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Recombinant Human IL-2 Heat Stable Agonist Protein, CF

Catalog #: BT-002HS Datasheet / COA / SDS

Engineered using artificial intelligence (AI), this protein has enhanced stability, enabling it to withstand high temperatures and extended culture durations. Learn more in this Application Note.

AI Modified Protein
Catalog # Availability Size / Price Qty
BT-002HS-010
BT-002HS-050
BT-002HS-100
BT-002HS-500
BT-002HS-01M
Proliferation bioassay in NK92 cells after 10 min at 95 °C
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Product Details
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-2 Heat Stable Agonist Protein, CF Summary

  • Simple to Use- Optimized for T Cells & Tumor Infiltrating Lymphocytes-IL-2 Heat Stable Agonist was modeled to engage only the IL-2 dimeric ßγ receptor complex
  • Improved Stability- Enhanced stability facilitated by a synthetic core engineered to strengthen the protein's structure, enabling it to withstand high temperatures and extended culture durations.
  • Superior Capabilities- Higher bioactivity at 37 oC compared to standard IL-2.

Product Specifications

Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NK-92 human natural killer lymphoma cells. The ED50 for this effect is 0.0150-0.150 ng/mL.
Source
E. coli-derived human IL-2 protein
Proprietary
Accession #
Engineered based on P60568.1
SDS-PAGE
7 kDa, under reducing conditions.

Product Datasheets

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BT-002HS

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-002HS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

After 10 min at 95°C (Control IL-2 C145S was kept at 4°C), only the heat stable IL-2 (Cat # BT-002HS) maintained bioactivity similar to the control IL-2, whereas the IL-2 Aldesleukin protein lost >14-fold activity.

Bioactivity View Larger

After 10 days at 10 ng/mL in the cell culture medium at 37°C (Control IL-2 C145S was kept at 4°C), only the heat stable IL-2 (Cat # BT-002HS) maintained bioactivity similar to the control IL-2, whereas the IL-2 Aldesleukin protein lost >3-fold activity.

Bioactivity View Larger

Recombinant Human IL 2 Heat Stable Agonist (Catalog # BT-002HS) stimulates proliferation of NK-92 human natural killer lymphoma cells. The ED50 for this effect is 0.0150‑0.150 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human IL‑2 Heat Stable Agonist Protein (Catalog # BT-002HS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 7 kDa.

Cell Culture View Larger

Heat Stable IL-2 (BT-002HS) Provides consistent CD4+/CD8+ T Cell Proliferation in Side-by-Side Testing with the Standard IL-2 Protein. CD4+ and CD8+ T cells were activated with anti-CD3-, anti-CD28- conjugated beads, and grown for 14 days in media containing 10 ng/mL Animal-free Recombinant Human IL-2 (R&D Systems, Catalog # BT-002-AFL) as the standard IL-2 protein or the AI modified Heat Stable IL-2. IL-2 conditions were refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days and showed that the Heat Stable IL-2 protein performs equally compared to the standard IL-2 protein.

Cell Culture View Larger

CD4+ and CD8+ T cells were activated with anti-CD3-, anti-CD28- conjugated beads, and grown for 14 days in media containing 10 ng/mL Animal-free Recombinant Human IL-2 (R&D Systems, Catalog # BT-002-AFL) as the standard IL-2 protein or Heat Stable IL-2 (Cat # BT-002HS). IL-2 conditions were refreshed every 2-3 days. After 21 days, the phenotypes of the cells were compared by flow cytometry using a PE-Cy7-conjugated mouse anti-human CD45RA monoclonal antibody and a Brilliant Violet 711 conjugated mouse anti-human CCR7 monoclonal antibody to determine the number of CD4+ and CD8+ naive (CD45RA+ CCR7+), central memory (CD45RA- CCR7+), terminal effector memory (CD45RA+ CCR7- ), and effector memory (CD45RA- CCR7- ) T cells. The percentages of each of these cell populations were found to be similar whether the cells were expanded with the standard or Heat Stable IL-2 protein.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2

Predictive analytics are increasingly effective in solving challenges associated with developing protein reagents for cell culture applications, including thermal and pH instability. We have improved the stability of IL-2 by generating a synthetic structural core while maintaining efficiency in engagement and activation of IL-2 R beta / gamma c receptors. The enhanced IL-2 displays improved heat stability enabling it to retain bioactivity while withstanding elevated temperatures and extended culture durations. Recombinant IL-2 is a bioactive protein for use in cell culture applications, often used for the expansion of T cells. IL-2 is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils (1-3).  rhIL-2 is also used for the expansion of NK cells, early differentiated T cells and effector memory Treg cells for adoptive cell transfer cancer immunotherapy (4,5).  rhIL-2 Heat Stable agonist is verified for activity using an NK92 proliferation assay as well as T Cell culture applications comparing to the commonly used mutant aldesleukin IL-2 version.

References
  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Koehl, U. et al. (2015) Oncoimmunology. 5:e1115178.
  5. Chamucero-Millares, J.A. et al. (2021) Cellular Immunol. 360:104257.
Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

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