Recombinant Human IGF-II/IGF2 Protein, CF Summary
Product Specifications
Ala25-Glu91
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
292-G2
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetic Acid. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
Recombinant Human IGF-II/IGF2 (Catalog # 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 1.5‑6 ng/mL.
1 μg/lane of Recombinant Human IGF-II/IGF2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Reconstitution Calculator
Background: IGF-II/IGF2
Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine and porcine proteins) and exhibit cross-species activity.
IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development.
Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally related to the insulin receptor. It is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation-independent mannose 6-phosphate receptor. At the present time, it is not known if the type II IGF receptor participates in the IGF signaling pathway. An additional unknown receptor which mediates IGF‑II signaling has also been proposed. Circulating IGFs exist in complexes bound to IGF binding proteins. Currently, at least six high affinity binding proteins have been identified.
Citations for Recombinant Human IGF-II/IGF2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Skeletal overgrowth in a pre-pubescent child treated with pan-FGFR inhibitor
Authors: Majlessipour, F;Zhu, G;Baca, N;Kumbaji, M;Hwa, V;Danielpour, M;
Heliyon
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Endothelial cell Piezo1 promotes vascular smooth muscle cell differentiation on large arteries
Authors: Abello, J;Yin, Y;Zhao, Y;Maurer, J;Lee, J;Bodell, C;Clevenger, AJ;Burton, Z;Goeckel, ME;Lin, M;Grainger, S;Halabi, CM;Raghavan, SA;Sah, R;Stratman, AN;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Regulation of angiogenesis by endocytic trafficking mediated by cytoplasmic dynein 1 light intermediate chain 1
Authors: Johnson, D;Colijn, S;Richee, J;Yano, J;Burns, M;Davis, AE;Pham, VN;Saric, A;Jain, A;Yin, Y;Castranova, D;Melani, M;Fujita, M;Grainger, S;Bonifacino, JS;Weinstein, BM;Stratman, AN;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CXCR3-CXCL11 signaling restricts angiogenesis and promotes pericyte recruitment
Authors: Goeckel, ME;Lee, J;Levitas, A;Colijn, S;Mun, G;Burton, Z;Chintalapati, B;Yin, Y;Abello, J;Stratman, A;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
The Role of SOX9 in IGF-II-Mediated Pulmonary Fibrosis
Authors: Waldrep, KM;Rodgers, JI;Garrett, SM;Wolf, BJ;Feghali-Bostwick, CA;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Bioassay -
Senescence induces proteolytically-active PAPP-A secretion and association with extracellular vesicles in human pre-adipocytes
Authors: CA Conover, LK Bale
Experimental gerontology, 2022-12-20;172(0):112070.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The insulin and IGF signaling pathway sustains breast cancer stem cells by IRS2/PI3K-mediated regulation of MYC
Authors: JS Lee, MW Lero, J Mercado-Ma, S Zhu, M Jo, CE Tocheny, JS Morgan, LM Shaw
Cell Reports, 2022-12-06;41(10):111759.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells
Authors: S Funakoshi, I Fernandes, O Mastikhina, D Wilkinson, T Tran, W Dhahri, A Mazine, D Yang, B Burnett, J Lee, S Protze, GD Bader, SS Nunes, M Laflamme, G Keller
Nature Communications, 2021-05-26;12(1):3155.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mesenchyme-derived IGF2 is a major paracrine regulator of pancreatic growth and function
Authors: CM Hammerle, I Sandovici, GV Brierley, NM Smith, WE Zimmer, I Zvetkova, HM Prosser, Y Sekita, BYH Lam, M Ma, WN Cooper, A Vidal-Puig, SE Ozanne, G Medina-Góm, M Constância
PLoS Genet, 2020-10-15;16(10):e1009069.
