Home / Methionine Aminopeptidase 2/METAP2 / Recombinant Human Methionine Aminopeptidase 2 Protein, CF

Recombinant Human Methionine Aminopeptidase 2 Protein, CF

Catalog #: 3795-ZN
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
3795-ZN-020
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Methionine Aminopeptidase 2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 9168-SE). The specific activity is >15 pmol/min/µg, as measured using the described conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Methionine Aminopeptidase 2/METAP2 protein
Ala2-Tyr478, with a C-terminal 10-His tag
Accession #
P50579
N-terminal Sequence
Analysis
Ala2
Predicted Molecular Mass
54 kDa
SDS-PAGE
66 kDa, reducing conditions

Product Datasheets

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3795-ZN

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3795-ZN

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Assay Procedure

Materials
  • Assay Buffer: 50 mM HEPES, 0.1 mM CoCl2, 100 mM NaCl, pH 7.5
  • Recombinant Human Methionine Aminopeptidase 2/METAP2 (rhMETAP2) (rhMETAP2) (Catalog # 3795-ZN
  • Recombinant Human DPPIV/CD26 (rhCD26) (Catalog # 9168-SE)
  • Substrate: Met-Gly-Pro-AMC (Catalog # ES017)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhMETAP2 to 10 µg/mL in Assay Buffer.
  2. Dilute Substrate to 500 µM with 2 µg/mL rhCD26 in Assay Buffer.
  3. Load 50 µL of 10 µg/mL rhMETAP2 into a plate, and start the reaction by adding 50 µL of Substrate/rhCD26 mixture.
  4. Incubate at room temperature for 10 minutes.
  5. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes (effectively reading timepoints between 10 and 15 minutes).
  6. Calculate specific activity using the following formula:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-Amino-4-Methyl-Courmarin (AMC) (Sigma, Catalog#: A-9891).

Per Well:
  • rhMETAP2: 0.500 µg
  • rhCD26: 0.100 µg
  • Substrate: 250 µM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Methionine Aminopeptidase 2/METAP2

The human METAP2 gene encodes Methionine Aminopeptidase 2, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). METAP2 plays an important role in the development of different types of cancer (3) and has been a novel target for developing anti-cancer drugs.

References
  1. Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta 1477:157.
  2. Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
  3. Selvakumar, P. et al. (2006) Biochim. Biophys. Acta 1765:148.
Entrez Gene IDs
10988 (Human); 56307 (Mouse); 64370 (Rat)
Alternate Names
Amp2; eIF-2-associated p67 homolog; Initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MetAP 2; METAP2; Methionine Aminopeptidase 2; methionyl aminopeptidase 2; MNPEP; p67; p67EC 3.4.11.18; p67eIF2; Peptidase M 2

Product Specific Notices

This product is covered by US Patent Nos. 5,885,820 and 5,888,796 licensed from Mediomics, LLC.

Citations for Recombinant Human Methionine Aminopeptidase 2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Tunable, biodegradable grafting-from glycopolypeptide bottlebrush polymers
    Authors: ZS Clauss, CL Wardzala, AE Schlirf, NS Wright, SS Saini, B Onoa, C Bustamante, JR Kramer
    Nature Communications, 2021-11-09;12(1):6472.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Neutral metalloaminopeptidases APN and MetAP2 as newly discovered anticancer molecular targets of actinomycin D and its simple analogs
    Authors: E W?glarz-To, M Talma, M Giurg, HV Westerhoff, R Janowski, A Mucha
    Oncotarget, 2018-06-29;9(50):29365-29378.
    Species: Human
    Sample Types: Aminopeptidase
    Applications: Enzyme Assay
  3. Redox regulation of methionine aminopeptidase 2 activity.
    Authors: Chiu J, Wong J, Hogg P
    J Biol Chem, 2014-04-03;289(21):15035-43.
    Applications: Bioassay

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