholesterol metabolism (7). Overexpression of the FGFR4 has been reported in several solid tumors including breast cancer, prostate cancer, pancreatic cancer, and renal cell carcinoma (4, 8). Further, FGFR4 expression is significantly upregulated in most liver cancer cases, and enhanced FGF19-FGFR4 signaling is linked to hepatocellular carcinoma progression, metastasis, and poor survival (8). FGFR4 is being explored as a potential therapeutic target for breast cancer and other solid tumors (9). Our Avi-tag Biotinylated FGFR4 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Ornitz, D.M. and Itoh, N. (2015) Wiley Interdiscip Rev. Dev. Biol. 4:215.
Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
Ferguson, H.R. et al. (2021) Signaling. Cells 10:1201.
Lang, L. and Teng, Y. (2019) Cells. 8:31.
Xie, Y. et al. (2020) Sig. Transduct Target Ther 5:181.
Mossahebi-Mohammadi, M. et al. (2020) Front Cell Dev Biol. 18:79.
Huang, X. et al. (2007) Diabetes 56:2501.
Liu, Y. et al. (2020) Front Cell Dev Biol. 8:95.
Levine, K.M. et al. (2020) Pharmacol Ther. 214:107590.

Long Name

Fibroblast Growth Factor Receptor 4

Entrez Gene IDs

2264 (Human); 14186 (Mouse)

Alternate Names

CD334 antigen; CD334; EC 2.7.10; EC 2.7.10.1; FGF R4; FGFR4; FGFR-4; fibroblast growth factor receptor 4; JTK2hydroxyaryl-protein kinase; MGC20292; protein-tyrosine kinase; TKF; tyrosine kinase related to fibroblast growth factor receptor; tyrosylprotein kinase