Species: Mouse
Sample Types: Serum
Applications: Western Blot -
Intracellular Insulin-like growth factor binding protein 2 (IGFBP2) contributes to the senescence of keratinocytes in psoriasis by stabilizing cytoplasmic p21
Authors: L Mercurio, D Lulli, F Mascia, E Dellambra, C Scarponi, M Morelli, C Valente, ML Carbone, S Pallotta, G Girolomoni, C Albanesi, S Pastore, S Madonna
Aging (Albany NY), 2020-04-17;12(8):6823-6851.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Marked structural rearrangement of mannose 6-phosphate/IGF2 receptor at different pH environments
Authors: R Wang, X Qi, P Schmiege, E Coutavas, X Li
Sci Adv, 2020-02-12;6(7):eaaz1466.
Species: Human
Sample Types: Protein
Applications: Binding Assay -
Target prediction and validation of microRNAs expressed from FSHR and aromatase genes in human ovarian granulosa cells
Authors: I Rooda, K Hensen, B Kaselt, S Kasvandik, M Pook, A Kurg, A Salumets, A Velthut-Me
Sci Rep, 2020-02-10;10(1):2300.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Insulin-like growth factor (IGF)-II- mediated fibrosis in pathogenic lung conditions
Authors: SM Garrett, E Hsu, JM Thomas, JM Pilewski, C Feghali-Bo
PLoS ONE, 2019-11-25;14(11):e0225422.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Systematic assessment of antibody selectivity in plasma based on a resource of enrichment profiles
Authors: C Fredolini, S Byström, L Sanchez-Ri, M Ioannou, D Tamburro, F Pontén, RM Branca, P Nilsson, J Lehtiö, JM Schwenk
Sci Rep, 2019-06-06;9(1):8324.
Species: Human
Sample Types: Plasma
Applications: ELISA Capture -
Immune targeting of autocrine IGF2 hampers rhabdomyosarcoma growth and metastasis
Authors: C De Giovann, P Nanni, L Landuzzi, ML Ianzano, G Nicoletti, S Croci, A Palladini, PL Lollini
BMC Cancer, 2019-02-07;19(1):126.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA (Capture), Western Blot -
Cellular characterization of human epicardial adipose tissue: highly expressed PAPP-A regulates insulin-like growth factor I signaling in human cardiomyocytes
Authors: CA Conover, LK Bale, RL Frye, HV Schaff
Physiol Rep, 2019-02-01;7(4):e14006.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay -
A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells
Authors: Z Chen, J Liu, D Chu, Y Shan, G Ma, H Zhang, XD Zhang, P Wang, Q Chen, C Deng, W Chen, DS Dimitrov, Q Zhao
Int. J. Biol. Sci., 2018-05-21;14(7):799-806.
Species: Human
Sample Types: Whole Cells
Applications: Library Screening -
Generation of hepatocyte- and endocrine pancreatic-like cells from human induced endodermal progenitor cells
Authors: R Sambathkum, R Akkerman, S Dastidar, P Roelandt, M Kumar, M Bajaj, AR Mestre Ros, N Helsen, V Vanslembro, E Kalo, S Khurana, J Laureys, C Gysemans, MM Faas, P de Vos, CM Verfaillie
PLoS ONE, 2018-05-11;13(5):e0197046.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
Proc. Natl. Acad. Sci. U.S.A., 2018-01-16;0(0):.
