Recombinant Human MCAM/CD146 Fc Chimera Protein, CF Summary
Product Specifications
| Human MCAM/CD146 (Val24-Gly559) Accession # AAA20922 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9709-MA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human MCAM/CD146 Fc Chimera is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Galectin-3 (Catalog # 8259-GA) binds with an ED50 of 0.25‑1.25 ug/mL.
Reconstitution Calculator
Background: MCAM/CD146
Melanoma cell adhesion molecule (MCAM), also known as MUC18 or CD146, is a putative adhesion molecule that belongs to the immunoglobulin superfamily (IgSF) (1). MCAM is an approximately 113 kDa type I transmembrane glycoprotein that contains a 536 amino acid (aa) extracellular domain (ECD), a 24 aa transmembrane domain, and a 63 aa cytoplasmic domain. Two MCAM splice variants have been observed, which vary in the length of their cytoplasmic tail (2). The ECD of human MCAM contains 2 IgV and 3 IgC2 domains and shares 74% and 73% identity with mouse and rat, respectively. MCAM was originally described as a marker of malignant potential in melanoma and was reported to promote both invasion and metastasis (3). Since then, expression has been detected in endothelial cells throughout the body and MCAM has been shown to be involved multiple cellular events including adhesion, migration, proliferation and differentiation (4, 5). Additionally, MCAM has been implicated in recruitment of activated T cells to inflammatory sites and is up-regulated in various inflammatory diseases (5, 6). Inhibiting MCAM signaling has been suggested as a potential therapy for diverse diseases including inflammatory bowel disease and ovarian cancer (7, 8). As a cellular adhesion molecule (CAM), MCAM functions as a molecular mediator to facilitate inter-cellular interactions of homotypic or heterotypic cells, or to intervene in interactions of cell-to-extracellular matrix for responding to physiological signal (9). MCAM has also been shown to be the functional ligand for Galectin-3 on endothelial cell surfaces (9).
- Lehmann, J.M. et al. (1989) Proc Natl Acad Sci U.S.A. 86:9891.
- Alais, S. et al. (2001) J. Cell Sci. 114:1847.
- Shih, I.M. et al. (1994) Am J Pathol. 145:837.
- Schrage, A. et al. (2008) Histochem Cell Biol. 129:441.
- Wang Z and Yan X. (2013) Cancer Lett. 330:150.
- Bardin, N. et al. (2006) Inflamm Bowel Dis. 12:16.
- Xing, S. et al. (2014) Am J Pathol. 184:1604.
- Ma, X. et al. (2017) Oncol Lett. 13:1681.
- Colomb F, et al. (2017) J Biol Chem. 292:8381.
Citations for Recombinant Human MCAM/CD146 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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CD146/Soluble CD146 Pathway Is a Novel Biomarker of Angiogenesis and Inflammation in Proliferative Diabetic Retinopathy
Authors: AM Abu El-Asr, MI Nawaz, A Ahmad, MM Siddiquei, E Allegaert, PW Gikandi, G De Hertogh, G Opdenakker
Investigative Ophthalmology & Visual Science, 2021-07-01;62(9):32.
Species: Human, Rat
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application.
Authors: Malara N, Trunzo V, Musolino G, Aprigliano S, Rotta G, Macrina L, Limongi T, Gratteri S, Di Fabrizio E, Renzulli A, Fini M, Mollace V
Int J Cardiol Heart Vasc, 2015-01-09;6(0):48-53.
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