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Recombinant Human IL-5 R alpha/CD125 Fc Avi-tag Protein, CF

Catalog #: AVI11073 Datasheet / COA / SDS
Biotinylated Fc Chimera
Catalog # Availability Size / Price Qty
AVI11073-025
Biotinylated Recombinant Human IL‑5 R alpha /CD125 Fc Chimera Avi-tag Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-5 R alpha/CD125 Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-5 (Catalog # 205-IL/CF) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-5 R alpha /CD125 Fc Chimera Avi-tag (Catalog # AVI11073) binds with an ED50 of 6.00-90.0 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human IL-5 R alpha/CD125 protein
Human IL-5RA
(Asp21-Glu335)
Accession # Q01344.2		IEGRMDHuman IgG1 Fc
(Pro100-Lys330)		Avi-tag
N-terminusC-terminus
Accession #
Q01344.2
N-terminal Sequence
Analysis
Asp21
Structure / Form
Disulfide-linked homodimer
Biotinylated via Avi-tag
Predicted Molecular Mass
64 kDa
SDS-PAGE
78-87 kDa, under reducing conditions.

Product Datasheets

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AVI11073

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11073

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human IL-5 (205-IL/CF) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-5 R alpha /CD125 Fc Chimera Avi-tag Protein (Catalog # AVI11073) binds with an ED50 of 6.00-90.0 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human IL‑5 R alpha /CD125 Fc Chimera Avi-tag Protein (Catalog # AVI11073) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-87 kDa and 160-170 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-5 R alpha/CD125

Interleukin‑5 Receptor alpha (IL‑5 R alpha ), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1‑3). Mature human IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 20 aa transmembrane segment, and a 58 aa cytoplasmic domain (4, 5). Within the ECD, human IL-5 R alpha shares 71% aa sequence identity with mouse and rat IL‑5 R alpha. Alternate splicing of human IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists (5‑7). The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common beta chain ( beta c/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF (4). IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed beta c oligomers to form the signaling‑competent receptor complex (8). IL‑5 stimulation of CD34+ hematopoietic progenitor cells induces the up‑regulation of transmembrane IL‑5 R alpha followed by eosinophilic differentiation and activation (9 ‑ 11). IL‑5 R alpha also promotes the differentiation of basophils and B cells (12, 13). Exposure of mature eosinophils to IL‑5 attenuates their IL‑5 responsiveness by inducing the down‑regulation of surface IL‑5 R alpha and increased production of soluble IL‑5 R alpha (14, 15). Elevated production of IL‑5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3). Our Avi-tag Biotinylated human IL-5 R alpha Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Rothenberg, M.E. and S.P. Hogan (2005) Annu. Rev. Immunol. 24:147.
  3. Elsas, X.P. and M.I.G. Elsas (2007) Curr. Med. Chem. 14:1925.
  4. Tavernier, J. et al. (1991) Cell 66:1175.
  5. Murata, Y. et al. (1992) J. Exp. Med. 175:341.
  6. Tavernier, J. et al. (1992) Proc. Natl. Acad. Sci. 89:7041.
  7. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  8. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  9. Tavernier, J. et al. (2000) Blood 95:1600.
  10. Clutterbuck, E.J. et al. (1989) Blood 73:1504.
  11. Lopez, A.F. et al. (1988) J. Exp. Med. 167:219.
  12. Denburg, J.A. et al. (1991) Blood 77:1462.
  13. Hasbold, J. et al. (2004) Nat. Immunol. 5:55.
  14. Gregory, B. et al. (2003) J. Immunol. 170:5359.
  15. Liu, L.Y. et al. (2002) J. Immunol. 169:6459.
Long Name
Interleukin 5 Receptor alpha
Entrez Gene IDs
3568 (Human); 16192 (Mouse)
Alternate Names
CD125 antigen; CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5 receptor subunit alpha; IL5R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; IL-5R-alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor alpha subunit; interleukin-5 receptor subunit alpha; MGC26560

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