Applications: Bioassay -
Matrix metalloproteinase-1 facilitates MSC migration via cleavage of IGF-2/IGFBP2 complex
Authors: SP Guan, ATL Lam, JP Newman, KLM Chua, CYL Kok, ST Chong, MLK Chua, PYP Lam
FEBS Open Bio, 2017-11-23;8(1):15-26.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CD44(+) fibroblasts increases breast cancer cell survival and drug resistance via IGF2BP3-CD44-IGF2 signalling
Authors: Y Liu, C Yu, Y Wu, X Sun, Q Su, C You, H Xin
J. Cell. Mol. Med., 2017-05-18;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay, ELISA (Standard) -
Mural-Endothelial cell-cell interactions stabilize the developing zebrafish dorsal aorta
Authors: Amber N Stratman
Development, 2016-12-02;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TGF? signaling confers sorafenib resistance via induction of multiple RTKs in hepatocellular carcinoma cells
Authors: Tong Wu
Mol. Carcinog., 2016-11-25;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Addiction to the IGF2-ID1-IGF2 circuit for maintenance of the breast cancer stem-like cells
Oncogene, 2016-08-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Antitumor efficacy of the heparan sulfate mimic roneparstat (SST0001) against sarcoma models involves multi-target inhibition of receptor tyrosine kinases
Authors: G Cassinelli, E Favini, L Dal Bo, M Tortoreto, M De Maglie, G Dagrada, S Pilotti, F Zunino, N Zaffaroni, C Lanzi
Oncotarget, 2016-07-26;7(30):47848-47863.
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Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS
Sci Rep, 2016-05-16;6(0):25960.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate.
Authors: Gomez D, O'Driscoll M, Sheets T, Hruban R, Oberholzer J, McGarrigle J, Shamblott M
PLoS ONE, 2015-08-19;10(8):e0133862.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Enhanced memory consolidation in mice lacking the circadian modulators Sharp1 and -2 caused by elevated Igf2 signaling in the cortex.
Authors: Shahmoradi A, Radyushkin K, Rossner M
Proc Natl Acad Sci U S A, 2015-06-22;112(27):E3582-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA (Standard) -
Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
Authors: Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, Nolan D, Elemento O, Wood B, Adair J, Rafii S, Kiem H
J Clin Invest, 2015-02-09;125(3):1243-54.
Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
Sample Types: Whole Cells
Applications: Bioassay -
IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
Reprod Biol Endocrinol, 2014-12-04;12(0):123.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A positive autoregulatory BDNF feedback loop via C/EBPbeta mediates hippocampal memory consolidation.
Authors: Bambah-Mukku D, Travaglia A, Chen D, Pollonini G, Alberini C
J Neurosci, 2014-09-10;34(37):12547-59.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
Br J Cancer, 2014-05-22;110(12):2865-73.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The IGF2 mRNA binding protein p62/IGF2BP2-2 induces fatty acid elongation as a critical feature of steatosis.
Authors: Laggai S, Kessler S, Boettcher S, Lebrun V, Gemperlein K, Lederer E, Leclercq I, Mueller R, Hartmann R, Haybaeck J, Kiemer A
J Lipid Res, 2014-04-22;55(6):1087-1097.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Insulin-like growth factor 2 enhances regulatory T-cell functions and suppresses food allergy in an experimental model.
Authors: Yang G, Geng X, Song J, Wu Y, Yan H, Zhan Z, Yang L, He W, Liu Z, Qiu S, Liu Z, Yang P
J Allergy Clin Immunol, 2014-04-01;133(6):1702-8.e5.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Insulin-like growth factor II peptide fusion enables uptake and lysosomal delivery of alpha-N-acetylglucosaminidase to mucopolysaccharidosis type IIIB fibroblasts.
Authors: Kan S, Troitskaya L, Sinow C, Haitz K, Todd A, Di Stefano A, Le S, Dickson P, Tippin B
Biochem J, 2014-03-01;458(2):281-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells.
Authors: Cai, Qing, Bonfanti, Paola, Sambathkumar, Rangaraj, Vanuytsel, Kim, Vanhove, Jolien, Gysemans, Conny, Debiec-Rychter, Maria, Raitano, Susanna, Heimberg, Harry, Ordovas, Laura, Verfaillie, Catherin
Stem Cells Transl Med, 2014-02-03;3(4):489-99.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IGFBP7 participates in the reciprocal interaction between acute lymphoblastic leukemia and BM stromal cells and in leukemia resistance to asparaginase.
