Recombinant Human IGFBP-4 Protein, CF Summary
Product Specifications
Asp22-Glu258, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
804-GB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IGFBP-4
IGFBP-4 (insulin-like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins that maintain IGF-I and IGF-II in the circulation and direct them to their target tissues (1, 2). Human IGFBP-4 cDNA encodes 258 amino acids (aa) that include a 21 aa signal peptide and a 237 aa cysteine-rich mature protein that contains an N-terminal IGFBP domain, a linker sequence, and a C-terminal thyroglobulin domain. Mature human IGFBP-4 shares 91% aa sequence identity with mouse and rat IGFBP-4, and 97% with canine, bovine, ovine and porcine IGFBP-4. Both domains interact with IGFs, although the N‑terminal domain is primary (2). The linker contains a cleavage site that is accessible by PAPP-A (pregnancy-associated plasma protein A) when IGFs are bound (2, 3). IGFBP-4 fragments detected in the plasma of pregnant women have lowered affinity for IGFs, thus upregulating IGF bioavailability for receptor binding (2). Unlike other family members, IGFBP-4 does not interact with the extracellular matrix or translocate to the nucleus (2). Like other family members, it is expressed early in development and has both IGF-dependent and -independent activities (2, 4). IGFBP-4 expression in mesenchymal stem cells is upregulated by the cardiac transcription factor GATA-4 (5). It then enhances cardiomyocyte differentiation by interacting with Frizzled and LRP proteins, inhibiting canonical Wnt signaling (6, 7). IGF can sequester IGFBP-4 when present, inhibiting its ability to support cardiomyocyte differentiation (3, 4). IGFBP-4 is also upregulated in osteoblasts in response to estrogen (8). Intact or proteolytically cleaved IGFBP-4 may either promote or inhibit cancer cell growth, motility and invasion depending on cell type, tissue and stage of the cancer (9-11). It is shown to inhibit IGF-1- or FGF-2-induced angiogenesis, but not that induced by VEGF (10).
- Bethel, C.R. et al. (1994) Biochem. Mol. Biol. Int. 34:385.
- Forbes, B.E. et al. (2012) Front. Endocrinol. (Lausanne) 3:38.
- Gyrup, C. and C. Oxvig (2007) Biochemistry 46:1972.
- Schuller, A.G. et al. (1994) Mol. Cell. Endocrinol. 104:57.
- Li, H. et al. (2011) Cytotherapy 13:1057.
- Zhu, W. et al. (2008) Nature 454:345.
- Minato, A. et al. (2012) Biomaterials 33:515.
- Denger, S. et al. (2008) Mol. Endocrinol. 22:361.
- Ueno, K. et al. (2011) Int. J. Cancer 129:2360.
- Contois, L.W. et al. (2012) J. Biol. Chem. 287:1779.
- Moreno, M.J. et al. (2013) Neoplasia 15:554.
Citations for Recombinant Human IGFBP-4 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition
Authors: RA Judge, J Sridar, K Tunyasuvun, R Jain, JCK Wang, C Ouch, J Xu, A Mafi, AH Nile, C Remarcik, CL Smith, C Ghosh, C Xu, V Stoll, J Jumper, AH Singh, D Eaton, Q Hao
Nature Communications, 2022-09-20;13(1):5500.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Inhibition of longevity regulator PAPP-A modulates tissue homeostasis via restraint of mesenchymal stromal cells
Authors: M Mohrin, J Liu, J Zavala-Sol, S Bhargava, J Maxwell Tr, A Brito, D Hu, D Brooks, G Koukos, L Alabdulaal, JS Paw, K Hake, G Kolumam, ML Bouxsein, R Baron, Y Kutskova, A Freund
Aging Cell, 2021-02-09;0(0):e13313.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Effects of Transport Duration and Environmental Conditions in Winter or Summer on the Concentrations of Insulin-Like Growth Factors and Insulin-Like Growth Factor-Binding Proteins in the Plasma of Market-Weight Pigs
Authors: E Wirthgen, S Goumon, M Kunze, C Walz, M Spitschak, A Tuchschere, J Brown, C Höflich, L Faucitano, A Hoeflich
Front Endocrinol (Lausanne), 2018-02-13;9(0):36.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
Reprod Biol Endocrinol, 2014-12-04;12(0):123.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
Cancer Res., 2009-03-24;69(7):2996-3003.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development.
Authors: Wang TH, Chang CL, Wu HM, Chiu YM, Chen CK, Wang HS
Fertil. Steril., 2006-11-01;86(5):1392-401.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
Authors: Cortesio CL, Jiang W
Arch. Biochem. Biophys., 2006-03-03;449(1):164-70.
Species: Human
Sample Types: N/A
Applications: Enzyme Assay -
Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability.
Authors: Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A
Biochem. Biophys. Res. Commun., 2005-08-05;333(3):1011-6.
Species: Human
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, Enzyme Assay -
Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
J. Assist. Reprod. Genet., 2005-06-01;22(6):257-64.
Applications: ELISA (Standard)
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