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Recombinant Human Glypican 4 Fc Chimera Protein, CF

Catalog #: 9195-GP
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9195-GP-050
Recombinant Human Glypican 4 Fc Chimera Protein Bioactivity
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Product Details
Citations (4)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Glypican 4 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Glypican 4 Fc Chimera is coated at 500 ng/mL (100 μL/well), the concentration of recombinant human FGF basic that produces 50% of the optimal binding response is 0.75-4.5 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Glypican 4 protein
Human Glypican 4
(Ala23-Ser529)
Accession # O75487		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
O75487
N-terminal Sequence
Analysis
Ala23 & Ser355
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
84 kDa

Product Datasheets

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9195-GP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9195-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human Glypican 4 Fc Chimera Protein Bioactivity View Larger

When Recombinant Human Glypican 4 Fc Chimera (Catalog # 9195-GP) is coated at 500 ng/mL 100 µL/well), the concentration of Recombinant Human FGF basic 146 aa (Catalog # 233-FB) that produces 50% of the optimal binding response is 0.75-4.5 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glypican 4

Glypican 4, also known as K-Glypican, is an approximately 220 kDa GPI-anchored heparan sulfate proteoglycan with a 60 kDa protein core (1, 2). It is expressed in brain, kidney, adrenal gland, and fat tissue (1, 3) and binds to basic FGF (2). Mature human Glypican 4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat Glypican 4, respectively. An alternative splice isoform lacks the N-terminal 70 aa including the signal peptide. In the developing brain, Glypican 4 is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors (1, 2). It inhibits the dopaminergic differentiation of neurons (4). A 30 kDa cleaved form of Glypican 4 binds in cis to PTP sigma and contributes to excitatory synapse development and function (5). Glypican 4 is differentially expressed between adipose tissue depots (3). A soluble form can be released by adipocytes and circulates at elevated levels in obese patients with insulin resistance (3, 6). This form binds and enhances signaling through the Insulin R, and it also supports adipocyte differentiation (3). In zebrafish, Glypican 4 is required for cartilage formation and cardiomyocyte differentiation (7, 8).

References
  1. Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
  2. Hagihara, K. et al. (2000) Dev. Dyn. 219:353.
  3. Ussar, S. et al. (2012) Diabetes 61:2289.
  4. Fico, A. et al. (2014) J. Neurosci. 34:8318.
  5. Ko, J.S. et al. (2015) Proc. Natl. Acad. Sci. USA 112:1874.
  6. Zhu, H.J. et al. (2014) J. Endocrinol. Metab. 99:E2697.
  7. Sisson, B.E. et al. (2015) Mech. Dev. 138:279.
  8. Strate, I. et al. (2015) Development 142:1767.
Entrez Gene IDs
2239 (Human); 14735 (Mouse); 317322 (Rat)
Alternate Names
Glypican 4; glypican proteoglycan 4; glypican-4; GPC4; K-glypican; K-glypicandJ900E8.1 (glypican 4)

Citations for Recombinant Human Glypican 4 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Identification of the growth cone as a probe and driver of neuronal migration in the injured brain
    Authors: Nakajima, C;Sawada, M;Umeda, E;Takagi, Y;Nakashima, N;Kuboyama, K;Kaneko, N;Yamamoto, S;Nakamura, H;Shimada, N;Nakamura, K;Matsuno, K;Uesugi, S;Vep?ek, NA;Küllmer, F;Nasufovi?, V;Uchiyama, H;Nakada, M;Otsuka, Y;Ito, Y;Herranz-Pérez, V;García-Verdugo, JM;Ohno, N;Arndt, HD;Trauner, D;Tabata, Y;Igarashi, M;Sawamoto, K;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The IgG4 hinge with CD28 transmembrane domain improves VHH-based CAR T cells targeting a membrane-distal epitope of GPC1 in pancreatic cancer
    Authors: N Li, A Quan, D Li, J Pan, H Ren, G Hoeltzel, N de Val, D Ashworth, W Ni, J Zhou, S Mackay, SM Hewitt, R Cachau, M Ho
    Nature Communications, 2023-04-08;14(1):1986.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  3. Investigation of Glypican-4 and -6 by Infrared Spectral Imaging during the Hair Growth Cycle
    Authors: C Colin-Pier, V Untereiner, GD Sockalingu, L Ramont, S Brézillon
    International Journal of Molecular Sciences, 2023-02-21;24(5):.
    Species: N/A
    Sample Types: Buffer
    Applications: Infrared Spectral Imaging
  4. Cocaine preference and neuroadaptations are maintained by astrocytic NMDA receptors in the nucleus accumbens
    Authors: GP Shelkar, PJ Gandhi, J Liu, SM Dravid
    Science Advances, 2022-07-22;8(29):eabo6574.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay

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