Recombinant Human Melanotransferrin/CD228 His Protein, CF

Recombinant Human Melanotransferrin/CD228 His Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Melanotransferrin/CD228

Melanotransferrin (MTF), also known as CD228, P97, MELTF, or MFI2, is a member of the transferrin superfamily that binds to a single ferric iron with high affinity (1). It was originally identified as a membrane-bound protein with a coding region of 2217 bases encoding 738 amino acids (aa) including five Fe-binding residues, the consensus thermolysin metalloprotease sites, and a glycosyl-phosphatidylinositol (GPI) anchor (2). A secreted form of MTF (sMTF) has also been reported (2, 3). Human MTF shares 86% aa sequence identity with mouse and rat MTF. MTF expression is low in normal tissues but high in tumor and embryonic tissues. In melanoma MTF is associated with tumor metastasis and angiogenesis and upregulated in lung cancer tissue and cell lines (3). In the brain MTF is expressed in capillary endothelium but also in the reactive microglia associated with senile plaques in Alzheimer's disease (AD) (4). sMTF has been reported to be increased in patients with AD or arthritis and may modulate angiogenesis, cell migration, and plasminogen activation (5).

Related Research Areas

• Cancer Biomarkers