Home / Gas1 / Recombinant Human Gas1 Protein, CF

Recombinant Human Gas1 Protein, CF

Catalog #: 2636-GS
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2636-GS-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Gas1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhGas1 at 1 µg/mL (100 µL/well) can bind rmArtemin at a linear range of 0.1-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Gas1 protein
Leu40-Ser318, with a C-terminal 6-His tag
Accession #
P54826
N-terminal Sequence
Analysis
Leu40
Structure / Form
Monomer
Predicted Molecular Mass
30.1 kDa
SDS-PAGE
40 kDa, reducing conditions

Product Datasheets

You must select a language.

x

2636-GS

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2636-GS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Gas1

Gas1 (Growth Arrest Specific 1) is one of six structurally unrelated proteins that were identified by their increased expression in growth-arrested cells relative to actively proliferating cells (1, 2). Following mitogenic stimulation, Gas1 expression is transcriptionally suppressed by c-Myc as cells transit from G0 to G1 phases of the cell cycle (3, 4). Overexpression of Gas1 prevents S phase entry and DNA synthesis (5). Gas1-mediated blockade of the cell cycle is p53-dependent but does not require the transactivating domain of p53 (6). The human Gas1 cDNA encodes a 345 amino acid (aa) precursor that includes a 39 aa signal sequence, a 279 aa mature protein, and a 27 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-rich regions as well as an RGD sequence (5). Mature human and mouse Gas1 share 85% aa sequence identity. Human Gas1 is a 40 kDa GPI-linked glycoprotein that is uniformly distributed on the cell surface (7). In contact-inhibited vascular endothelial cells, Gas1 is induced by VE-Cadherin and VEGF expression and mediates the anti-apoptotic effect of VEGF (8). In contrast, Gas1 is induced in hippocampal neurons after NMDA exposure but functions as a pro-apoptotic effector of NMDA-mediated excitotoxicity (9). Gas1 exhibits a range of developmental actions including either promoting or inhibiting growth and differentiation of somite, limb, cerebellar, and eye tissues (10 - 14). Gas1 contributes to the antagonistic effect of Wnt proteins toward Shh function by binding the N-terminal region of Shh (11). The dependence of Gas1 function on the cellular context has been addressed by suggesting that Gas1 could function as a co-receptor for GDNF family ligands (15). This speculation is supported by R&D Systems data which demonstrate direct binding of Gas1 to Artemin and Neurturin.

References
  1. Schneider, C. et al. (1988) Cell 54:787.
  2. Mullor, J.L. and A.R. Altaba (2002) BioEssays 24:22.
  3. Del Sal, G. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1848.
  4. Lee, T.C. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12886.
  5. Del Sal, G. et al. (1992) Cell 70:595.
  6. Del Sal, G. et al. (1995) Mol. Cell. Biol. 15:7152.
  7. Stebel, M. et al. (2000) FEBS Lett. 481:152.
  8. Spagnuolo, R. et al. (2004) Blood 103:3005.
  9. Mellstrom, B. et al. (2002) Mol. Cell Neurosci. 19:417.
  10. Lee, K.K.H. et al. (2001) Dev. Biol. 234:188.
  11. Lee, C.S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11347.
  12. Liu, Y. et al. (2002) Development 129:5289.
  13. Liu, Y. et al. (2001) Dev. Biol. 236:30.
  14. Lee, C.S. et al. (2001) Dev. Biol. 236:17.
  15. Schueler-Furman, O. et al. (2006) Trends Pharmacol. Sci. 27:72.
Long Name
Growth-arrest-specific Protein 1
Entrez Gene IDs
2619 (Human); 14451 (Mouse)
Alternate Names
Gas1; GAS-1; growth arrest-specific 1; Growth arrest-specific gene-1; growth arrest-specific protein 1

Citation for Recombinant Human Gas1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Growth arrest-specific protein 1 is a novel endogenous inhibitor of glomerular cell activation and proliferation.
    Authors: van Roeyen C, Zok S, Pruessmeyer J, Boor P, Nagayama Y, Fleckenstein S, Cohen C, Eitner F, Grone H, Ostendorf T, Ludwig A, Floege J
    Kidney Int, 2012-12-19;83(2):251-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Loading...

Reviews for Recombinant Human Gas1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Gas1 Protein, CF and earn rewards!

Have you used Recombinant Human Gas1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review