Authors: Laranjeira AB, de Vasconcellos JF, Sodek L, Spago MC, Fornazim MC, Tone LG, Brandalise SR, Nowill AE, Yunes JA
Leukemia, 2011-10-18;26(5):1001-11.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Kallikrein-related peptidase 12 hydrolyzes matricellular proteins of the CCN family and modifies interactions of CCN1 and CCN5 with growth factors.
Authors: Guillon-Munos A, Oikonomopoulou K, Michel N, Smith CR, Petit-Courty A, Canepa S, Reverdiau P, Heuzé-Vourc'h N, Diamandis EP, Courty Y
J. Biol. Chem., 2011-05-31;286(29):25505-18.
Applications: Enzyme Assay Substrate -
A novel type of glial cell in the retina is stimulated by insulin-like growth factor 1 and may exacerbate damage to neurons and Muller glia.
Authors: Fischer AJ, Scott MA, Zelinka C, Sherwood P
Glia, 2010-04-15;58(6):633-49.
Species: Chicken
Sample Types: In Vivo
Applications: In Vivo -
CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis.
Authors: Han Y, Cui J, Tao J, Guo L, Guo P, Sun M, Kang J, Zhang X, Yan C, Li S
Exp. Cell Res., 2009-09-19;315(19):3301-11.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases.
Authors: Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW
Proc. Natl. Acad. Sci. U.S.A., 2009-05-29;106(24):9826-30.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Insulin-like growth factor-I receptor blockade by a specific tyrosine kinase inhibitor for human gastrointestinal carcinomas.
Authors: Piao W, Wang Y, Adachi Y, Yamamoto H, Li R, Imsumran A, Li H, Maehata T, Ii M, Arimura Y, Lee CT, Shinomura Y, Carbone DP, Imai K
Mol. Cancer Ther., 2008-06-01;7(6):1483-93.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Insulin-like growth factor-II is increased in systemic sclerosis-associated pulmonary fibrosis and contributes to the fibrotic process via Jun N-terminal kinase- and phosphatidylinositol-3 kinase-dependent pathways.
Authors: Hsu E, Feghali-Bostwick CA
Am. J. Pathol., 2008-05-08;172(6):1580-90.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Association between paternally inherited haplotypes upstream of the insulin gene and umbilical cord IGF-II levels.
Authors: Adkins RM, Fain JN, Krushkal J
Pediatr. Res., 2007-10-01;62(4):451-5.
Applications: ELISA (Standard) -
Insulin-like growth factor (IGF) 2 stimulates steroidogenesis and mitosis of bovine granulosa cells through the IGF1 receptor: role of follicle-stimulating hormone and IGF2 receptor.
Authors: Spicer LJ, Aad PY
Biol. Reprod., 2007-03-14;77(1):18-27.
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay -
Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.
Authors: Hiroyama T, Miharada K, Aoki N, Fujioka T, Sudo K, Danjo I, Nagasawa T, Nakamura Y
Exp. Hematol., 2006-06-01;34(6):760-9.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Cells
Applications: Bioassay -
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology, 2006-03-06;130(6):1754-63.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability.
Authors: Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A
Biochem. Biophys. Res. Commun., 2005-08-05;333(3):1011-6.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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Cells were seeded in 12-well plates and grown to confluence. Culture medium was replaced with 0.5 ml of EMEM without serum prior to the addition of uptake inhibitors. 5 µg/ml recombinant human IGF-II (R&D Systems; Minneapolis, MN) was applied to the cells for 10 minutes prior to applying purified rhNAGLU-IGF-II at a final concentration of 160 units/ml. Following 4 hours incubation at 37°C and 5% CO2, cells were harvested and intracellular NAGLU activity was measured as described above. The enzyme activity measured in MPS IIIB fibroblasts with rhNAGLU-IGF-II treatment alone was defined as 100% and the intracellular enzyme activity observed in the presence of inhibitor was normalized to rhNAGLU-IGF-II activity. (Biochem J. 2014 458(2):281-9.)