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Recombinant Human IL-5 R alpha Protein

Catalog #: 253-5R Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
253-5R-025/CF

With Carrier

Catalog # Availability Size / Price Qty
253-5R-025
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Recombinant Human IL-5 R alpha Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-5-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.5-2 µg/mL in the presence of 0.5 ng/mL of rhIL-5.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-5 R alpha/CD125 protein
Asp21-Glu335
Accession #
Q01344
N-terminal Sequence
Analysis
Asp21
Predicted Molecular Mass
38 kDa
SDS-PAGE
43 kDa, reducing conditions

Product Datasheets

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253-5R (with carrier)

Product Datasheet
COA
SDS

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253-5R/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

253-5R

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

253-5R/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-5 R alpha/CD125

Interleukin-5 Receptor alpha (IL-5 R alpha ), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1 - 3). Mature human IL-5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 20 aa transmembrane segment, and a 58 aa cytoplasmic domain (4, 5). Within the ECD, human IL-5 R alpha shares 71% aa sequence identity with mouse and rat IL-5 R alpha. Alternate splicing of human IL-5 R alpha generates soluble secreted forms which function as IL-5 antagonists (5 - 7). The high affinity receptor for IL-5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common beta chain ( beta c/CD131) which is shared with the receptor complexes for IL-3 and GM-CSF (4). IL-5 R alpha binds IL-5 at low affinity and then associates with preformed beta c oligomers to form the signaling-competent receptor complex (8). IL-5 stimulation of CD34+ hematopoietic progenitor cells induces the upregulation of transmembrane IL-5 R alpha followed by eosinophilic differentiation and activation (9 - 11). IL‑5 R alpha also promotes the differentiation of basophils and B cells (12, 13). Exposure of mature eosinophils to IL-5 attenuates their IL-5 responsiveness by inducing the downregulation of surface IL-5 R alpha and increased production of soluble IL-5 R alpha (14, 15). Elevated production of IL-5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3).

References
  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Rothenberg, M.E. and S.P. Hogan (2005) Annu. Rev. Immunol. 24:147.
  3. Elsas, X.P. and M.I.G. Elsas (2007) Curr. Med. Chem. 14:1925.
  4. Tavernier, J. et al. (1991) Cell 66:1175.
  5. Murata, Y. et al. (1992) J. Exp. Med. 175:341.
  6. Tavernier, J. et al. (1992) Proc. Natl. Acad. Sci. 89:7041.
  7. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  8. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  9. Tavernier, J. et al. (2000) Blood 95:1600.
  10. Clutterbuck, E.J. et al. (1989) Blood 73:1504.
  11. Lopez, A.F. et al. (1988) J. Exp. Med. 167:219.
  12. Denburg, J.A. et al. (1991) Blood 77:1462.
  13. Hasbold, J. et al. (2004) Nat. Immunol. 5:55.
  14. Gregory, B. et al. (2003) J. Immunol. 170:5359.
  15. Liu, L.Y. et al. (2002) J. Immunol. 169:6459.
Long Name
Interleukin 5 Receptor alpha
Entrez Gene IDs
3568 (Human); 16192 (Mouse)
Alternate Names
CD125 antigen; CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5 receptor subunit alpha; IL5R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; IL-5R-alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor alpha subunit; interleukin-5 receptor subunit alpha; MGC26560

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