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Recombinant Human NELL2 Protein, CF

Catalog #: 8946-NL
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8946-NL-050
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Recombinant Human NELL2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.3-1.8 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human NELL2 protein
Leu22-Leu816, with a C-terminal 6-His tag
Accession #
Q99435
N-terminal Sequence
Analysis
Leu22
Predicted Molecular Mass
90 kDa
SDS-PAGE
100-125 kDa, reducing conditions

Product Datasheets

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8946-NL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8946-NL

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Citrate, NaCl and EDTA.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NELL2

NELL2 is an approximately 140 kDa secreted glycoprotein that exhibits several actions during nervous system development. NELL2 associates into a homotrimer and contains one Laminin G domain, one vWF-C domain, and six EGF-like domains, followed by two more vWF-C domains (1). Mature human NELL2 shares 94% aa sequence identity with mouse and rat NELL2. Alternative splicing generates a cytosolic isoform that associates with PKC beta 1 and can inhibit PKC signaling (2). NELL2 is widely expressed in neurons of the central and peripheral nervous systems, particularly in the hippocampus, hypothalamus, cerebral cortex, and cerebellar cortex (3-5). It is selectively expressed in VGAT1+ or VGAT2+ glutamatergic neurons (4, 6). During development, NELL2 is expressed in select neural crest cells, retinal ganglion cells, and hypaxial muscle precursor cells of the dermomyotome (7). It promotes the differentiation and migration of both motor and sensory neurons (8), enhances neuronal survival (9), and promotes neuronal aggregation by inducing N-Cadherin up-regulation (10). NELL2 supports the release of GnRH from the hypothalamus which is important for the onset of female puberty (5, 6). NELL2 is additionally expressed in renal tubules but is down-regulated in renal cancer (11).

References
  1. Watanabe, T.K. et al. (1996) Genomics 38:273.
  2. Lee, D.Y. et al. (2014) Biochem. Biophys. Res. Commun. 454:459.
  3. Oyasu, M. et al. (2000) Brain Res. Mol. Brain Res. 76:151.
  4. Jeong, J.K. et al. (2008) Mol. Cells 26:186.
  5. Zhou, S.S. and P. Li (2014) Genet. Mol. Res. 13:6672.
  6. Ha, C.M. et al. (2008) Neuroendocrinology 88:199.
  7. Nelson, B.R. et al. (2002) Mech. Dev. 119S:S11.
  8. Nelson, B.R. et al. (2004) Dev. Biol. 270:322.
  9. Aihara, K. et al. (2003) Mol. Brain Res. 116:86.
  10. Kim, D.H. et al. (2014) PLoS One 9:e85898.
  11. Nakamura, R. et al. (2015) Cancer Sci. 106:656.
Long Name
NEL-like 2
Entrez Gene IDs
4753 (Human); 54003 (Mouse); 81734 (Rat)
Alternate Names
Mel91; nel (chicken)-like 2; NELL2; NEL-like 2 (chicken); NEL-like protein 2; Nel-related protein 2; NRP2; NRP2neural epidermal growth factor-like 2; protein kinase C-binding protein NELL2

Citation for Recombinant Human NELL2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growth
    Authors: P Jayabal, F Zhou, X Lei, X Ma, B Blackman, ST Weintraub, PJ Houghton, Y Shiio
    Cell Reports, 2021-07-06;36(1):109254.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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